Differential expression of two stress-inducible hsp70 genes by various stressors

Lee, Jae‐Seon; Seo, Jong-Soo

doi:10.1038/emm.2002.19

Cited by 36 publications

(29 citation statements)

References 35 publications

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“…Hsp70 has been served as a biomonitoring marker for ecotoxicological studies (Pyza et al, 1997). A strong induction of Hsp 70 was observed under exposure of pollutants, metals and various stresses (Urani et al, 2001;Lee et al, 2002;Sofia et al, 2002) in Baikalian sponges, HepG2 cell lines and murine embrio fibroblast cells, respectively. However, HDJ2 protein was up-regulated only in automobile emission inspectors.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of biological monitoring markers using genomic and proteomic analysis for automobile emission inspectors and waste incinerating workers exposed to polycyclic aromatic hydrocarbons or 2,3,7,8,-tetracholrodedibenzo-p-dioxins

Kim

Oh²,

Lee³

et al. 2004

Exp Mol Med

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Section: Discussionmentioning

confidence: 99%

Evaluation of biological monitoring markers using genomic and proteomic analysis for automobile emission inspectors and waste incinerating workers exposed to polycyclic aromatic hydrocarbons or 2,3,7,8,-tetracholrodedibenzo-p-dioxins

Kim

Oh²,

Lee³

et al. 2004

Exp Mol Med

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“…While many types of stress such as heat shock, heavy metals, and amino acids analogues induce both hsp70.1 and hsp70.3 simultaneously, hypertonic stress has been found to induce hsp70.1 selectively (Tanaka et al, 1988;Lee and Seo, 2002;Woo et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

The role of tonicity responsive enhancer sites in the transcriptional regulation of human hsp70-2 in response to hypertonic stress

Heo

Lee²,

Kim³

et al. 2006

Exp Mol Med

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The inducible 70 kDa heat shock proteins (Hsp70) in mice are encoded by two almost identical genes, hsp70.1 and hsp70.3. Studies have found that only hsp70.1 is induced by hypertonic stress while both hsp70.1 and hsp70.3 genes are expressed in response to heat shock stress. It is unclear if the human counterparts, hsp70-2 and hsp70-1, are differentially regulated by heat shock and osmotic stress. This study found that only hsp70-2 was induced by hypertonic stress in human embryonic kidney epithelial cells and fibroblasts, while heat shock stress induced both hsp70-1 and hsp70-2. The human hsp70-2 promoter region contains three TonE (tonicity-responsive enhancer) sites, which were reported to play an important role in the response to hypertonicity. When the reporter plasmids containing different parts of the 5' flanking region of hsp70-2 were transfected into human embryonic kidney epithelial cells or fibroblasts, one TonE site at -135 was found to play a key role in the response to hypertonicity. The inactivation of the TonE site using site-directed mutagenesis led to the complete loss of induction by hypertonicity, which demonstrates the essential role of the TonE site. This suggests that the TonE site and the TonEBP (TonE binding protein) are the major regulators for the cellular response against high osmolarity in human kidney tissue.

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“…No induction is observed after oxidative stress. [10][11][12] hsp70.1 is constitutively transcribed from one-cell stage to blastocyst stage, 13 whereas hsp70.3 is constitutively expressed from two-cell stage. 14 By the blastocyst stage, the expression of the two genes becomes heat inducible.…”

Section: Introductionmentioning

confidence: 99%

Stress response in mesoangioblast stem cells

Geraci

Turturici

Galli³

et al. 2005

Cell Death Differ

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Stem cells are presumed to survive various stresses, since they are recruited to areas of tissue damage and regeneration, where inflammatory cytokines and cytotoxic cells may result in severe cell injury. We explored the ability of mesoangioblasts to respond to different cell stresses such as heat, heavy metals and osmotic stress, by analyzing heat shock protein (HSP)70 synthesis as a stress indicator. We found that the A6 mesoangioblast stem cells constitutively synthesize HSP70 in a heat shock transcription factor (HSF)-independent way. However, A6 respond to heat shock and cadmium treatment by synthesizing HSP70 over the constitutive expression and this synthesis is HSF1 dependent. The exposure of A6 to copper or to a hypertonic medium does neither induce HSP70 synthesis nor activation of HSF1, while a constitutive binding of constitutive heat shock element binding factor was found. Together, these data suggest that mesoangioblasts constitutively express HSP70 as an 'a priori ' activation mechanism, while they maintain the ability to respond to stress stimuli.

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Differential expression of two stress-inducible hsp70 genes by various stressors

Cited by 36 publications

References 35 publications

Evaluation of biological monitoring markers using genomic and proteomic analysis for automobile emission inspectors and waste incinerating workers exposed to polycyclic aromatic hydrocarbons or 2,3,7,8,-tetracholrodedibenzo-p-dioxins

Evaluation of biological monitoring markers using genomic and proteomic analysis for automobile emission inspectors and waste incinerating workers exposed to polycyclic aromatic hydrocarbons or 2,3,7,8,-tetracholrodedibenzo-p-dioxins

The role of tonicity responsive enhancer sites in the transcriptional regulation of human hsp70-2 in response to hypertonic stress

Stress response in mesoangioblast stem cells

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