Concentration-dependent response of estrone-degrading bacterial community in activated sludge analyzed by microautoradiography-fluorescence in situ hybridization

Thayanukul, Parinda; Zang, Kaisai; Janhom, Tansiphorn; Kurisu, Futoshi; Kasuga, Ikuro; Furumai, Hiroaki

doi:10.1016/j.watres.2010.07.031

Cited by 37 publications

(28 citation statements)

References 43 publications

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“…Both synthetic and natural forms occur in wastewater and their removal in biological treatment systems is poorly understood and highly unpredictable (Sumpter, 2009). The relative abundance of taxa responsible for the degradation of even the best removed estrogens, e.g., E1, are indeed rare, representing less than 2% of the microbial community (Zang et al, 2008; Thayanukul et al, 2010). …”

Section: Introductionmentioning

confidence: 99%

Microbial community assembly, theory and rare functions

Pholchan¹,

Baptista²,

Davenport³

et al. 2013

Front. Microbiol.

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Views of community assembly have traditionally been based on the contrasting perspectives of the deterministic niche paradigm and stochastic neutral models. This study sought to determine if we could use empirical interventions conceived from a niche and neutral perspective to change the diversity and evenness of the microbial community within a reactor treating wastewater and to see if there was any associated change in the removal of endocrine disrupting compounds (EDCs). The systematic removal of EDCs and micropollutants from biological treatment systems is a major challenge for environmental engineers. We manipulated pairs of bioreactors in an experiment in which “niche” (temporal variation in resource concentration and resource complexity) and “neutral” (community size and immigration) attributes were changed and the effect on the detectable diversity and the removal of steroidal estrogens was evaluated. The effects of manipulations on diversity suggested that both niche and neutral processes are important in community assembly. We found that temporal variation in environmental conditions increased diversity but resource complexity did not. Larger communities had greater diversity but attempting to increase immigration by adding soil had the opposite effect. The effects of the manipulations on EDC removal efficiency were complex. Decreases in diversity, which were associated with a decrease in evenness, were associated with an increase in EDC removal. A simple generalized neutral model (calibrated with parameters typical of wastewater treatment plants) showed that decreases in diversity should lead to the increase in abundance of some ostensibly taxa rare. We conclude that neither niche and neutral perspectives nor the effect of diversity on putative rare functions can be properly understood by naïve qualitative observations. Instead, the relative importance of the key microbial mechanisms must be determined and, ideally, expressed mathematically.

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Section: Introductionmentioning

confidence: 99%

Microbial community assembly, theory and rare functions

Pholchan¹,

Baptista²,

Davenport³

et al. 2013

Front. Microbiol.

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“…Previously, we detected E1‐incorporating bacteria using the MAR‐FISH technique at concentrations as low as 540 ng l −1 (Thayanukul et al . ). Most of them were Alphaproteobacteria, however, it was impossible to completely identify them at such low concentrations.…”

Section: Resultsmentioning

confidence: 97%

“…Thayanukul et al . () further revealed that different bacteria incorporated E1 at different concentrations of E1. However, the specific identity of the bacteria is yet to be clarified.…”

Section: Introductionmentioning

confidence: 84%

Identification of estrone-degrading Betaproteobacteria in activated sludge by microautoradiography fluorescent in situ hybridization

Kurisu

Zang

Kasuga

et al. 2015

Lett Appl Microbiol

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Estrone (E1) is one of the natural estrogens, which can be degraded but is not always completely removed in wastewater treatment plants. E1 is suspected of causing the endocrine disruption of aquatic organisms in the receiving water body. We identified dominant E1-incorporating bacteria, which should include E1-degrading bacteria, in activated sludge treating domestic wastewater. Sphaerotilus-Leptothrix-related bacteria, which had never been reported in the previous attempts based on culture-dependent approach, occupied 60-80% of the E1-incorporating bacteria. This study demonstrates the identification of functionally active bacteria to degrade micro-pollutants at sub-milligram per litre level.

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“…However, by evaluating their models it was found that they have mostly used first-order and pseudo second-order kinetic models with respect to VSS in their models and did not incorporate X C which in this study has been found to be an important parameter to estimate the fate of MCs in AS systems. It should be noted that the concentration of X C was estimated mathematically in this study and further experimental studies are needed to better understand the role of specific microorganism in degradation of MCs in AS systems by using fluorescent in situ hybridization (FISH) with group-specific rRNA-targeted probes as described in the literature (Thayanukul et al, 2010;Zang et al, 2008).…”

Section: Parameter Estimation Resultsmentioning

confidence: 99%