Computer-aided design of reversible hybridization chain reaction (CAD-HCR) enables multiplexed single-cell spatial proteomics imaging

Liu, Xiaohao; Mao, Dali; Song, Yuanlin; Zhu, Liucun; Isak, Albertina N; Lu, Cuicui; Deng, Guoli; Chen, Feng; Sun, Fenyong; Yang, Yu; Zhu, Xiaoli; Tan, Weihong

doi:10.1126/sciadv.abk0133

Cited by 29 publications

(21 citation statements)

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“…23,24 Several isothermal amplification techniques such as rolling circle amplification (RCA), exponential amplification reaction (EXPAR), strand displacement amplification (SDA), hybridization chain reaction (HCR), and DNAzyme have been proposed. [25][26][27][28][29] Considering the simple primer design, exponential amplification kinetics, and high amplification efficiency, the EXPAR has attracted considerable attention. 30,31 In the EXPAR, short ssDNA (single-stranded DNA) can trigger a combination of polymerase strand extension and single-strand nicking, so the "extension-cleavagestrand displacement" can be repeated continuously and result in amplification.…”

Section: Introductionmentioning

confidence: 99%

A DNA walker triggered isothermal amplification method based on freezing construction of AuNP probes and its application in ricin detection

Sun

Wang²,

Chai

et al. 2023

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Section: Introductionmentioning

confidence: 99%

A DNA walker triggered isothermal amplification method based on freezing construction of AuNP probes and its application in ricin detection

Sun

Wang²,

Chai

et al. 2023

Analyst

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“…Nanotechnology‐based cancer therapy has already become a hot topic of research and has been recognized recently (Liu et al, 2022; Wang, Zhong, & Cheng, 2021; Yang et al, 2021). Although several reviews have described nanotherapeutics for the treatment of glioblastoma (Hsu et al, 2021; Zhao et al, 2020; Zottel et al, 2019), a systematic review on nanotechnology‐based combination therapy in GBM is lacking.…”

Section: Introductionmentioning

confidence: 99%

Nanotechnology meets glioblastoma multiforme: Emerging therapeutic strategies

Liu

Wang

et al. 2022

WIREs Nanomed Nanobiotechnol

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Glioblastoma multiforme (GBM) represents the most common and fatal form of primary invasive brain tumors as it affects a great number of patients each year and has a median overall survival of approximately 14.6 months after diagnosis. Despite intensive treatment, almost all patients with GBM experience recurrence, and their 5‐year survival rate is approximately 5%. At present, the main clinical treatment strategy includes surgical resection, radiotherapy, and chemotherapy. However, tumor heterogeneity, blood–brain barrier, glioma stem cells, and DNA damage repair mechanisms hinder efficient GBM treatment. The emergence of nanometer‐scale diagnostic and therapeutic approaches in cancer medicine due to the establishment of nanotechnology provides novel and promising tools that will allow us to overcome these difficulties. This review summarizes the application and recent progress in nanotechnology‐based monotherapies (e.g., chemotherapy) and combination cancer treatment strategies (chemotherapy‐based combined cancer therapy) for GBM and describes the synergistic enhancement between these combination therapies as well as the current standard therapy for brain cancer and its deficiencies. These combination therapies that can reduce individual drug‐related toxicities and significantly enhance therapeutic efficiency have recently undergone rapid development. The mechanisms underlying these different nanotechnology‐based therapies as well as the application of nanotechnology in GBM (e.g., in photodynamic therapy and chemodynamic therapy) have been systematically summarized here in an attempt to review recent developments and to identify promising directions for future research. This review provides novel and clinically significant insights and directions for the treatment of GBM, which is of great clinical importance. This article is categorized under: Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease Diagnostic Tools > In Vivo Nanodiagnostics and Imaging

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“…Small molecules or biomolecules, such as antibiotics, have been used in clinics for almost a century. More recently, the development of metagenomic-, metametabolomic-, metaproteomic-, and metatranscriptomicbased technologies has involved material engineering, including surface patterning techniques, fluorescent bead labeling techniques, microfluidic devices, and optical sensing technologies [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Material Engineering in Gut Microbiome and Human Health

et al. 2022

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Tremendous progress has been made in the past decade regarding our understanding of the gut microbiome’s role in human health. Currently, however, a comprehensive and focused review marrying the two distinct fields of gut microbiome and material research is lacking. To bridge the gap, the current paper discusses critical aspects of the rapidly emerging research topic of “material engineering in the gut microbiome and human health.” By engaging scientists with diverse backgrounds in biomaterials, gut-microbiome axis, neuroscience, synthetic biology, tissue engineering, and biosensing in a dialogue, our goal is to accelerate the development of research tools for gut microbiome research and the development of therapeutics that target the gut microbiome. For this purpose, state-of-the-art knowledge is presented here on biomaterial technologies that facilitate the study, analysis, and manipulation of the gut microbiome, including intestinal organoids, gut-on-chip models, hydrogels for spatial mapping of gut microbiome compositions, microbiome biosensors, and oral bacteria delivery systems. In addition, a discussion is provided regarding the microbiome-gut-brain axis and the critical roles that biomaterials can play to investigate and regulate the axis. Lastly, perspectives are provided regarding future directions on how to develop and use novel biomaterials in gut microbiome research, as well as essential regulatory rules in clinical translation. In this way, we hope to inspire research into future biomaterial technologies to advance gut microbiome research and gut microbiome-based theragnostics.

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Computer-aided design of reversible hybridization chain reaction (CAD-HCR) enables multiplexed single-cell spatial proteomics imaging

Abstract: Cyclic imaging of multiple proteins in a single cell is realized through CAD-HCR.

Cited by 29 publications

References 50 publications

A DNA walker triggered isothermal amplification method based on freezing construction of AuNP probes and its application in ricin detection

A DNA walker triggered isothermal amplification method based on freezing construction of AuNP probes and its application in ricin detection

Nanotechnology meets glioblastoma multiforme: Emerging therapeutic strategies

Material Engineering in Gut Microbiome and Human Health

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