Concentric zones for pheromone components in the mushroom body calyx of the moth brain

Namiki, Shigehiro; Takaguchi, Mitsuko; Seki, Yôtâro; Kazawa, Tomoki; Fukushima, Ryota; Iwatsuki, Chika; Kanzaki, Ryohei

doi:10.1002/cne.23219

Cited by 21 publications

(28 citation statements)

References 90 publications

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“…These findings are in agreement with previous reports in M. sexta (Homberg et al, 1988). Whether the neurons carrying information about the primary pheromone component form a different projection pattern in the calyces from those tuned to the secondary constituent, like in B. mori (Namiki et al, 2013), or whether the interspecific information has a unique representation, is impossible to determine from the current data. The reason why the two neuron types carrying information about plant odors and pheromones did not reveal different projection patterns in the AMIRA models may be related to the relatively complex innervation pattern of the individual neurons in the calyces.…”

Section: Discussionmentioning

confidence: 91%

“…Furthermore, the male-specific MGC-projections are subdivided; those originating in the toroid, carrying information about the major pheromone component, bombykol, target a more medially located region than the neurons tuned to the second component, bombykal (Kanzaki et al, 2003; Seki et al, 2005). A somewhat different kind of organization was recently reported for the calyces by the major pheromone component being represented in a region different from those of plant-odors and the minor pheromone component which display a large degree of overlap (Namiki et al, 2013). In studies on the fruit fly, Drosophila melanogaster , combining single-cell labeling and image registration, partly corresponding findings have been reported by spatially segregated areas for fruit and pheromone signals in the higher olfactory centers (Jefferis et al, 2007).…”

Section: Introductionmentioning

confidence: 78%

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Representation of pheromones, interspecific signals, and plant odors in higher olfactory centers; mapping physiologically identified antennal-lobe projection neurons in the male heliothine moth

Zhao

Kvello

Løfaldli

et al. 2014

Front. Syst. Neurosci.

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The arrangement of anatomically separated systems for information about general and pheromone odorants is well documented at the initial levels of the olfactory pathway both in vertebrates and insects. In the primary olfactory center of the moth brain, for example, a few enlarged glomeruli situated dorsally, at the entrance of the antennal nerve, are devoted to information about female-produced substances whereas a set of more numerous ordinary glomeruli (OG) receives input about general odorants. Heliothine moths are particularly suitable for studying central chemosensory mechanisms not only because of their anatomically separated systems for plant odors and pheromones but also due to their use of female-produced substances in communication across the species. Thus, the male-specific system of heliothine moths includes two sub-arrangements, one ensuring attraction and mating behavior by carrying information about pheromones released by conspecifics, and the other inhibition of attraction via signal information emitted from heterospecifics. Based on previous tracing experiments, a general chemotopic organization of the male-specific glomeruli has been demonstrated in a number of heliothine species. As compared to the well explored organization of the moth antennal lobe (AL), demonstrating a non-overlapping representation of the biologically relevant stimuli, less is known about the neural arrangement residing at the following synaptic level, i.e., the mushroom body calyces and the lateral horn. In the study presented here, we have labeled physiologically characterized antennal-lobe projection neurons in males of the two heliothine species, Heliothis virescens and Helicoverpa assulta, for the purpose of mapping their target regions in the protocerebrum. In order to compare the representation of plant odors, pheromones, and interspecific signals in the higher brain regions of each species, we have created standard brain atlases and registered three-dimensional models of distinct uniglomerular projection neuron types into the relevant atlas.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 78%

Representation of pheromones, interspecific signals, and plant odors in higher olfactory centers; mapping physiologically identified antennal-lobe projection neurons in the male heliothine moth

Zhao

Kvello

Løfaldli

et al. 2014

Front. Syst. Neurosci.

View full text Add to dashboard Cite

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“…The analysis of neurite distribution was basically the same as that described in a previous study 70 . To analyse the distribution of neurites ( Fig.…”

Section: Methodsmentioning

confidence: 99%

“…We mainly used intracellular recording technique with glass-sharp microelectrodes for single-cell recording. The recording and staining procedures were conducted as previously described 69,70 . Each moth was fixed in a plastic chamber, and its head was immobilized using a notched plastic yoke slipped between the head and the thorax.…”

Section: Methodsmentioning

confidence: 99%

Information flow through neural circuits for pheromone orientation

et al. 2014

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Moths use a sophisticated olfactory navigation strategy for resource localization. Here we investigate the neuronal circuits involved in sensory processing to generate locomotor commands for pheromone-source orientation in the moth. We identify a candidate pathway for pheromone processing in the protocerebrum using a mass-staining technique. Our intracellular recordings of pheromone responsiveness detect four major circuits, including a newly identified unstructured neuropil, the superior medial protocerebrum, which supplies output to the lateral accessory lobe (LAL), the premotor centre for walking commands. