Comprehensive proteome analyses of lysine acetylation in tea leaves by sensing nitrogen nutrition

Jiang, Jutang; Gai, Zhongshuai; Wang, Yu; Fan, Kai; Sun, Litao; Wang, Hui; Ding, Zhaotang

doi:10.1186/s12864-018-5250-4

Cited by 45 publications

(54 citation statements)

References 39 publications

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“…By GO functional analysis of the acetylated proteins, we found “protein binding” was one of the most abundant terms in the MF category in A. japonicus , which was consistent with the findings in bacteria [23], plants [25], and mammals [24]. The terms associated with “ribosome”, “nucleus”, “cytoplasm”, and “membrane” were enriched in the CC category, which suggested the wide distribution of lysine acetylated proteins [25]. The acetylated proteins we identified also represented a wide range of BP terms.…”

Section: Discussionsupporting

confidence: 87%

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Lysine Acetylation is an Important Post-Translational Modification that Modulates Heat Shock Response in the Sea Cucumber Apostichopus japonicus

Wang

2019

IJMS

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Heat stress (HS) is an important factor for the survival of the marine organism Apostichopus japonicus. Lysine acetylation is a pivotal post-translational modification that modulates diverse physiological processes including heat shock response (HSR). In this study, 4028 lysine acetylation sites in 1439 proteins were identified in A. japonicus by acetylproteome sequencing. A total of 13 motifs were characterized around the acetylated lysine sites. Gene Ontology analysis showed that major acetylated protein groups were involved in “oxidation–reduction process”, “ribosome”, and “protein binding” terms. Compared to the control group, the acetylation quantitation of 25 and 41 lysine sites changed after 6 and 48 h HS. Notably, lysine acetyltransferase CREB-binding protein (CBP) was identified to have differential acetylation quantitation at multiple lysine sites under HS. Various chaperones, such as caseinolytic peptidase B protein homolog (CLBP), T-complex protein 1 (TCP1), and cyclophilin A (CYP1), showed differential acetylation quantitation after 48 h HS. Additionally, many translation-associated proteins, such as ribosomal proteins, translation initiation factor (IF), and elongation factors (EFs), had differential acetylation quantitation under HS. These proteins represented specific interaction networks. Collectively, our results offer novel insight into the complex HSR in A. japonicus and provide a resource for further mechanistic studies examining the regulation of protein function by lysine acetylation.

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Section: Discussionsupporting

confidence: 87%

“…Sequence logos showed that lysine was over-represented immediately around acetylated lysine in A. japonicus . The preference was also observed in many other species [25,26]. These motifs may be specially recognized by diverse lysine acetyltransferases (KATs).…”

Section: Discussionmentioning

confidence: 76%

Lysine Acetylation is an Important Post-Translational Modification that Modulates Heat Shock Response in the Sea Cucumber Apostichopus japonicus

Wang

2019

IJMS

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“…Moreover, previous study in our laboratory showed that the levels of lysine acetylation in tea leaves changed dynamically under NH 4 + resupply. And lysine acetylated proteins might regulate photosynthesis, glycolysis and secondary metabolism in tea leaves after NH 4 + resupply [5]. However, the global profiling of lysine crotonylated proteins in tea plants in response to NH 4 + resupply remains largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Ammonium triggered the response mechanism of lysine crotonylome in tea plants

