Concentration of alpha-lactalbumin from cow milk whey through expanded bed adsorption using a hydrophobic resin

Abstract

-The design of novel applications for the use of new high-density adsorbents in the direct recovery of proteins requires analysis of the fluid dynamics and mass transfer characteristics of the column used with the solid-liquid system. In this article we describe the operating parameters related to the use of a highdensity, hydrophobic resin (Streamline ® Phenyl) for the recovery of α-lactalbumin from cow milk whey in an expanded-bed adsorption mode of operation. The adsorption isotherm, kinetic curves and chromatographic curves for adsorption and elution were determined for α-lactalbumin recovered from whole whey. The experiments were used to confirm the theoretical model that involves the combination of resistance to the mass transfer in the liquid phase and diffusivity in the adsorbent resin pores. The model solution was obtained through the orthogonal collocation method. A nonlinear multivariable optimization method was used to fit the model to experimental results. A purity of 79% was obtained with the adsorption/elution protocol prior to any polishing procedure.

Selective and single step adsorption of α-lactalbumin from whole cow's milk on hydroxyapatite microbeads

“…2, α-LA adsorption onto the HA microbeads showed maximum adsorption capacity at 10.0 mM (pH 7.0) phosphate buffer (lower phosphate ion concentration) which is appropriate for hydroxyapatite surface charges [33]. α-LA has got isoelectric point at 4.7-5.1 [2] and at pH 7.0, it could be charged negatively and the native structure of apo α-LA is stable [34]. The negative charges on the surface of a α-LA provide calcium binding site for adsorption process.…”

“…Whey proteins more specifically contains α-LA and β-lactoglobulin [2] and used as food ingredients because of their surface-activity and colloid-stabilizing properties [3]. Alpha-LA is an important bioactive food protein due to its composition of cysteine and tryptophan which are essential amino acids [4].…”

Trends in whey protein fractionation

“…Using hydrophobic chromatography, Chaplin (1986) separated bovine whey proteins and caseins on a fast protein liquid chromatography column, phenyl-superose; Hahn et al (2003) compared different hydrophobic interaction media regarding dynamic binding capacity, recovery and mass transfer properties; and Conrado et al (2005) described the operating parameters related to the use of Streamline phenyl for the recovery of ALA from cow milk whey in an expanded-bed adsorption mode of operation using a theoretical model. ALA at a purity of 79% was obtained using this method.…”

Simulation of the breakthrough curves for the adsorption of α-lactalbumin and β-lactoglobulin to SP Sepharose FF cation-exchanger

“…They lumped both components as one entity in the porediffusion adsorption model as well as in a subsequent lumped kinetic elution model. Conrado et al [10] described, by means of the pore-diffusion model, the operating parameters related to the use of a hydrophobic resin (Streamline Phenyl) for the recovery of ␣-lactalbumin from cow's milk whey using expanded-bed adsorption.…”

“…Being the simplest of models as the name implies, the simple kinetic model will be first examined and appropriate correction factors for the isotherm and kinetic parameters will be introduced when necessary. The model has been used by various workers to simulate protein breakthrough curves [10,[16][17][18]. However, the approach of introducing correction factors was not adopted.…”