Computation of response factors for quantitative analysis of monoterpenes by gas-liquid chromatography

Raffa, Kenneth F.; Steffeck, Robert J.

doi:10.1007/bf01020142

Cited by 35 publications

(20 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Raffa and Steffeck (1988) had similar difficulties in their study. The molecular structure of myrcene is similar to that of the ocimenes (they are both acyclic monoterpenes), which Komenda et al (2001) also had difficulty quantifying.…”

Section: Resultsmentioning

confidence: 92%

“…Additionally. Raffa and Steffeck (1988) had difficulty quantifying myrcene and they neglected to run any terpenoid derivatives that are also likely to be present in any environmental samples. Another relevant study was published by Komenda et al (2001).…”

Section: L Faiola Et Al: Quantification Of Biogenic Volatile Orgmentioning

confidence: 99%

“…However, there has been some previous work related to the topic. In one, Raffa and Steffeck (1988) ran ten liquid monoterpene standards and calculated FID response factors for each terpene relative to n-pentane. They found that each monoterpene had a response factor close to one, i.e.…”

Section: L Faiola Et Al: Quantification Of Biogenic Volatile Orgmentioning

confidence: 99%

See 2 more Smart Citations

Quantification of biogenic volatile organic compounds with a flame ionization detector using the effective carbon number concept

et al. 2012

View full text Add to dashboard Cite

Abstract. Biogenic volatile organic compounds (BVOCs) are emitted into the atmosphere by plants and include isoprene, monoterpenes, sesquiterpenes, and their oxygenated derivatives. These BVOCs are among the principal factors influencing the oxidative capacity of the atmosphere in forested regions. BVOC emission rates are often measured by collecting samples onto adsorptive cartridges in the field and then transporting these samples to the laboratory for chromatographic analysis. One of the most commonly used detectors in chromatographic analysis is the flame ionization detector (FID). For quantitative analysis with an FID, relative response factors may be estimated using the effective carbon number (ECN) concept. The purpose of this study was to determine the ECN for a variety of terpenoid compounds to enable improved quantification of BVOC measurements. A dynamic dilution system was developed to make quantitative gas standards of VOCs with mixing ratios from 20-55 ppb. For each experiment using this system, one terpene standard was co-injected with an internal reference, noctane, and analyzed via an automated cryofocusing system interfaced to a gas chromatograph flame ionization detector and mass spectrometer (GC/MS/FID). The ECNs of 16 compounds (14 BVOCs) were evaluated with this approach, with each test compound analyzed at least three times. The difference between the actual carbon number and measured ECN ranged from −24 % to −2 %. The difference between theoretical ECN and measured ECN ranged from −22 % to 9 %. Measured ECN values were within 10 % of theoretical ECN values for most terpenoid compounds.

show abstract

Section: Resultsmentioning

confidence: 92%

Section: L Faiola Et Al: Quantification Of Biogenic Volatile Orgmentioning

confidence: 99%

See 1 more Smart Citation

Quantification of biogenic volatile organic compounds with a flame ionization detector using the effective carbon number concept

et al. 2012

View full text Add to dashboard Cite

show abstract

“…This internal standard has been commonly used in previous, including recent, analyses of these compounds (Robert et al, 2010;Boone et al, 2011), thereby facilitating between-study comparisons. However, some variation could arise from different response factors to any common internal standard (Raffa and Steffeck, 1988), and likely increases when non-monoterpene hydrocarbons are included. The remaining phloem was dried at 25°C for 1 wk and weighed.…”

Section: Effects Of Fire Injury On Constitutive and Induced Volatilementioning

confidence: 98%

Fire Injury Reduces Inducible Defenses of Lodgepole Pine against Mountain Pine Beetle

Powell

Raffa

2011

J Chem Ecol

Self Cite

View full text Add to dashboard Cite

We examined the effect of wildfire injury on lodgepole pine chemical defenses against mountain pine beetle. We compared the constitutive phloem chemistry among uninjured, lightly-, moderately-, and severely-injured trees, and the induced chemistry elicited by simulated beetle attack, among these same categories. We also compared the entry rates of caged female beetles into trees of these categories. The volatiles we studied included thirteen monoterpene hydrocarbons, four allylic monoterpene alcohols, one ester, and one phenyl propanoid, of which the monoterpene hydrocarbons always comprised 96% or more of the total. Fire injury reduced the total concentration of these compounds in the induced but not constitutive phloem tissue of lodgepole pines. Fire injury also affected the relative composition of some volatiles in both induced and constitutive phloem. For example, increased fire injury reduced 4-allylanisole, a host compound that inhibits mountain pine beetle aggregation. Increased fire injury also increased (-) α-pinene, which can serve as precursor of pheromone communication. However, it also reduced myrcene and terpinolene, which can serve as stimulants and synergists of pheromone communication. Beetle entry did not show statistical differences among fire injury categories, although there was a trend to increased entry with fire injury. These results suggest that the reduced ability of trees to mobilize induced chemical defenses is an important mechanism behind the higher incidence of attack on fire-injured trees in the field. Future studies should concentrate on whether beetles that enter fire-injured trees are more likely to elicit aggregation, based on the differences we observed in volatile composition.

show abstract

“…The carrier gas, helium, was maintained at 30 cm/s. Compounds were identiÞed based on retention times from known samples (Raffa and Steffeck 1988).…”

Section: Methodsmentioning

confidence: 99%

Variation in Complex Semiochemical Signals Arising From Insects and Host Plants

et al. 2010

Self Cite

View full text Add to dashboard Cite

Chemical communication by many insect species involves complex signals of both insect and plant origin. Much attention has been focused on the behavioral activities of these components but less on their sources of variation, despite implications for evolutionary theory and pest management. We studied variation in chemical signaling at host, tree-within-host, and beetle-on-tree scales using tunneling male pine engravers [Ips pini (Say)] on jack, Pinus banksiana Lamb, red, P. resinosa Aiton, and white, P. strobus L. pines. Pine engravers are distributed transcontinentally, and stereoisomeric ratios of their principal pheromone component ipsdienol varies regionally. Linear mixed-effects models were used to examine variation in monoterpene and pheromone volatile profiles, determined by gas chromatography. Phloem from white pine had the greatest concentration of monoterpenes, although insects tunneling in white pine produced the smallest ratios of monoterpenes to pheromones (1:2) in their volatile plumes relative to jack and red pine (1:1). Beetle-to-beetle variation in plume composition was approximately 2-9 times greater than the inter-tree variation within a tree species. The stereoisomeric ratio of ipsdienol was highly consistent within the pheromone component of the plume. The little variation present existed almost entirely at the level of the insects. Within the pheromone component of the plume in a given host species, there was up to 13 times more beetle-to-beetle than tree-to-tree variation. This magnitude was almost double the magnitudes of the ratios among components within the entire plumes. Implications to the behavioral ecology of bark beetle communication, such as potential strategies of cheating and predator avoidance, are discussed.

show abstract

Computation of response factors for quantitative analysis of monoterpenes by gas-liquid chromatography

Cited by 35 publications

References 9 publications

Quantification of biogenic volatile organic compounds with a flame ionization detector using the effective carbon number concept

Quantification of biogenic volatile organic compounds with a flame ionization detector using the effective carbon number concept

Fire Injury Reduces Inducible Defenses of Lodgepole Pine against Mountain Pine Beetle

Variation in Complex Semiochemical Signals Arising From Insects and Host Plants

Contact Info

Product

Resources

About