Special aircraft cabin layout optimization design was treated as an NP-complete (nondeterministic Polynomial time complete) problem. The empirical design was incapable to meet the developing requirement of the display and control system in modern aircraft cabin. After analysis of three typical cabin layout cases, several factors were found to need optimization in these cases. Therefore, the present study proposed an improved genetic algorithm based on human factors. An optimized cabin layout was carried out by setting the objective function and constraints. Besides, aiming at evaluating the cabin layout, JACK 3-D human analysis software was applied to calculate the operator's reach zone and view cone. This study could provide a technical reference for the cabin layout design of special aircraft.