Concanavalin A for in vivo glucose sensing: A biotoxicity review

Ballerstädt, Ralph; Evans, Colton; McNichols, Roger J.; Gowda, Ashok

doi:10.1016/j.bios.2006.01.008

Cited by 133 publications

(99 citation statements)

References 84 publications

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“…The dose used in our study has been shown to be safe and no signs of hepatic, hematologic, or any overt toxicity were reported. 55 The interval between treatments was chosen based on the preliminary studies utilizing fluorescently labeled ConA, which was retained in the renal microvasculature for at least 4 days. In the assessment of aortic matrilytic activity, aortic rings were cultured in 3D matrigel for up to 9 days.…”

Section: In Vivo Induction Of Mmp-14 With Conamentioning

confidence: 99%

Endothelial Sirtuin 1 Deficiency Perpetrates Nephrosclerosis through Downregulation of Matrix Metalloproteinase-14

Vasko

Xavier

Lin

et al. 2014

Journal of the American Society of Nephrology

105

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Sirtuin 1 (SIRT1) depletion in vascular endothelial cells mediates endothelial dysfunction and premature senescence in diverse cardiovascular and renal diseases. However, the molecular mechanisms underlying these pathologic effects remain unclear. Here, we examined the phenotype of a mouse model of vascular senescence created by genetically ablating exon 4 of Sirt1 in endothelial cells (Sirt1 endo2/2 ). Under basal conditions, Sirt1 endo2/2 mice showed impaired endothelium-dependent vasorelaxation and angiogenesis, and fibrosis occurred spontaneously at low levels at an early age. In contrast, induction of nephrotoxic stress (acute and chronic folic acid-induced nephropathy) in Sirt1 endo2/2 mice resulted in robust acute renal functional deterioration followed by an exaggerated fibrotic response compared with control animals. Additional studies identified matrix metalloproteinase-14 (MMP-14) as a target of SIRT1. In the kidneys of Sirt1 endo2/2 mice, impaired angiogenesis, reduced matrilytic activity, and retention of the profibrotic cleavage substrates tissue transglutaminase and endoglin accompanied MMP-14 suppression. Furthermore, restoration of MMP-14 expression in SIRT1-depeleted mice improved angiogenic and matrilytic functions of the endothelium, prevented renal dysfunction, and attenuated nephrosclerosis. Our findings establish a novel mechanistic molecular link between endothelial SIRT1 depletion, downregulation of MMP-14, and the development of nephrosclerosis.

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Section: In Vivo Induction Of Mmp-14 With Conamentioning

confidence: 99%

Endothelial Sirtuin 1 Deficiency Perpetrates Nephrosclerosis through Downregulation of Matrix Metalloproteinase-14

Vasko

Xavier

Lin

et al. 2014

Journal of the American Society of Nephrology

105

View full text Add to dashboard Cite

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“…Existing methods of fluorescent glucose sensing apply fluorescence resonance energy transfer (FRET). 45 This method is based on the dual measure, i.e., the FRET and fluorescence intensity (I) measurements. FRET is a distance-dependent energy transfer from a fluorophore donor (D) to a fluorophore acceptor (A) in a nonradiative process.…”

Section: Contact Lens Sensor For Diabetesmentioning

confidence: 99%

Noninvasive Diagnostic Devices for Diabetes through Measuring Tear Glucose

Zhang

Hodge

Hutnick

et al. 2011

J Diabetes Sci Technol

137

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This article reviews the development of a noninvasive diagnostic for diabetes by detecting ocular glucose. Early diagnosis and daily management are very important to diabetes patients to ensure a healthy life. Commercial blood glucose sensors have been used since the 1970s. Millions of diabetes patients have to prick their finger for a drop of blood 4-5 times a day to check blood glucose levels-almost 1800 times annually. There is a strong need to have a noninvasive device to help patients to manage the disease easily and painlessly. Instead of detecting the glucose in blood, monitoring the glucose level in other body fluids may provide a feasible approach for noninvasive diagnosis and diabetes control. Tear glucose has been studied for several decades. This article reviews studies on ocular glucose and its monitoring methods. Attempts to continuously monitor the concentration of tear glucose by using contact lens-based sensors are discussed as well as our current development of a nanostructured lens-based sensor for diabetes. This disposable biosensor for the detection of tear glucose may provide an alternative method to help patients manage the disease conveniently.

