Concentration of fungal ligninolytic enzymes by ultrafiltration and their use in distillery effluent decolorization

Pant, Deepak; Adholeya, Alok

doi:10.1007/s11274-009-0079-2

Cited by 61 publications

(20 citation statements)

References 32 publications

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“…Previously, Soares et al [38] had also reported that increasing concentration of laccase increased the rate of dye decolorization. Similarly, Pant and Adholeya [39] reported that the concentrated laccase from P. florida EM1303 obtained by ultrafiltration efficiently decolorized undiluted distillery effluent. This further proves the P. florida laccase utility in treating various effluents.…”

Section: Resultsmentioning

confidence: 86%

Production of laccase from Pleurotus florida using agro‐wastes and efficient decolorization of Reactive blue 198

Sathishkumar

Murugesan

Palvannan

2010

J. Basic Microbiol.

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Pleurotus florida NCIM 1243 produced laccase as the dominant lignolytic enzyme during the dye decolorization. Banana peel was the best substrate for extracellular laccase production under solid state fermentation when compared to mandarin peel and cantaloupe peel. The maximum activity of laccase (5.4 U/g) was detected on the 10 day. The ratio of banana peel: mandarin peel: cantaloupe peel (5:2:3) showed increased production of laccase (6.8 U/g). P. florida produced two extracellular laccase isoenzymes (L1 and L2). The half life of laccase at 60 degrees C was 2 h and at 4 h it retained 25% residual activity. P. florida laccase showed high thermostability and an interesting difference was noticed in the behavior of laccase isoenzymes at different temperature. The L1 isoenzyme of laccase showed remarked thermostability at 60 degrees C in the native PAGE when compared to L2 isoenzyme. The optimum pH, temperature and enzyme concentration for maximum decolorization was found to be 4.5, 60 degrees C and 1.2 U/ml, respectively. Partially purified laccase enzyme showed excellent decolorization activity to Reactive blue 198. The maximum decolorization (96%) was observed at lower dye concentrations (50-100 ppm) which decreased markedly when the dye concentration was increased beyond 150 ppm. The thermostable laccase of P. florida could be effectively used to decolorize the synthetic dyes in the textile effluent and other biotechnological applications.

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Section: Resultsmentioning

confidence: 86%

Production of laccase from Pleurotus florida using agro‐wastes and efficient decolorization of Reactive blue 198

Sathishkumar

Murugesan

Palvannan

2010

J. Basic Microbiol.

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“…Hence, in recent years, the importance of biological wastewater treatment systems has attracted the attention of workers the world over and has helped in developing efficient low cost waste treatment systems. Increased attention has been directed towards utilization of microbial activity for the mineralization and decolorization of vinasse (Pant and Adholeya 2007a, b, c;Pant and Adholeya 2009).…”

Section: Introductionmentioning

confidence: 99%

Selection of vinasse degrading microorganisms

Ferreira¹,

Aguiar²,

Pompeu³

et al. 2010

World J Microbiol Biotechnol

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Vinasse is a highly colored effluent with a high pollutant potential when disposed in the environment. Assays for decolorization of vinasse were performed, selecting the fungus Pleurotus sajor-caju CCB 020. The discoloration was cocominant with the increase of the activities of laccase, manganese-peroxidase and peroxidases. P. sajor-caju demonstrated a rise in biomass production (1.06 g 100 ml -1 ), and the enzyme activities such as laccase (varying from 400 to 450 IU l -1 ) reached between the 9th and 10th day of growth and for MnP at the 12th day of cultivation (varying from 60 to 100 IU l -1 ). It was concluded that the system P. sajor-caju/vinasse can be utilized as a bioprocess for color removal and degradation of complex vinasse compounds. It was observed an improvement in the characteristics and detoxification allowing its utilization as reused water, laccase and manganese-peroxidase enzymes production and for fungal biomass production with a high nutritional value.

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“…Especially TiO 2 , ZnO or their different modifications, such as metal-or alkaline-doped versions, have been used in photocatalytic processes as catalyst because of their huge capacity to produce higher amount of hydroxyl radicals [24][25][26]. On the other hand, some applications of biological wastewater treatment based on fungi have been used by researchers although satisfied results may not be reached [27].…”

Section: Introductionmentioning

confidence: 99%

Prediction of Photocatalytic Degradation and Mineralization Efficiencies of Basic Blue 3 Using $${{\rm TiO}_{2}}$$ TiO 2 by Nonlinear Modeling Based on Box–Behnken Design

Büyükada

2016

Arab J Sci Eng

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In the present study, nonlinear modeling of color and chemical oxygen demand (COD) removal of Basic Blue 3 as a fairly toxic textile dye by photocatalytic degradation using TiO 2 was investigated. A three replicates and one duplicate of Box-Behnken design with 45 runs, with three factors and three levels of catalyst concentration of 0.5-1.5 g/L, initial dye concentration of 20-100 mg/L, and initial pH of 3-11 were set to quantify the explanatory variables. ANOVA results showed that the best-fit multi-nonlinear regression models were cross-validated (R 2 pred ) accounting for 99.98-99.79 % and were expressed (R 2 adj ) accounting for 99.88-99.85 % of variation in decolorization (color removal) and mineralization (COD removal), respectively. In total, 0.51 g/L of TiO 2 , 100 mg/L of initial dye concentration, and initial pH of 7 were established as optimum operating parameters by leave-one-out optimization that resulted in 88.61 and 79.03 % for color and COD removal, respectively. 88.78 % of color removal and 76.95 % of COD removal were determined by cross-validation experiments at optimum conditions. Results showed that optimum point determination was successful.

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Concentration of fungal ligninolytic enzymes by ultrafiltration and their use in distillery effluent decolorization

Cited by 61 publications

References 32 publications

Production of laccase from Pleurotus florida using agro‐wastes and efficient decolorization of Reactive blue 198

Production of laccase from Pleurotus florida using agro‐wastes and efficient decolorization of Reactive blue 198

Selection of vinasse degrading microorganisms

Prediction of Photocatalytic Degradation and Mineralization Efficiencies of Basic Blue 3 Using $${{\rm TiO}_{2}}$$ TiO 2 by Nonlinear Modeling Based on Box–Behnken Design

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