Stress response in mesoangioblast stem cells

Geraci, Fabiana; Turturici, Giuseppina; Galli, Daniela; Cossu, Giulio; Giudice, Giovanni; Sconzo, Gabriella

doi:10.1038/sj.cdd.4401794

Cited by 14 publications

(25 citation statements)

References 31 publications

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“…They are embryo vessel-derived stem cells which can be propagated in vitro and can be differentiate into different mesoderm derived cell types (Minasi et al 2002). We have recently studied the stress status of these stem cells in vitro and we have found that they express the inducible form of Hsp70 without any stress independently of HSF1 (Geraci et al 2006). Differences in function between Hsp70 and Hsc70 may be reflected in differences in their intracellular localization before and after heat stress.…”

Section: Discussionmentioning

confidence: 99%

“…Total protein extracts were prepared as previously described (Geraci et al 2006). For subcellular extracts trypsinized cells were harvested and washed in PBS.…”

Section: Preparation Of Total and Subcellular Extractsmentioning

confidence: 99%

“…Our previous data demonstrated that even if A6 stem cells synthesize Hsp70 without any stress in an HSF1 independent way, they are able to respond to cellular stresses by increasing its level (Geraci et al 2006). In order to evaluate whether or not Hsp70 distribution was modified after heat treatment, double fluorescence assays have been performed.…”

Section: Hsp70 Localization In Heat-shocked A6 Cellsmentioning

confidence: 99%

“…Recently it has been demonstrated that glial cells release Hsp70 making neurons, which are exposed to the protein more resistant to cell stress (Guzhova et al 2001;Tytell 2005). The A6 cells, a clone of mouse mesoangioblasts, synthesize the Hsp70 under physiological growth conditions and this synthesis is independent from HSF1 or HSF2, but its role is not yet defined (Geraci et al 2006). To date not many works have studied Hsp70 localization in mammalian cell lines under physiological growth conditions.…”

Section: Introductionmentioning

confidence: 99%

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Hsp70 localizes differently from chaperone Hsc70 in mouse mesoangioblasts under physiological growth conditions

et al. 2008

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Mouse A6 mesoangioblasts express Hsp70 even in the absence of cellular stress. Its expression and its intracellular localization were investigated under normal growth conditions and under hyperthermic stress. Immunofluorescence assays indicated that without any stress a fraction of Hsp70 co-localized with actin microfilaments, in the cell cortex and in the contractile ring of dividing cells, while the Hsc70 chaperone did not. Hsp70 immunoprecipitation assays confirmed that a portion of Hsp70 binds actin. Immunoblot assays showed that both proteins were present in the nucleus. After heat treatment Hsp70 and actin continued to co-localize in the leading edge of A6 cells but not on microfilaments. Although Hsp70 and Hsc70 are both basally synthesized they showed different cellular distribution, suggesting an Hsp70 different activity respect to the Hsc70 chaperone. Moreover, we found Hsp70 in the culture medium as it has been described in other cell types.

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Section: Discussionmentioning

confidence: 99%

“…Total protein extracts were prepared as previously described (Geraci et al 2006). For subcellular extracts trypsinized cells were harvested and washed in PBS.…”

Section: Preparation Of Total and Subcellular Extractsmentioning

confidence: 99%

Section: Hsp70 Localization In Heat-shocked A6 Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hsp70 localizes differently from chaperone Hsc70 in mouse mesoangioblasts under physiological growth conditions

et al. 2008

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“…A prominent effect of cadmium is the induction of oxidative stress both in vivo and in cultured cells (11)(12)(13)(14)(15). As a response to cadmium-induced stress, many cells activate the synthesis of heat shock proteins (Hsps) (10,16,17), in a heat shock factor (HSF)-dependent manner (17).…”

Section: Introductionmentioning

confidence: 99%

The effect of cadmium on brain cells in culture

Gerspacher

Scheuber²,

Schiera³

et al. 2009

Int J Mol Med

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Abstract. Cadmium is a long-living heavy metal, abundantly present in the environment, which accumulates in the body. In this study, we investigated the effects of cadmium on the expression of molecular chaperones, and of certain cell-specific proteins, in a variety of brain cell types in culture, namely primary cultures of rat cortical neurons and astrocytes, a brain capillary endothelial cell line (RB4E.B cells), and pheochromocytoma cells (PC12), induced or not to differentiate by NGF treatment. The metal induces a dose-dependent increase of Hsp70 in all cell types. Responses to the metal are cellspecific in the case of Hsc70 and Hsp90: i) in astrocytes, as well as in PC12 cells, cadmium has no significant effect; ii) in endothelial cells, an increase of both proteins is clearly observable from 20 μM cadmium; iii) both Hsp90 and Hsc70 decrease in neurons treated with high doses of cadmium. Damage to the cytoskeleton in treated cells was also evident. Finally, we report that the metal at high doses induces a decrease of PIPPin (also known as CSD-C2), a putative RNAbinding protein highly expressed in neurons and probably involved in the regulation of histone replacement variant expression.

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Membrane vesicles containing matrix metalloproteinase‐9 and fibroblast growth factor‐2 are released into the extracellular space from mouse mesoangioblast stem cells

Candela

Geraci

Turturici

et al. 2010

Journal Cellular Physiology

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Certain proteins, including fibroblast growth factor-2 (FGF-2) and matrix metalloproteinase-9 (MMP-9), have proved very effective in increasing the efficacy of mesoangioblast stem cell therapy in repairing damaged tissue. We provide the first evidence that mouse mesoangioblast stem cells release FGF-2 and MMP-9 in their active form through the production of membrane vesicles. These vesicles are produced and turned over continuously, but are stable for some time in the extracellular milieu. Mesoangioblasts shed membrane vesicles even under oxygen tensions that are lower than those typically used for cell culture and more like those of mouse tissues. These findings suggest that mesoangioblasts may themselves secrete paracrine signals and factors that make damaged tissues more amenable to cell therapy through the release of membrane vesicles.

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Stress response in mesoangioblast stem cells

Cited by 14 publications

References 31 publications

Hsp70 localizes differently from chaperone Hsc70 in mouse mesoangioblasts under physiological growth conditions

Hsp70 localizes differently from chaperone Hsc70 in mouse mesoangioblasts under physiological growth conditions

The effect of cadmium on brain cells in culture

Membrane vesicles containing matrix metalloproteinase‐9 and fibroblast growth factor‐2 are released into the extracellular space from mouse mesoangioblast stem cells

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