Compressive properties of cartilage-like tissues repaired in vivo with scaffold-free, tissue engineered constructs

Katakai, Daisuke; Imura, Machiko; Ando, Wataru; Tateishi, Kosuke; Yoshikawa, Hideki; Nakamura, Norimasa; Fujie, Hiromichi

doi:10.1016/j.clinbiomech.2008.07.003

Cited by 22 publications

(20 citation statements)

References 24 publications

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“…Generally, tissue engineering has involved the incorporation of cells into a scaffold; however, there has been a recent move toward a scaffold-free approach in order to avoid issues of biocompatibility, cell attachment, porosity, rate of bioresorption, cytotoxicity of degradation products, and stress-shielding (Hu and Athanasiou, 2006b). Numerous approaches to forming scaffold-free constructs have been described: pellet culture (Jin et al, 2009), active tissue contraction (Ando et al, 2007;Katakai et al, 2009), transwells (Elder et al, 2009;Hayes et al, 2007;Murdoch et al, 2007;Naumann et al, 2004), custom molds (Han et al, 2008;Jubel et al, 2008;Nagai et al, 2008;Stoddart et al, 2006a), and agarose wells (Elder and Kyriacos, 2008;Hu and Athanasiou, 2006a). The size of these scaffold-free constructs range from 0.071 to 0.785 cm 2 with an average size of 0.365 cm 2 .…”

Section: Introductionmentioning

confidence: 99%

Effect of a mechanical stimulation bioreactor on tissue engineered, scaffold‐free cartilage

Tran

Cooley

Elder

2011

Biotech & Bioengineering

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Achieving sufficient functional properties prior to implantation remains a significant challenge for the development of tissue engineered cartilage. Many studies have shown chondrocytes respond well to various mechanical stimuli, resulting in the development of bioreactors capable of transmitting forces to articular cartilage in vitro. In this study, we describe the production of sizeable, tissue engineered cartilage using a novel scaffold-free approach, and determine the effect of perfusion and mechanical stimulation from a C9-x Cartigen bioreactor on the properties of the tissue engineered cartilage. We created sizable tissue engineered cartilage from porcine chondrocytes using a scaffold-free approach by centrifuging a high-density chondrocyte cell-suspension onto an agarose layer in a 50 mL tube. The gross and histological appearances, biochemical content, and mechanical properties of constructs cultured in the bioreactor for 4 weeks were compared to constructs cultured statically. Mechanical properties were determined from unconfined uniaxial compression tests. Constructs cultured in the bioreactor exhibited an increase in total GAG content, equilibrium compressive modulus, and dynamic modulus versus static constructs. Our study demonstrates the C9-x CartiGen bioreactor is able to enhance the biomechanical and biochemical properties of scaffold-free tissue engineered cartilage; however, no additional enhancement was seen between loaded and perfused groups.

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Section: Introductionmentioning

confidence: 99%

Effect of a mechanical stimulation bioreactor on tissue engineered, scaffold‐free cartilage

Tran

Cooley

Elder

2011

Biotech & Bioengineering

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“…However, the value was lower than compressive modulus of human and animal cartilage specimens tested, which was varying between 200 and 1,200 kPa (Athanasiou et al 1991;Eisenberg and Grodzinsky 1985;Jurvelin et al 2003;Katakai et al 2009;Mow et al 1980). Since the compressive modulus is a material property that is strongly dependent on solid content, it is possible to improve solid content by optimizing the fabrication process and thus the compressive modulus applicable for tissue engineering of cartilage.…”

Section: Mechanical Propertiesmentioning

confidence: 80%

Microstructure and mechanical properties of bacterial cellulose/chitosan porous scaffold

et al. 2010

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A family of polysaccharide based scaffold materials, bacterial cellulose/chitosan (BC/CTS) porous scaffolds with various weight ratios (from 20/80 to 60/40 w/w%) were prepared by freezing (-30 and -80°C) and lyophilization of a mixture of microfibrillated BC suspension and chitosan solution. The microfibrillated BC (MFC) was subjected to 2,2,6,6-tetramethylpyperidine-1-oxyl radical (TEMPO)-mediated oxidation to introduce surface carboxyl groups before mixing. The integration of MFC within chitosan matrix was performed by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC)-mediated cross-linking. The covalent amide bond formation was determined by ATR-FTIR. Because of this covalent coupling, the scaffolds retain their original shapes during autoclave sterilization. The composite scaffolds are three-dimensional open pore microstructure with pore size ranging from 120 to 280 lm. The freezing temperature and mean pore size take less effect on scaffold mechanical properties. The compressive modulus and strength increased with increase in MFC content. The results show that the scaffolds of higher MFC content contribute to overall better mechanical properties.

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“…Partial to robust healing of the OCD takes place over weeks to months [ 110 ] . The cartilage produced by these cells was very much like native hyaline cartilage, but subtle differences have been observed [ 138 ] .…”

Section: Use Of Msc In Musculoskeltal Diathesismentioning

confidence: 97%

Treatment of Chronic Painful Musculoskeletal Injuries and Diseases with Regenerative Injection Therapy (RIT): Regenerative Injection Therapy Principles and Practice

Linetsky¹,

Alfredson²,

Crane³

et al. 2012

Comprehensive Treatment of Chronic Pain by Medical, Interventional, and Integrative Approaches

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Compressive properties of cartilage-like tissues repaired in vivo with scaffold-free, tissue engineered constructs

Cited by 22 publications

References 24 publications

Effect of a mechanical stimulation bioreactor on tissue engineered, scaffold‐free cartilage

Effect of a mechanical stimulation bioreactor on tissue engineered, scaffold‐free cartilage

Microstructure and mechanical properties of bacterial cellulose/chitosan porous scaffold

Treatment of Chronic Painful Musculoskeletal Injuries and Diseases with Regenerative Injection Therapy (RIT): Regenerative Injection Therapy Principles and Practice

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