Computer‐assisted, high‐performance liquid chromatography with mass spectrometric detection for the analysis of coumarins in Peucedanum palustre and Angelica archangelica

Abstract

A reversed-phase HPLC method with atmospheric pressure chemical ionisation MS detection has been developed for the separation and identification of coumarins in plants of Peucedanum palustre L. (Moench) and Angelica archangelica (L.) var. archangelica. The Turbo Method Development program was utilised to optimise the mobile phase with two organic solvents (acetonitrile and methanol) and two aqueous solutions (1.0% formic acid and 10 mM ammonium acetate). Optimisation of the solvent gradients for the method was performed with the aid of the DryLab program. Analyses were carried out using a Phenomenex Prodigy RP C18 column. Fifty-two peaks (14 of which were associated with coumarins) were separated in 30 min from extracts of P. palustre, and 48 peaks (15 associated with coumarins) from extracts of A. archangelica. A total of 21 different coumarin-type compounds were identified in the aerial and the underground parts of the title plants. Isopimpinellin and pimpinellin were found for the first time in P. palustre and were identified by comparison of retention times and MS data obtained following the analysis of pure standards. This is the first report of the coumarin composition of the umbels of P. palustre.

Fingerprint analysis of the fruits of Cnidium monnieri extract by high-performance liquid chromatography–diode array detection–electrospray ionization tandem mass spectrometry

“…Then the other tentatively identified coumarins and chromones in the fingerprint chromatogram can assist to identify the plant species. The compounds in other related plants are shown in Table 2 [35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53].…”

Profiling of Coumarins in Peucedanum palustre (L.) Moench Populations Growing in Finland

“…It grows in moist inland environments, e.g ., on river banks or in moist forests . The plant contains a complex mixture of coumarin‐structured compounds, i.e ., simple coumarins, and linear and angular furano‐ and hydroxyisopropylfuranocoumarins as well as volatile oil . Coumarins are known to have several roles in the plant, especially in the defense against insects and plant pathogens, e.g ., , but they may also act as attractants to certain insect species .…”

Furanocoumarins

“…Ammi majus [24] Angelica archangelica [300] Heracleum laciniatum [273] Pimpinella saxifraga [301] Isobergaptol …”