Concanavalin A and the motile behaviour of the ciliate Tetrahymena

Leick, Vagn; Iversen, Charlotte Goos; Olesen, Jesper; Bøg-Hansen, T.C.

doi:10.1078/0932-4739-00009

Cited by 5 publications

(4 citation statements)

References 14 publications

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“…Relatively little is known about chemotaxis to sugars compared with chemotaxis to peptides by nonrumen ciliates. Sugar-binding sites on cellular membranes might be relatively nonspecific in Tetrahymena (Leick et al, 2003). The potential for mannose to block chemotaxis in that study and with planktonic protists (Wootton et al, 2007) and the observation that mannose is toxic to rumen isotrichids (Williams and Coleman, 1992) deserves further attention.…”

Section: Experiments 2: Comparison Of Glucose and Xylose As Chemoattractantsmentioning

confidence: 83%

Chemotaxis toward carbohydrates and peptides by mixed ruminal protozoa when fed, fasted, or incubated with polyunsaturated fatty acids

Diaz

Karnati

Lyons

et al. 2014

Journal of Dairy Science

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In contrast to the well-characterized chemotaxis and migratory behavior between the dorsal and ventral locations of the rumen by isotrichids, we hypothesized that chemotaxis toward soluble nutrients maintains entodiniomorphid protozoa in the particulate fraction. The objectives of these experiments were to compare the dose-responsive chemotaxis (1) toward different glucose concentrations when ruminal samples were harvested from fed versus fasted cows; (2) toward increasing concentrations of glucose compared with xylose when protozoa were harvested from a fed cow; (3) toward peptides of bacterial, protozoal, and soy origin; and (4) toward glucose when mixed ruminal protozoa were previously incubated for 0, 3, or 6h in the presence of emulsified polyunsaturated fatty acids (PUFA; Liposyn II, Hospira, Lake Forest, IL). In experiment 1, isotrichid protozoa decreased chemotaxis toward increasing glucose concentration when cows were fasted. Entodiniomorphids exhibited chemotaxis to similar concentrations of glucose as did isotrichids, but to a lesser magnitude of response. In experiment 2, xylose was chemotactic to both groups. Xylose might draw fibrolytic entodiniomorphid protozoa toward newly ingested feed. In contrast, even though isotrichids should not use xylose as an energy source, they were highly chemoattracted to xylose. In experiment 3, entodiniomorphids were not selectively chemoattracted toward bacterial or protozoal peptides compared with soy peptides. In experiment 4, despite isotrichid populations decreasing in abundance with increasing time of incubation in PUFA, chemotaxis to glucose remained unchanged. In contrast, entodiniomorphids recovered chemotaxis to glucose with increased time of PUFA incubation. Current results support isotrichid chemotaxis to sugars but also our hypothesis that a more moderate chemotaxis toward glucose and peptides explains how they swim in the fluid but pass from the rumen with the potentially digestible fraction of particulates.

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Section: Experiments 2: Comparison Of Glucose and Xylose As Chemoattractantsmentioning

confidence: 83%

Chemotaxis toward carbohydrates and peptides by mixed ruminal protozoa when fed, fasted, or incubated with polyunsaturated fatty acids

Diaz

Karnati

Lyons

et al. 2014

Journal of Dairy Science

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“…Tetrahymena malaccensis) express identical behavioural patterns in trajectory, reorientation, sliding etc. 44 The above mentioned data explains also the fact that swimming behaviour has a close relationship to the dynamics of surface membrane receptors of Terahymena and the special molecular level " memory" of these cells developed by pretreatments -imprinting -can also modify quality of ethograms for generations. 43 The good matching between chemotaxis and the swimming velocities/tortuosity of the swimming paths underline the significance of our observation that even in sugars these migratory properties are closely related.…”

Section: Accepted Manuscriptmentioning

confidence: 83%

Chemotactic effect of mono- and disaccharides on the unicellular Tetrahymena pyriformis

Szemes

Lajkó

Láng

et al. 2015

Carbohydrate Research

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“…Among them, cell locomotion is easy to monitor to directly manifest the ciliary motility. Previous studies demonstrated the correlation between cell movement and exposure to carbon nanotubes, metals, and pesticides in ciliates [ 31 , 32 , 33 , 34 ]. For example, a high concentration of lanthanum ions causes a decrease in cell motility and the rocking movement [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Establishing a High-Throughput Locomotion Tracking Method for Multiple Biological Assessments in Tetrahymena

Suryanto

Vasquez

Roldan

et al. 2022

Cells

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Protozoa are eukaryotic, unicellular microorganisms that have an important ecological role, are easy to handle, and grow rapidly, which makes them suitable for ecotoxicity assessment. Previous methods for locomotion tracking in protozoa are largely based on software with the drawback of high cost and/or low operation throughput. This study aimed to develop an automated pipeline to measure the locomotion activity of the ciliated protozoan Tetrahymena thermophila using a machine learning-based software, TRex, to conduct tracking. Behavioral endpoints, including the total distance, velocity, burst movement, angular velocity, meandering, and rotation movement, were derived from the coordinates of individual cells. To validate the utility, we measured the locomotor activity in either the knockout mutant of the dynein subunit DYH7 or under starvation. Significant reduction of locomotion and alteration of behavior was detected in either the dynein mutant or in the starvation condition. We also analyzed how Tetrahymena locomotion was affected by the exposure to copper sulfate and showed that our method indeed can be used to conduct a toxicity assessment in a high-throughput manner. Finally, we performed a principal component analysis and hierarchy clustering to demonstrate that our analysis could potentially differentiate altered behaviors affected by different factors. Taken together, this study offers a robust methodology for Tetrahymena locomotion tracking in a high-throughput manner for the first time.

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Concanavalin A and the motile behaviour of the ciliate Tetrahymena

Cited by 5 publications

References 14 publications

Chemotaxis toward carbohydrates and peptides by mixed ruminal protozoa when fed, fasted, or incubated with polyunsaturated fatty acids

Chemotaxis toward carbohydrates and peptides by mixed ruminal protozoa when fed, fasted, or incubated with polyunsaturated fatty acids

Chemotactic effect of mono- and disaccharides on the unicellular Tetrahymena pyriformis

Establishing a High-Throughput Locomotion Tracking Method for Multiple Biological Assessments in Tetrahymena

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