Concentrações de BAP sobre a proliferação in vitro de brotos de Lippia alba [(Mill.)N.E.Brown]

Abstract

Lippia alba é uma planta de origem brasileira, pertencente à família Verbenaceae. É conhecida por combater a insônia e a asma, além de possuir ação anticonvulsivante e antifúngica. A micropropagação tem sido utilizada para multiplicação de várias espécies com propriedades medicinais, e o BAP é a citocinina sintética mais utilizada para a multiplicação in vitro. O objetivo deste trabalho foi estabelecer a concentração adequada de BAP na multiplicação in vitro de L. alba. Explantes constituídos de segmentos nodais provenientes de plântulas já estabelecidas in vitro com aproximadamente 0,5 cm foram inoculados em meio MS, suplementado com 0,0; 0,5; 1,0 e 1,5 mg L-1 de BAP em adição de 30 g L-1 de sacarose. A utilização de 1,5 mg L-1 de BAP promove a multiplicação in vitro de L. alba. A ausência deste regulador propicia maior número de folhas e o uso na concentração de 0,5 mg L-1 aumentou as massas fresca e seca de parte aérea nesta espécie.

In vitro propagation and acclimatization of genipapo accessions

“…Similar results were observed in blackberry plants, with maximum leaf development (7.89) in the presence of 1.0 mg L -1 BAP (Villa et al, 2005), and in Dendrobium nobile seedlings, with the greatest number of leaves (5.47) in the presence of 2.7 mg L -1 BAP (Soares et al, 2011). These results disagree with those recorded in nodal segments of Lipia alba, where the greatest number of leaves was observed in the absence of BAP (Asmar et al, 2012). According to the authors, this cytokinin promotes a greater number of small-sized buds, showing a smaller number of nodal segments and leaves, an effect that was not observed in this study.…”

Genome Status of Lippia alba Polyploid Complex Long-term in vitro Cultivated

“…In general, the in vitro plant regeneration procedure involved the use of growth regulators even when axillary buds were used as explants [19,20,48,49,50]. In L. alba, for example, the cytokinin BAP has been used in the cultivation of nodal segments with the purpose of rapid multiplication [39,40,41]. We demonstrated that L. alba does not require supplemental growth regulators for in vitro maintenance as was reported for other species such as plant regeneration procedure involved the use of growth regulators even when axillary buds were used as explants , for example, the cytokinin BAP has been used in the cultivation of nodal segments with the purpose of rapid iplication [39,40,41].…”

“…In L. alba, for example, the cytokinin BAP has been used in the cultivation of nodal segments with the purpose of rapid multiplication [39,40,41]. We demonstrated that L. alba does not require supplemental growth regulators for in vitro maintenance as was reported for other species such as plant regeneration procedure involved the use of growth regulators even when axillary buds were used as explants , for example, the cytokinin BAP has been used in the cultivation of nodal segments with the purpose of rapid iplication [39,40,41]. We demonstrated that does not require supplemental growth maintenance as was reported for other species such as Bacopa monnieri [14] and Scutellaria baicalensis The authors demonstrated the propagation of axillary buds on hormone-free MS medium.…”

“…In spite of the importance of the species, few studies have investigated aspects of the in vitro cultivation of L. alba, focusing mainly in the plant regeneration response either from the apical/axillary buds [24,39,40,41,42,43] or leafderived calli [44]. To date, no study on L. alba in vitro cultivation has assessed the genetic fidelity of long-term micropropagated plants.…”