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“…[ [14,[16][17][18]] that bind to carbohydrate moieties on the cell surface, but especially in the latter case the cell-binding molecules themselves have been reported to have a degree of cytotoxicity that can influence the cellular properties being evaluated. [17,[20][21][22] Thus, while these studies highlight the utility of AFM for the measurement of cell receptor-ligand interactions, an expanded set of cantilever attachment methods will be needed for the study of cell-cell interactions over widely varying time scales.To address this need, we have compared three biomolecule-mediated methods for the attachment of live cells to AFM cantilevers, with an emphasis on the cell viability, adhesion strength, and probe reuse that each technique can achieve. These studies have indicated that cell attachment through the use of complementary DNA strands has the least influence on viability and does not appear to activate cell signaling pathways.…”

mentioning

confidence: 99%

“…[13][14][15][16][17][18][19] Several fundamental adhesion measurements have been achieved by coating AFM cantilevers with fibronectin [19] or lectins [14,[16][17][18] that bind to carbohydrate moieties on the cell surface, but especially in the latter case the cell-binding molecules themselves have been reported to have a degree of cytotoxicity that can influence the cellular properties being evaluated. [17,[20][21][22] Thus, while these studies highlight the utility of AFM for the measurement of cell receptor-ligand interactions, an expanded set of cantilever attachment methods will be needed for the study of cell-cell interactions over widely varying time scales.…”

mentioning

confidence: 99%

DNA‐Coated AFM Cantilevers for the Investigation of Cell Adhesion and the Patterning of Live Cells

et al. 2008

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Measurement of receptor adhesion strength requires the precise manipulation of single cells on a contact surface. To attach live cells to a moveable probe, DNA sequences complementary to strands displayed on the plasma membrane are introduced onto AFM cantilevers (see picture, bp=base pairs). The strength of the resulting linkages can be tuned by varying the length of DNA strands, allowing for controlled transport of the cells Keywords AFM; cell adhesion; DNA; metabolic engineering; patterningThe forces governing cell-cell adhesion are vitally important to many biological processes, including cell differentiation, tissue growth, [1,2] tumorigenesis, [1,3] and proper functioning of the vertebrate immune response. [4,5] The strengths of these interactions are typically characterized through the attachment of single living cells to probes that are capable of force measurement, such as suction micropipettes. [6,7] More recently, optical tweezers [8,9] have been applied to capture single cells and to measure these forces with high accuracy, but this technique is limited to applying forces in the piconewton range. [ [14,[16][17][18]] that bind to carbohydrate moieties on the cell surface, but especially in the latter case the cell-binding molecules themselves have been reported to have a degree of cytotoxicity that can influence the cellular properties being evaluated. [17,[20][21][22] Thus, while these studies highlight the utility of AFM for the measurement of cell receptor-ligand interactions, an expanded set of cantilever attachment methods will be needed for the study of cell-cell interactions over widely varying time scales.To address this need, we have compared three biomolecule-mediated methods for the attachment of live cells to AFM cantilevers, with an emphasis on the cell viability, adhesion strength, and probe reuse that each technique can achieve. These studies have indicated that cell attachment through the use of complementary DNA strands has the least influence on viability and does not appear to activate cell signaling pathways. This method also offers overall superior adhesion strength, but this parameter can be attenuated to allow cells to be transferred from one surface to another. We were able to demonstrate this concept by picking up free cells and placing them in exact positions on a substrate bearing DNA strands with longer complementary regions. This "dippen" [23][24][25] live-cell patterning demonstrates the reusability of the DNA-mediated cell adhesion method and could prove useful for the construction of complex mixtures of cells with well-defined spatial relationships.To allow the comparison of several attachment strategies, three different biomolecules (DNA, concanavalin A (ConA), and an antibody) were attached to silicon nitride AFM cantilevers for cell anchoring. For all attachment methods, the thin layer of silicon oxide on the working surface was covered with aldehyde groups as outlined in Figure 1 a. The surfaces produced using these steps were characterized by contact-angle...

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Concanavalin A for in vivo glucose sensing: A biotoxicity review

Cited by 133 publications

References 84 publications

Endothelial Sirtuin 1 Deficiency Perpetrates Nephrosclerosis through Downregulation of Matrix Metalloproteinase-14

Endothelial Sirtuin 1 Deficiency Perpetrates Nephrosclerosis through Downregulation of Matrix Metalloproteinase-14

Noninvasive Diagnostic Devices for Diabetes through Measuring Tear Glucose

DNA‐Coated AFM Cantilevers for the Investigation of Cell Adhesion and the Patterning of Live Cells

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