Concentration effects of Winogradskyella sp. on the incidence and severity of amoebic gill disease

Embar-Gopinath, S; Crosbie, Philip; Nowak, Barbara F.

doi:10.3354/dao073043

Cited by 13 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…may exacerbate amoebic gill disease in Atlantic salmon though this is assumed to be a rather nonspecific relationship (Embar‐Gopinath et al . ). Tenacibaculum is a widely isolated gliding genus from marine fauna (http://www.bacterio.cict.fr/t/tenacibaculum.html) and the most well‐studied species, Tenacibaculum maritimum causes flexibacteriosis in a variety of fish species.…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Culturable microbiota of ranched southern bluefin tuna (Thunnus maccoyii Castelnau)

Valdenegro-Vega

Naeem

Carson³

et al. 2013

J Appl Microbiol

View full text Add to dashboard Cite

Aims: The Australian tuna industry is based on the ranching of wild southern bluefin tuna (SBT, Thunnus maccoyii). Within this industry, only opportunistic pathogens have been reported infecting external wounds of fish. This study aimed to identify different culturable bacteria present in three cohorts of SBT and to determine normal bacteria and potential pathogens in isolates from harvest fish and moribund/dead fish. Post-mortem changes in the microbiota were also studied. Methods and Results: Moribund/dead showed a greater proportion of members from the family Vibrionaceae than harvested fish; the latter presented mainly non-Vibrio species. In harvested fish spleens, Vibrio splendidus

show abstract

Section: Discussionmentioning

confidence: 97%

“…Little is known about the pathogenic potential of the genus Winogradskyella but Winogradskyella spp. may exacerbate amoebic gill disease in Atlantic salmon though this is assumed to be a rather nonspecific relationship (Embar-Gopinath et al 2006). Tenacibaculum is a widely isolated gliding genus from marine fauna (www.…”

Section: Discussionmentioning

confidence: 99%

Culturable microbiota of ranched southern bluefin tuna (Thunnus maccoyii Castelnau)

Valdenegro-Vega

Naeem

Carson³

et al. 2013

J Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…Previous studies in Tasmania investigated the role of salmonid gill bacteria on AGD (Bowman and Nowak 2004;Embar-Gopinath et al 2005;Embar-Gopinath et al 2006;Embar-Gopinath et al 2008). A culture-independent 16S rRNA gene-based approach was used to identify gill bacteria associated with AGD in Atlantic salmon infected in the laboratory or obtained from commercial sea cages (Bowman and Nowak 2004).…”

mentioning

confidence: 99%

“…infection relative to those exposed to a Staphylococcus sp. strain (Embar-Gopinath et al 2005;Embar-Gopinath et al 2006). In this study, the effect on P. perurans population growth, in vitro, at two different temperatures relevant to Scottish salmon aquaculture, 10°C and 15°C, was assessed using two different amoeba clonal cultures (i.e., B8, CE6) and a polyclonal (G) culture (Collins et al 2017) maintained at the MSS Marine Laboratory.…”

mentioning

confidence: 99%

Effects of temperature onParamoeba peruransgrowth in culture and the associated microbial community

2018

View full text Add to dashboard Cite

Population growth, in vitro, of three Paramoeba perurans cultures, one polyclonal (G) and two clonal (B8, CE6, derived from G), previously shown to differ in virulence (B8 > G > CE6), was compared at 10 and 15 °C. B8 showed a significantly higher increase in attached and in suspended amoebae over time at 15 and 10 °C, respectively. CE6 and G also had significantly higher numbers of suspended amoebae at 10 °C compared with 15 °C at experiment termination. However, in contrast to B8, numbers of attached amoebae were significantly higher at 10 °C in CE6 but showed a similar trend in G at the end of the experiment. Numbers of both suspended and attached amoebae were lower in B8 compared with CE6 and G. Significant differences in bacterial community composition and/or relative abundances were found, between cultures, between temperatures and between the same culture with and without amoebae, based on 16S rRNA Illumina MiSeq sequencing. Bacterial diversity was lower in B8 and CE6 compared with G, possibly reflecting selection during clonal isolation. The results indicate that polyclonal P. perurans populations may contain amoebae displaying different growth dynamics. Further studies are required to determine if these differences are linked to differences seen in the bacterial communities.

show abstract

“…Further investigation is warranted to investigate the relationship between lesion size and the impact of a freshwater bath. There has been minimal work done examining lesion size; Adams & Nowak (2001) examined lesion size to describe the distribution and structure of AGD lesions and Embar‐Gopinath, Butler & Nowak (2005) and Embar‐Gopinath, Crosbie & Nowak (2006) examined lesion size with respect to the presence of different salmonid gill bacteria. Lesion size in this study was similar to that in Embar‐Gopinath et al.…”

Section: Discussionmentioning

confidence: 99%

Further development of bithionol therapy as a treatment for amoebic gill disease in Atlantic salmon, Salmo salar L.

Florent¹,

Becker²,

Powell³

2009

Journal of Fish Diseases

View full text Add to dashboard Cite

This study examined the efficacy of bithionol as a prophylactic or therapeutic oral treatment for Atlantic salmon (AS), Salmo salar, affected by amoebic gill disease (AGD). Furthermore, it explored the interaction of bithionol oral therapy with the current standard treatment (a freshwater bath for at least 3 h). The efficacy of three medicated feeds was determined in the trial by feeding AGD-affected AS at 1% body weight (BW) day(-1) either oil coated commercial feed (control) or prophylactic and therapeutic bithionol at 25 mg kg(-1) feed. Feeding commenced 2 weeks prior to exposure to Neoparamoeba spp. at 300 cells L(-1) and continued for 49 days post-exposure (PE). Bithionol when fed as a 2-week prophylactic or therapeutic treatment at 25 mg kg(-1) feed delayed the onset of AGD pathology and reduced the percentage of gill filaments with lesions. Administration of a 3-h freshwater bath at 28 days PE significantly reduced amoeba numbers to a similar level across all treatments; in contrast, gross gill score and percent lesioned filaments were reduced to different extents, the control having a significantly higher score than both bithionol treatments. Following the freshwater bath, clinical signs of AGD increased at a similar level across all treatments, albeit controls were significantly higher than the bithionol treatments immediately following freshwater treatment. This study demonstrated that bithionol at 25 mg kg(-1) feed, when fed as a 2-week prophylactic or a therapeutic treatment, delayed and reduced the intensity of AGD pathology and warrants further investigation as a treatment for AGD-affected AS.

show abstract

Concentration effects of Winogradskyella sp. on the incidence and severity of amoebic gill disease

Abstract: To study the concentration effects of the bacterium Winogradskyella sp. on amoebic gill disease (AGD), Atlantic salmon Salmo salar were pre-exposed to 2 different doses (10 8 or 10 10 cells l

Cited by 13 publications

References 15 publications

Culturable microbiota of ranched southern bluefin tuna (Thunnus maccoyii Castelnau)

Culturable microbiota of ranched southern bluefin tuna (Thunnus maccoyii Castelnau)

Effects of temperature onParamoeba peruransgrowth in culture and the associated microbial community

Further development of bithionol therapy as a treatment for amoebic gill disease in Atlantic salmon, Salmo salar L.

Contact Info

Product

Resources

About