Computational Proteome-Wide Study for the Prediction of Escherichia coli Protein Targeting in Host Cell Organelles and Their Implication in Development of Colon Cancer

Abstract

Enterohemorrhagic Escherichia coli infection is associated with gastrointestinal disorders, including diarrhea and colorectal cancer. Although evidences have established the involvement of E. coli in the growth of colon cancer, the molecular mechanisms of carcinogenesis of cancer growth and development are not well understood. We analyzed E. coli protein targeting in host cell organelles and the implication in colon cancer using in silico approaches. Our results indicated that many E. coli proteins targeted the endoplasmic reticulum (ER), ER membranes, Golgi apparatus, Golgi apparatus membranes, peroxisomes, nucleus, nuclear membrane, mitochondria, and mitochondrial membrane of host cells. These targeted proteins in ER, Golgi apparatus, peroxisomes, nucleus, and mitochondria may alter the normal functioning of various pathways including DNA repair, apoptosis, replication, transcription, and protein folding in E. coli-infected host cells. The results of the current in silico study provide insights into E. coli pathogenesis and may aid in designing new preventive and therapeutic strategies.

Expression and Prognostic Analyses of the Insulin-like Growth Factor 2 mRNA Binding Protein Family in Human Pancreatic Cancer

“…In our enrichment analysis, P53 was also upregulated, consistent with the conclusion of Dianov et al Although the relationship between abnormalities in the BER pathway and the development and prognosis of cancer has been studied [19][20][21], in pancreatic cancer, whether IGF2BP2 is associated with this process has not yet been elucidated. The positive correlation between pathogenic Escherichia coli (E. coli) infection and colon cancer has been con rmed in multiple studies [22,23]. The infection of pathogenic E. coli destroys the microenvironment of the micro ora in the intestinal tract, thereby inducing colon cancer [22,23,24].…”

“…The positive correlation between pathogenic Escherichia coli (E. coli) infection and colon cancer has been con rmed in multiple studies [22,23]. The infection of pathogenic E. coli destroys the microenvironment of the micro ora in the intestinal tract, thereby inducing colon cancer [22,23,24]. In studies of pathogenic Escherichia coli infection-induced pathways for pancreatic cancer, there is a lack of clear evidence that this pathway is associated with pancreatic cancer.…”

Expression and Prognosis Analyses of Insulin-Like Growth Factor 2 mRNA Binding Protein Family in Human Pancreatic Cancer

“…In pancreatic cancer, whether IGF2BP2 is associated with this process has not been elucidated. The positive correlation between pathogenic Escherichia coli (E. coli) infection and colon cancer has been con rmed by multiple studies [22,23]. The infection of pathogenic E. coli destroys the microenvironment of the micro ora in the intestinal tract, thereby inducing colon cancer [22,23,24].…”

“…The positive correlation between pathogenic Escherichia coli (E. coli) infection and colon cancer has been con rmed by multiple studies [22,23]. The infection of pathogenic E. coli destroys the microenvironment of the micro ora in the intestinal tract, thereby inducing colon cancer [22,23,24]. In studies of pathogenic Escherichia coli infection-induced pathways for pancreatic cancer, there was a lack of clear evidence that this pathway was associated with pancreatic cancer.…”

Cytotoxic potentials of silibinin assisted silver nanoparticles on human colorectal HT-29 cancer cells

“…Colorectal cancer (CRC) kills almost 700,000 people annually, making it one of the second leading cause of cancer-related deaths worldwide and it also accounts for approximately 10% of cancerrelated mortality in Western countries [1][2][3]. Various factors have associated with progression of colon cancer including mutation, bacterial infection and irradiation [4][5][6]. The colon cancer incidence is increasing in several countries due to the pervasive adoption of the Western diet and lifestyle [7].…”