Concentration of uric acid removed during dialysis. estimated by multi wavelength and processed ultra violet absorbance spectra

Jerotskaja, Jana; Uhlin, Fredrik; Lauri, Kai; Tanner, Risto; Luman, Merike; Fridolin, Ivo

doi:10.1109/iembs.2010.5627831

Cited by 3 publications

(1 citation statement)

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“…The result does not support the earlier conclusion [4] that the UV absorbance at 254 nm should enable one to follow the elimination of accumulated plasma components with particular emphasis on slowly diffusible organic compounds of known or assumed toxicity. This study and several other earlier studies [19,20,21] have indicated that a major part of the UV signal originates from the small toxic solute of UA, which enables online monitoring of UA [22]. The small water soluble molecule, UA, has the most important role at the wavelength 280 nm where it is responsible for a major part of the total UV absorbance: the mean contribution of UA was about half (48%).…”

Section: Discussionsupporting

confidence: 61%

Do Only Small Uremic Toxins, Chromophores, Contribute to the Online Dialysis Dose Monitoring by UV Absorbance?

Arund

Tanner

Uhlin

et al. 2012

Toxins

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The aim of this work was to evaluate the contributions of the main chromophores to the total UV absorbance of the spent dialysate and to assess removal dynamics of these solutes during optical on-line dialysis dose monitoring. High performance chromatography was used to separate and quantify UV-absorbing solutes in the spent dialysate sampled at the start and at the end of dialysis sessions. Chromatograms were monitored at 210, 254 and 280 nm routinely and full absorption spectra were registered between 200 and 400 nm. Nearly 95% of UV absorbance originates from solutes with high removal ratio, such as uric acid. The contributions of different solute groups vary at different wavelengths and there are dynamical changes in contributions during the single dialysis session. However, large standard deviation of the average contribution values within a series of sessions indicates remarkable differences between individual treatments. A noteworthy contribution of Paracetamol and its metabolites to the total UV absorbance was determined at all three wavelengths. Contribution of slowly dialyzed uremic solutes, such as indoxyl sulfate, was negligible.

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