Concentration of SARS-CoV-2-Infected Cell Culture Supernatants for Detection of Virus-like Particles by Scanning Electron Microscopy

Bideau, Marion Le; Robresco, Lea; Baudoin, Jean-Pierre; Scola, Bernard La

doi:10.3390/v14112388

Cited by 4 publications

(5 citation statements)

References 47 publications

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“…Kataoka et al examined the morphological and distribution changes of influenza A virus particles in host cells by using transmission electron microscopy (TEM) and scanning electron microscopy (SEM) methods . As a powerful tool, electron microscopy was also applied to detect and describe the emerging virus. , Electron microscope-based detection has the advantages of simplicity, directivity, and high resolution. However, the method requires expensive instruments and professional operators and is restricted by low sensitivity and susceptibility to matrix interference.…”

Section: Methods Of Foodborne Viruses Detectionmentioning

confidence: 99%

“…17 As a powerful tool, electron microscopy was also applied to detect and describe the emerging virus. 18,19 Electron microscope-based detection has the advantages of simplicity, directivity, and high resolution. However, the method requires expensive instruments and professional operators and is restricted by low sensitivity and susceptibility to matrix interference.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection Methods for Foodborne Viruses: Current State-of-Art and Future Perspectives

Yin

Zhang

Xiao

et al. 2023

J. Agric. Food Chem.

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Foodborne viruses have been recognized as important threats to food safety and human health. Rapid and accurate detection is one of the most crucial measures for food safety control. With the development of biology, chemistry, nanoscience, and related interdisciplines, detection strategies have been devised and advanced continuously. This review mainly focuses on the progress of detection methods for foodborne viruses. The current detection methods for foodborne viruses are summarized, including traditional electron microscopy and cultural isolation, immunoassay, molecular technology, biosensors, and newly emerging CRISPR/Cas-based detection technology. Furthermore, a comparison of the detection methods was objectively discussed. This review provides a comprehensive account of foodborne virus detection methods from fundamentals to state-of-the-art and illustrates the advantages and disadvantages of the current methods and proposes the future trends and directions for foodborne virus detection. It is hoped that this review can update current knowledge and present blueprints in order to accelerate futuristic development.

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Section: Methods Of Foodborne Viruses Detectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection Methods for Foodborne Viruses: Current State-of-Art and Future Perspectives

Yin

Zhang

Xiao

et al. 2023

J. Agric. Food Chem.

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“…Viruses in water can be concentrated based on their surface charge, using flocculation-precipitation methods or negative/positive membrane filters [ 51 , 52 ]. Size-based techniques can be used for viral concentration in both water and cell culture supernatants [ 53 , 54 ]. Selection of cell-free DNA (cfDNA) in plasma based on size can enrich viral DNA, as cfDNA size varies according to the species [ 55 ].…”

Section: What Is Metagenomics?mentioning

confidence: 99%

Bioinformatic Tools for NGS-Based Metagenomics to Improve the Clinical Diagnosis of Emerging, Re-Emerging and New Viruses

Ibañez-Lligoña

Colomer-Castell

González-Sánchez

et al. 2023

Viruses

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Epidemics and pandemics have occurred since the beginning of time, resulting in millions of deaths. Many such disease outbreaks are caused by viruses. Some viruses, particularly RNA viruses, are characterized by their high genetic variability, and this can affect certain phenotypic features: tropism, antigenicity, and susceptibility to antiviral drugs, vaccines, and the host immune response. The best strategy to face the emergence of new infectious genomes is prompt identification. However, currently available diagnostic tests are often limited for detecting new agents. High-throughput next-generation sequencing technologies based on metagenomics may be the solution to detect new infectious genomes and properly diagnose certain diseases. Metagenomic techniques enable the identification and characterization of disease-causing agents, but they require a large amount of genetic material and involve complex bioinformatic analyses. A wide variety of analytical tools can be used in the quality control and pre-processing of metagenomic data, filtering of untargeted sequences, assembly and quality control of reads, and taxonomic profiling of sequences to identify new viruses and ones that have been sequenced and uploaded to dedicated databases. Although there have been huge advances in the field of metagenomics, there is still a lack of consensus about which of the various approaches should be used for specific data analysis tasks. In this review, we provide some background on the study of viral infections, describe the contribution of metagenomics to this field, and place special emphasis on the bioinformatic tools (with their capabilities and limitations) available for use in metagenomic analyses of viral pathogens.

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“…The protocol for visualization of the iVPs was adapted from Le Bideau et al (2022). Briefly, samples were centrifuged over glass coverslips at 5.000 rcf for 45 min.…”

Section: Isolation and Testing Of The Sars-cov-2 Particlesmentioning

confidence: 99%

SARS-CoV-2 particles promote airway epithelial differentiation and ciliation

Gonzalez-Rubio,

Le-Trilling,

Baumann

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: The Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), which caused the coronavirus disease 2019 (COVID-19) pandemic, enters the human body via the epithelial cells of the airway tract. To trap and eject pathogens, the airway epithelium is composed of ciliated and secretory cells that produce mucus which is expelled through a process called mucociliary clearance.Methods: This study examines the early stages of contact between SARS-CoV-2 particles and the respiratory epithelium, utilizing 3D airway tri-culture models exposed to ultraviolet light-irradiated virus particles. These cultures are composed of human endothelial cells and human tracheal mesenchymal cells in a fibrin hydrogel matrix covered by mucociliated human tracheal epithelial cells.Results: We found that SARS-CoV-2 particles trigger a significant increase in ciliation on the epithelial surface instructed through a differentiation of club cells and basal stem cells. The contact with SARS-CoV-2 particles also provoked a loss of cell-cell tight junctions and impaired the barrier integrity. Further immunofluorescence analyses revealed an increase in FOXJ1 expression and PAK1/2 phosphorylation associated with particle-induced ciliation.Discussion: An understanding of epithelial responses to virus particles may be instrumental to prevent or treat respiratory infectious diseases such as COVID-19.

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Concentration of SARS-CoV-2-Infected Cell Culture Supernatants for Detection of Virus-like Particles by Scanning Electron Microscopy

Cited by 4 publications

References 47 publications

Detection Methods for Foodborne Viruses: Current State-of-Art and Future Perspectives

Detection Methods for Foodborne Viruses: Current State-of-Art and Future Perspectives

Bioinformatic Tools for NGS-Based Metagenomics to Improve the Clinical Diagnosis of Emerging, Re-Emerging and New Viruses

SARS-CoV-2 particles promote airway epithelial differentiation and ciliation

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