Concentration-dependent differential effects of N-acetyl-l-cysteine on the expression of HSP70 and metallothionein genes induced by cadmium in human amniotic cells

Small Molecule Activators of the Heat Shock Response: Chemical Properties, Molecular Targets, and Therapeutic Promise

“…75,87 These molecules typically are slower to activate the HSR compared with other thiol-reactive compounds or acute heat stress, 33,88 which may be because they require increased time to cross the cell membrane through a transporter and/or that they react more slowly with protein targets responsible for bringing about the response. Nonetheless, increased levels of metal chelators (e.g., GSH, N -acetyl Cys) decrease overall Hsp expression by these molecules, 89–91 suggesting that their reaction with protein targets is required for HSR activation.…”

Distribution of cadmium and metallothionein in CdCl2‐exposed rat kidney: Relationship with apoptosis and regeneration

“…22 After agarose gel electrophoresis (6% formaldehyde), which employed 10 µg total RNA per lane, the RNA were transferred onto a nitrocellulose membrane and hybridized with a 32 P[γ-ATP]-labeled MT-II cDNA probe, as has been previously reported. 23 The radioactivity of the corresponding bands was visualized and measured by an image analyzer (BAS 2000; Fuji, Tokyo, Japan).…”

Differential expression of two stress-inducible hsp70 genes by various stressors

“…Whereas HSC70 is constitutively expressed, HSP70 can be induced by various stresses such as hyperthermia, oxidative stress, heavy metals and amino acid analogues (Morimoto et al, 1992;Abe et al, 1998;Fukamachi et al, 1998;Wagner et al, 1999). HSP70 has an important function as a molecular chaperone for the folding, transport and assembly of newly synthesized polypeptides (Hartl, 1996).…”