Concentrations of melatonin, thyroxine, 17β-estradiol and 11-ketotestosterone in round goby (Neogobius melanostomus) in different phases of the reproductive cycle

Guellard, Tatiana; Kalamarz-Kubiak, Hanna; Kulczykowska, Ewa

doi:10.1016/j.anireprosci.2019.02.014

Cited by 7 publications

(9 citation statements)

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“…It was investigated that E 2 and T 4 are integral in affecting the gonadal tissues in both females and males of N. melanostomus during the timing of initiation of spawning and also the active spawning phase (Guellard et al 2019 ). The present study indicates that exposure to E 2 enhances T 4 plasma concentrations in both sexes of N. melanostomus .…”

Section: Discussionmentioning

confidence: 99%

“…In regressing ovaries of N. melanostomus in the non-spawning phase, exogenous E 2 also induced PG oocytes’ growth. Furthermore, it was observed that in N. melanostomus as in other fish females, high concentration of E 2 in plasma and gonads may also be caused by persisting steroidogenesis of atretic vitellogenic oocytes (Rinchard et al 1993 ; Guellard et al 2019 ). It is possible that exogenous estradiol strengthens not only the steroidogenesis in atretic oocytes but also in previtellogenic oocytes, accelerating their development in N. melanostomus .…”

Section: Discussionmentioning

confidence: 99%

“…Plasma Mel was assayed using a total melatonin radioimmunoassay (RIA) kit (RE29301, IBL International, Hamburg, Germany), with preceding extraction procedure according to the method presented by Kulczykowska et al ( 2007 ) and modified by Guellard et al ( 2019 ). The solid-phase extraction was carried out on Octadecyl C18 Speedisk Column, 10 μm (J.T.…”

Section: Methodsmentioning

confidence: 99%

“…Plasma E 2 was measured using RIA kit (ESTR-CTRIA, Cisbio Bioassays, Codolet, France) without preceding extraction procedure according to the manufacturer’s protocol validated in our laboratory (Kalamarz-Kubiak et al 2017 ; Guellard et al 2019 ). E 2 iodinated with 125 I was used as a tracer.…”

Section: Methodsmentioning

confidence: 99%

“…Plasma 11-KT was determined using a competitive enzyme immunoassay (EIA) kit (582751, Cayman Chemical, Ann Arbor, USA) with preceeding extraction procedure according to the method described by Sokołowska et al ( 2013 ) and modified by Guellard et al ( 2019 ). The plate was read at 412 nm using the absorbance microplate reader (Sunrise™, Tecan, Männedorf, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

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Effect of short-term intermittent exposure to waterborne estradiol on the reproductive physiology of the round goby (Neogobius melanostomus)

Guellard

Kalamarz-Kubiak

Arciszewski

2020

Environ Sci Pollut Res

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The objective of this study was to determine how the short-term exposure to a supraphysiological concentration of waterborne 17β-estradiol (E 2 ) influences on melatonin (Mel) and thyroxine (T 4 ) concentrations in plasma and E 2 and 11-ketotestosterone (11-KT) concentrations in plasma and gonads in both sexes of round goby ( Neogobius melanostomus ) during the pre-spawning, spawning, late spawning and non-spawning phases. The experimental protocol was based on short-term, repeated exposures of fish to a supraphysiological dose of waterborne E 2 . Mel level was unchanged on exposure to E 2 during the investigated phases, and its role in determining a time frame for spawning in both sexes of round goby seems to be stable in those conditions. T 4 and sex steroids (E 2 and 11-KT) were sensitive to the exposure of E 2 , and those changes influence gonads by accelerating oocyte development, ovulation and regression and inhibiting spermatogenesis in this species. The results demonstrate that the physiological responses of fish in all investigated phases were altered over a short window of exposure, indicating that short-term exposure to a supraphysiological dose of E 2 may impact fish in the wild. Furthermore, round goby can be recommended as a very suitable model for studying endocrine disruptors, which is sensitive to even short exposure to E 2 . Electronic supplementary material The online version of this article (10.1007/s11356-020-09702-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%