2017
DOI: 10.3103/s1068367417050196
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Concentration of nitrite and nonthiol nitroso compounds in tissues as a high sensitive marker of leukocyte activity

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Cited by 3 publications
(4 citation statements)
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“…The challenge dose used in this experiment was in agreement with that employed in the ARVRIPS and in other studies [75,76,77]. The initiation of the inflammatory process in chickens after infection with a SE strain was confirmed based on the analysis of the content of nitrite and nitroso compounds in the blood [69,78]. An enzyme sensor [78] was used to detect these compounds in the chicken blood collected from the axillary vein.…”
Section: Methodssupporting
confidence: 60%
See 1 more Smart Citation
“…The challenge dose used in this experiment was in agreement with that employed in the ARVRIPS and in other studies [75,76,77]. The initiation of the inflammatory process in chickens after infection with a SE strain was confirmed based on the analysis of the content of nitrite and nitroso compounds in the blood [69,78]. An enzyme sensor [78] was used to detect these compounds in the chicken blood collected from the axillary vein.…”
Section: Methodssupporting
confidence: 60%
“…Subgroups I to IV were kept separately in four isolated boxes, with three cages in each box and 10 birds in each cage. Infection of the birds was performed intramuscularly according to the standard procedure [69], with the aim to induce an immune response as normally expected by an intramuscular vaccination and shown to be efficient in other studies [70,71,72,73,74]. The challenge dose used in this experiment was in agreement with that employed in the ARVRIPS and in other studies [75,76,77].…”
Section: Methodsmentioning
confidence: 95%
“…Presence of the inflammatory process in birds when infected with a pathogen was considered unambiguous if plasma concentrations of nitrite and N-nitroso compounds were more than 100 nM. An enzyme sensor was used to analyze nitrite and nitroso compounds in the blood of birds [ 69 ]. Additionally, to confirm the challenge of chickens with Salmonella , the pathological material of the intestines was plated on Modified Semisolid Rappaport-Vassiliadis Agar (Biokar Diagnostic, Allonne, France), cultured, and then analyzed using a rapid latex agglutination test (Salmonella Test Kit, Oxoid, Basingstoke, UK).…”
Section: Methodsmentioning
confidence: 99%
“…Challenge of the hens in S-II and S-IV was executed at 10 a.m. with the liquid culture of the SE strain in the amount of 8.69 lg colony-forming units (CFU) intramuscularly in the pectoral muscle using the standard technique [69] in order to trigger an immune response as ordinarily expected by an intramuscular vaccination. Prior to challenge, the bacterial strain was cultured overnight in the Rappaport medium at 35 • C. Nonchallenged chickens of S-I and S-III were injected with a similar volume of saline.…”
Section: Experimental Design and Samplingmentioning
confidence: 99%