et al. 2019

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Background Lysine crotonylation, as a novel evolutionarily conserved type of post-translational modifications, is ubiquitous and essential in cell biology. However, its functions in tea plants are largely unknown, and the full functions of lysine crotonylated proteins of tea plants in nitrogen absorption and assimilation remains unclear. Our study attempts to describe the global profiling of nonhistone lysine crotonylation in tea leaves and to explore how ammonium (NH 4 + ) triggers the response mechanism of lysine crotonylome in tea plants. Results Here, we performed the global analysis of crotonylome in tea leaves under NH 4 + deficiency/resupply using high-resolution LC-MS/MS coupled with highly sensitive immune-antibody. A total of 2288 lysine crotonylation sites on 971 proteins were identified, of which contained in 15 types of crotonylated motifs. Most of crotonylated proteins were located in chloroplast (37%) and cytoplasm (33%). Compared with NH 4 + deficiency, 120 and 151 crotonylated proteins were significantly changed at 3 h and 3 days of NH 4 + resupply, respectively. Bioinformatics analysis showed that differentially expressed crotonylated proteins participated in diverse biological processes such as photosynthesis (PsbO, PsbP, PsbQ, Pbs27, PsaN, PsaF, FNR and ATPase), carbon fixation (rbcs, rbcl, TK, ALDO, PGK and PRK) and amino acid metabolism (SGAT, GGAT2, SHMT4 and GDC), suggesting that lysine crotonylation played important roles in these processes. Moreover, the protein-protein interaction analysis revealed that the interactions of identified crotonylated proteins diversely involved in photosynthesis, carbon fixation and amino acid metabolism. Interestingly, a large number of enzymes were crotonylated, such as Rubisco, TK, SGAT and GGAT, and their activities and crotonylation levels changed significantly by sensing ammonium, indicating a potential function of crotonylation in the regulation of enzyme activities. Conclusions The results indicated that the crotonylated proteins had a profound influence on metabolic process of tea leaves in response to NH 4 + deficiency/resupply, which mainly involved in diverse aspects of primary metabolic processes by sensing NH 4 + , especially in photosynthesis, carbon fixation and amino acid metabolism. The data might serve as important resources for exploring the roles of lysine crotonylation in N metabolism of tea plants. Data were available via ProteomeXchange with identifier PXD011610. Electronic supplementary material The online version of this article (10.1186/s12864-019-5716-z) contains supplementary material, which is available to authorized users.

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“…Tea plant cultivar, ‘QN3’ ( Camellia sinensis cv. QN3) is an improved cultivar bred by the Tea Research Institute, Qingdao Agricultural University 43 – 45 . The 2-year-old tea seedling were cultured in growth chamber for 2 weeks with photoperiod (12 h light at 25°C and 12 h dark at 20°C, 75% of humidity) and a light intensity of 18, 000 lx.…”

Section: Methodsmentioning

confidence: 99%

Exogenous abscisic acid induces the lipid and flavonoid metabolism of tea plants under drought stress

Gai

Wang

Ding

et al. 2020

Sci Rep

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110

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Abscisic acid (ABA) is an important phytohormone responsible for activating drought resistance, but the regulation mechanism of exogenous ABA on tea plants under drought stress was rarely reported. Here, we analyzed the effects of exogenous ABA on genes and metabolites of tea leaves under drought stress using transcriptomic and metabolomic analysis. The results showed that the exogenous ABA significantly induced the metabolic pathways of tea leaves under drought stress, including energy metabolism, amino acid metabolism, lipid metabolism and flavonoids biosynthesis. In which, the exogenous ABA could clearly affect the expression of genes involved in lipid metabolism and flavonoid biosynthesis. Meanwhile, it also increased the contents of flavone, anthocyanins, flavonol, isoflavone of tea leaves under drought stress, including, kaempferitrin, sakuranetin, kaempferol, and decreased the contents of glycerophospholipids, glycerolipids and fatty acids of tea leaves under drought stress. The results suggested that the exogenous ABA could alleviate the damages of tea leaves under drought stress through inducing the expression of the genes and altering the contents of metabolites in response to drought stress. This study will be helpful to understand the mechanism of resilience to abiotic stress in tea plant and provide novel insights into enhancing drought tolerance in the future.

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Comprehensive proteome analyses of lysine acetylation in tea leaves by sensing nitrogen nutrition

Cited by 45 publications

References 39 publications

Lysine Acetylation is an Important Post-Translational Modification that Modulates Heat Shock Response in the Sea Cucumber Apostichopus japonicus

Lysine Acetylation is an Important Post-Translational Modification that Modulates Heat Shock Response in the Sea Cucumber Apostichopus japonicus

Ammonium triggered the response mechanism of lysine crotonylome in tea plants

Exogenous abscisic acid induces the lipid and flavonoid metabolism of tea plants under drought stress

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