Concentrations of angiogenic factors in follicular fluid and oocyte-cumulus complex culture medium from women undergoing in vitro fertilization: association with oocyte maturity and fertilization

Malamitsi‐Puchner, Ariadne; Sarandakou, Angeliki; Baka, Stavroula; Tziotis, John; Rizos, Dimitrios; Hassiakos, Dimitrios; Creatsas, George

doi:10.1016/s0015-0282(01)01854-4

Cited by 56 publications

(37 citation statements)

References 22 publications

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“…A significantly higher FF concentration of VEGF has been reported in oocytes failing to fertilize, and high FF VEGF levels also have been associated with poor embryo quality and low pregnancy rates in IVF (25)(26)(27). In the present study, the concentration of VEGF in small antral follicles was very low, as only 6 out of 15 follicles contained measurable VEGF.…”

Section: Discussioncontrasting

confidence: 37%

Levels of the epidermal growth factor-like peptide amphiregulin in follicular fluid reflect the mode of triggering ovulation: a comparison between gonadotrophin-releasing hormone agonist and urinary human chorionic gonadotrophin

Humaıdan

Westergaard

Mikkelsen

et al. 2011

Fertility and Sterility

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Section: Discussioncontrasting

confidence: 37%

Levels of the epidermal growth factor-like peptide amphiregulin in follicular fluid reflect the mode of triggering ovulation: a comparison between gonadotrophin-releasing hormone agonist and urinary human chorionic gonadotrophin

Humaıdan

Westergaard

Mikkelsen

et al. 2011

Fertility and Sterility

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“…In another study, increased intrafollicular ADM is suggested to be a marker of decreased ovarian response in IVF [12]. A positive association between elevated follicular fluid angiogenin concentration and oocyte maturity is also established [11].…”

Section: Discussionmentioning

confidence: 89%

“…It is well established that an adequate vascular supply is essential for ovarian follicle development [10]. The angiogenic factors, including adrenomedullin (ADM), vascular endothelial growth factor (VEGF), and angiogenin, present in follicular fluid are reported to play critical roles in the process of folliculogenesis and oocyte maturation due to their vasodilator, vasorelaxant, and angiogenic effects [11,12].…”

Section: Introductionmentioning

confidence: 99%

Intrafollicular interleukin-8, interleukin-12, and adrenomedullin are the promising prognostic markers of oocyte and embryo quality in women with endometriosis

Singh

Dutta

Chattopadhyay

et al. 2016

J Assist Reprod Genet

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Purpose The study aimed to investigate key intrafollicular prognostic factors among various cytokines and angiogenic molecules for prediction of mature oocytes and good-quality embryos in women with endometriosis undergoing in vitro fertilization (IVF). Methods Paired follicular fluid and serum samples were collected from 200 women with advanced stage endometriosis and 140 normal ovulating women during oocyte retrieval. The concentrations of cytokines (pro-inflammatory: IL-1β, TNF-α, IL-2, IL-8, IL-12, IFN-γ; anti-inflammatory: IL-4, IL-6, IL-10) and angiogenic molecules (vascular endothelial growth factor (VEGF), adrenomedullin, angiogenin) were determined in follicular fluid and serum using ELISA. Expression of these molecules was subjected to multivariate analysis for the identification of major predictive markers of oocyte and embryo quality. Receiver operating characteristic (ROC) curve was applied to determine the best cutoff point for the discrimination between mature and immature oocytes in these women. Results Significant increases in levels of cytokines and angiogenic molecules were observed in women with endometriosis compared to controls (P < 0.001). From the validated partial least squares-discriminant analysis (PLS-DA) model, IL-8, IL-12, and adrenomedullin were identified as the most important factors contributing to endometriosis and were negatively associated with oocyte maturity and embryo quality. Conclusion The levels of IL-8, IL-12, and adrenomedullin may be good indicators of embryo and oocyte quality in endometriosis patients undergoing IVF. Further studies are necessary to ascertain the potential of these markers for oocyte and embryo developmental competence which may help improve the chances of a successful IVF in endometriosis patients.

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“…Previous studies have suggested that an imbalance in endometrial angiogenesis and a too rapidly formed vascular network may result in failed implantation and first-trimester miscarriage [Banerjee et al 2013;Lash et al 2012;Plaisier et al 2009;Su et al 2011]. Furthermore, analyses of fertility-related soluble mediators in human uterine fluid have identified the angiogenic factor VEGF as a key regulator of embryo implantation [Hannan et al 2011], with high levels of VEGF in follicular fluid being associated with low fertilization rates [Malamitsi-Puchner et al 2001], and poor quality embryos [Barroso et al 1999]. HRG has been reported to interact with molecules such as TSP, VEGF, and various members of the fibroblast growth factor family [Jones et al 2005;Wake et al 2009].…”

Section: Discussionmentioning

confidence: 99%

Histidine-rich glycoprotein derived peptides affect endometrial angiogenesisin vitrobut has no effect on embryo development

Lindgren

Hreinsson

Helmestam

et al. 2016

Systems Biology in Reproductive Medicine

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(2016) Histidine-rich glycoprotein derived peptides affect endometrial angiogenesis invitro but has no effect on embryo development, Systems Biology in Reproductive Medicine, 62:3, 192-200, DOI: 10.3109/19396368.2016.1156785 To link to this article: https://doi.org/10. 3109/19396368.2016 Histidine-rich glycoprotein (HRG) is an abundant plasma protein involved in multiple biological processes including immunology, vascularisation, and coagulation. These processes are of importance in regulating embryo development and implantation. A specific polymorphism in the HRG gene, HRG C633T, has an impact on various aspects of fertility, such as oocyte quality, endometrial receptivity, and possibly the capacity of the embryo itself to implant. To further examine the potential role of the HRG C633T polymorphism in regulating endometrial angiogenesis and on embryo development, two HRG peptides were constructed. These HRG peptides correspond to the amino acids 169-203 of the protein which, in turn, reflects the C633T polymorphism in the gene. The HRG proline or serine peptides were added to cultures of primary human endometrial endothelial (HEE) cells and to human embryos in vitro. The HRG peptides inhibited vascular endothelial growth factor (VEGF) induced proliferation and migration and promoted tube formation of HEE cells. The embryos were monitored using a time-lapse system (EmbryoScope®). Except for a prolonged time from first cleavage after thawing to development of the morula, no difference in embryo morphokinetics or embryo quality was noted in human embryos cultured in the presence of the HRG proline peptide. Taken together, these results suggest that treatment with a specific HRG peptide might prime the endometrium for implantation and be beneficial for adequate placentation. However, addition of a specific HRG proline peptide to human embryos has no beneficial effects in terms of embryo development.

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Concentrations of angiogenic factors in follicular fluid and oocyte-cumulus complex culture medium from women undergoing in vitro fertilization: association with oocyte maturity and fertilization

Cited by 56 publications

References 22 publications

Levels of the epidermal growth factor-like peptide amphiregulin in follicular fluid reflect the mode of triggering ovulation: a comparison between gonadotrophin-releasing hormone agonist and urinary human chorionic gonadotrophin

Levels of the epidermal growth factor-like peptide amphiregulin in follicular fluid reflect the mode of triggering ovulation: a comparison between gonadotrophin-releasing hormone agonist and urinary human chorionic gonadotrophin

Intrafollicular interleukin-8, interleukin-12, and adrenomedullin are the promising prognostic markers of oocyte and embryo quality in women with endometriosis

Histidine-rich glycoprotein derived peptides affect endometrial angiogenesisin vitrobut has no effect on embryo development

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