Identification of estrone-degrading Betaproteobacteria in activated sludge by microautoradiography fluorescent <i>in situ</i>
 hybridization

Kurisu, Futoshi; Zang, Kaisai; Kasuga, Ikuro; Furumai, Hiroaki; Yagi, Osami

doi:10.1111/lam.12407

Cited by 10 publications

(4 citation statements)

References 36 publications

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“…frequently employed for in situ identification of microorganisms with specific substrate uptakes (Okabe et al, 2004;Hagman et al, 2008;McIlroy et al, 2010). Despite this, MAR-FISH has only been used as a microbial identification technique for a few micropollutants such as gemfibrozil (Lolas et al, 2012) and estrone (Kurisu et al, 2015;Zang et al, 2008).…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms

et al. 2018

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A tiered process was developed to assess the transformation, CO formation and uptake of four organic micropollutants by carrier-attached microorganisms from two municipal wastewater treatment plants. At the first tier, primary transformation of ibuprofen, naproxen, diclofenac, and mecoprop by carrier-attached microorganisms was shown by the dissipation of the target compounds and the formation of five transformation products using LC-tandem MS. At the second tier, the microbial cleavage of the four organic micropollutants was confirmed with C-labeled micropollutants through liquid scintillation counting of theCO formed. At the third tier, microautoradiography coupled with ﬂuorescence in situ hybridization (MAR-FISH) was used to screen carrier-attached microorganisms for uptake of the four radiolabeled micropollutants. Results from the MAR-FISH screening indicated that only a small fraction of the microbial community (≤1‰) was involved in the uptake of the radiolabeled micropollutants and that the responsible microorganisms differed between the compounds. At the fourth tier, the microbial community structure of the carrier-attached biofilms was analyzed by 16S rRNA gene amplicon sequencing. The sequencing results showed that the MAR-FISH screening targeted ∼80% of the microbial community and that several taxonomic families within the FISH-probed populations with MAR-positive signals (i.e. Firmicutes, Gammaproteobacteria, and Deltaproteobacteria) were present in both biofilms. From the broader perspective of organic micropollutant removal in biological wastewater treatment, the MAR-FISH results of this study indicate a high degree of microbial substrate specialization that could explain differences in transformation rates and patterns between micropollutants and microbial communities.

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Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms

et al. 2018

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“…The microautoradiography-fluorescence in situ hybridization (MAR-FISH) technique was applied to identify active oestrone-assimilating bacteria in activated sludge using [2,4,6,7-3 H(N)]oestrone as a tracer. Some studies have revealed that several active proteobacterial taxa incorporated trace oestrone (submicrogram per litter concentrations) in activated sludges, indicating that the main degraders of oestrogen in wastewater treatment plants are different from those reported in culture-dependent studies (Zang et al, 2008;Thayanukul et al, 2010;Kurisu et al, 2015). However, this technique has several disadvantages.…”

Section: Anaerobic Degradation Of Steroids Through the 23-seco Pathwaymentioning

confidence: 99%

Microbial degradation of steroid sex hormones: implications for environmental and ecological studies

Chiang

Wei

Wang

et al. 2019

Microbial Biotechnology

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Steroid hormones modulate development, reproduction and communication in eukaryotes. The widespread occurrence and persistence of steroid hormones have attracted public attention due to their endocrine-disrupting effects on both wildlife and human beings. Bacteria are responsible for mineralizing steroids from the biosphere. Aerobic degradation of steroid hormones relies on O 2 as a co-substrate of oxygenases to activate and to cleave the recalcitrant steroidal core ring. To date, two oxygen-dependent degradation pathwaysthe 9,10-seco pathway for androgens and the 4,5-seco pathways for oestrogens have been characterized. Under anaerobic conditions, denitrifying bacteria adopt the 2,3-seco pathway to degrade different steroid structures. Recent meta-omics revealed that microorganisms able to degrade steroids are highly diverse and ubiquitous in different ecosystems. This review also summarizes culture-independent approaches using the characteristic metabolites and catabolic genes to monitor steroid biodegradation in various ecosystems.

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“…FISH is a powerful tool in microbiology. For example, it has been used in the detection of community structures in biofilms (Almeida et al, 2011;Aoi, 2002), aquatic microbiome sampling (Dawson et al, 2012;Kurisu et al, 2015;Medlin and Orozco, 2017), cultural heritage conservation (La Cono and Urzi, 2003;Urzi and De Leo, 2001), in clinical samples of blood sera (Da Silva et al, 2015) and many other uses. FISH has been used for the localisation of bacteria, fungi and viruses within respective hosts (reviewed in (Kliot and Ghanim, 2016)).…”

Section: Introductionmentioning

confidence: 99%

FISHing in fungi: Visualisation of mushroom virus X in the mycelium of Agaricus bisporus by fluorescence in situ hybridisation

O’Connor

Coates

Eastwood

et al. 2020

Journal of Microbiological Methods

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Identification of estrone-degrading Betaproteobacteria in activated sludge by microautoradiography fluorescent in situ hybridization

Cited by 10 publications

References 36 publications

Transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms

Transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms

Microbial degradation of steroid sex hormones: implications for environmental and ecological studies

FISHing in fungi: Visualisation of mushroom virus X in the mycelium of Agaricus bisporus by fluorescence in situ hybridisation

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