Computer-assisted transfer of programmed elutions in reversed-phase high-performance liquid chromatography

Systematic, computer-assisted development of high performance liquid chromatography for multi-component analysis

“…All chromatographic experiments were performed in the linear gradient mode, which is the easiest choice and the most commonly used in analytical laboratories. 16 According to several pre-experiments, acetonitrile (phase A) and formic acid solution (phase B) were chosen as the mobile phase. The formic acid was determined as the aqueous component, which was applicable to the ELSD detector and optimized the performance of the chromatographic peaks.…”

Reversed-phase liquid chromatographic method with spectrophotometric detection for the determination of antiretroviral drugs

“…Chromatographic conditions have been thoroughly optimized using experimental design and multicriteria functions for reaching a suitable compromise regarding resolution of close peaks and analysis time. Similar approaches have been considered elsewhere for facilitating the optimization of pretreatment and separation conditions and extracting relevant information from a limited number of experiments [27][28][29][30].…”

A Binary-Like Approach for the Computer Assisted Method Development of Isocratic and Programmed Ternary Solvent Elutions in Reversed-Phase Liquid Chromatography

“…Optimization processes were developed with evolutionary algorithms that utilize both conventional critical resolution and chromatographic response functions (CRFs) as objective functions in the search for the optimum. Pareto optimality (15) and transfer routines (16) between instruments have also been implemented as options. All PREGA modules, including the specific module for exploring ternary solvent mixtures presented here can be downloaded free at http://www.usc.es/gcqprega.…”