Cement-based materials are the most prevalent construction materials, and the conventional cutting techniques are still mostly used for fabricating the materials. However, these conventional cutting methods could generate undesirable micro-cracks and remove unintentional structural sections. This experimental study aims to evaluate the effects of the new fabricating method using laser on the microstructural characteristics of the cement-based materials. The experimental variables are laser cutting speed, water to cement ratio and material compositions. In order to compare the microstructure before and after the laser interaction, the microstructure of the cut surface is observed through scanning electron microscopy/energy dispersive X-Ray (SEM/EDX). After the laser interaction, the Material Removed Zone (MRZ) and Heat Affected Zone (HAZ) are observed on the cut surface. In MRZ, it is found that the glassy layer is thickened by an increasing amount of silicate-based materials in cement-based materials. In addition, it concluded that the amount of silicate-based material mixed in the cement-based materials affects the laser cutting quality.