Computer-assisted infrared thermographic study of axon reflex induced by intradermal melittin

Koyama, Natsu; Hirata, Kazuhiko; Hori, Koichiro; Dan, Kenjiro; Yokota, Toshikatsu

doi:10.1016/s0304-3959(99)00192-x

Cited by 33 publications

(22 citation statements)

References 24 publications

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“…This result was not likely due to systemic effects of the two drugs since local injection of the two antagonists in the non-injected paw did not have any significantly pharmacological influence upon melittin-induced nociception and hypersensitivity. Human experiments showed that intradermal injection of melittin could elicit severe spontaneous pain, followed by sustained increase in skin temperature and a process of neurogenic inflammation (visual flare) accompanied by heat or mechanical hyperalgesia [20][21][22][23]. Similar results have also been obtained from our research in rats [11,12].…”

Section: Discussionsupporting

confidence: 88%

Roles of Peripheral P2X and P2Y Receptors in the Development of Melittin-Induced Nociception and Hypersensitivity

Xie

Han

et al. 2008

Neurochem Res

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A recent report from our laboratory shows that subcutaneous (s.c.) injection of melittin could induce persistent spontaneous nociception (PSN) and primary thermal or mechanical hyperalgesia. However, the exact peripheral mechanisms underlying melittin-induced multiple pain-related behaviors remain unclear. In this study, behavioral tests combined with pharmacological manipulations were used to explore potential roles of local P2X and P2Y receptors in melittin-induced inflammatory pain and hyperalgesia. Post-treatment of the primary injury site with s.c. injection of A-317491 (a potent P2X(3)/P2X(2/3) receptor antagonist) and Reactive Blue 2 (a potent P2Y receptor antagonist) could significantly suppress the development of melittin-evoked PSN and hypersensitivity (thermal and mechanical). Our control experiments demonstrated that local administration of either antagonist into the contralateral hindpaw produced no significant effect on any kind of pain-associated behaviors. Taken together, these data indicate that activation of P2X and P2Y receptors might be essential to the maintenance of melittin-induced primary thermal and mechanical hyperalgesia as well as on-going pain.

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Section: Discussionsupporting

confidence: 88%

Roles of Peripheral P2X and P2Y Receptors in the Development of Melittin-Induced Nociception and Hypersensitivity

Xie

Han

et al. 2008

Neurochem Res

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“…To date, the mean areas (mm 2 ) of temperature above basal peak temperature, e.g. in ranges of 0.2°C, have usually been calculated (Koyama et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Computer-assisted skin videothermography is a highly sensitive quality tool in the diagnosis and monitoring of complex regional pain syndrome type�I

Huygen

Niehof

Klein

et al. 2004

European Journal of Applied Physiology

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The use of thermography in the diagnosis and evaluation of complex regional pain syndrome type 1 (CRPS1) is based on the presence of temperature asymmetries between the involved area of the extremity and the corresponding area of the uninvolved extremity. The interpretation of thermographic images is, however, subjective and not validated for routine use. The objective of the present study was to develop a sensitive, specific and reproducible arithmetical model as the result of computer-assisted infrared thermography in patients with early stage CRPS1 in one hand. Eighteen patients with CRPS1 on one hand and 13 healthy volunteers were included in the study. The severity of the disease was determined by means of pain questionnaires [visual analogue scale (VAS) pain and McGill Pain Questionnaire], measurements of mobility (active range of motion) and oedema volume. Asymmetry between the involved and the uninvolved extremities was calculated by means of the asymmetry factor, the ratio and the average temperature differences. The discrimination power of the three methods was determined by the receiver-operating curve (ROC). The regression between the determined temperature distributions of both extremities was plotted. Subsequently the correlation of the data was calculated. In normal healthy individuals the asymmetry factor was 0.91 (0.01) (SD), whereas in CRPS1 patients this factor was 0.45 (0.07) (SD). The performance of the arithmetic model based on the ROC curve was excellent. The area under the curve was 0.97, the P value was <0.001, the sensitivity 92% and specificity 94%. Furthermore, the temperature asymmetry factor was correlated with the duration of the disease and VAS pain. In conclusion, in resting condition, videothermography is a reliable additive diagnostic tool of early stage CRPS1. This objective tool could be used for monitoring purposes during experimental therapeutic intervention.

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“…However, due to increased interest in animal studies on bee venom-induced nociception or pain, the first experimental study on pain and inflammatory responses of seven healthy adults (2 women and 5 men) to intradermal (i.d.) injection of melittin was carefully conducted and published in 2000 (Koyama et al, 2000). The human subjects reported the pain intensity experienced using a 0–10 visual analog scale (VAS) in which scores of 0, 5 and 10 indicated “no pain”, “moderate pain” and “intolerable pain”, respectively.…”

Section: Nociceptive and Inflammatory Effects Of Subcutaneous Bee mentioning

confidence: 99%

The nociceptive and anti-nociceptive effects of bee venom injection and therapy: A double-edged sword

Chen

Lariviere

2010

Progress in Neurobiology

166

121

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Bee venom injection as a therapy, like many other complementary and alternative medicine approaches, has been used for thousands of years to attempt to alleviate a range of diseases including arthritis. More recently, additional theraupeutic goals have been added to the list of diseases making this a critical time to evaluate the evidence for the beneficial and adverse effects of bee venom injection. Although reports of pain reduction (analgesic and antinociceptive) and anti-inflammatory effects of bee venom injection are accumulating in the literature, it is common knowledge that bee venom stings are painful and produce inflammation. In addition, a significant number of studies have been performed in the past decade highlighting that injection of bee venom and components of bee venom produce significant signs of pain or nociception, inflammation and many effects at multiple levels of immediate, acute and prolonged pain processes. This report reviews the extensive new data regarding the deleterious effects of bee venom injection in people and animals, our current understanding of the responsible underlying mechanisms and critical venom components, and provides a critical evaluation of reports of the beneficial effects of bee venom injection in people and animals and the proposed underlying mechanisms. Although further studies are required to make firm conclusions, therapeutic bee venom injection may be beneficial for some patients, but may also be harmful. This report highlights key patterns of results, critical shortcomings, and essential areas requiring further study.

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Computer-assisted infrared thermographic study of axon reflex induced by intradermal melittin

Cited by 33 publications

References 24 publications

Roles of Peripheral P2X and P2Y Receptors in the Development of Melittin-Induced Nociception and Hypersensitivity

Roles of Peripheral P2X and P2Y Receptors in the Development of Melittin-Induced Nociception and Hypersensitivity

Computer-assisted skin videothermography is a highly sensitive quality tool in the diagnosis and monitoring of complex regional pain syndrome type�I

The nociceptive and anti-nociceptive effects of bee venom injection and therapy: A double-edged sword

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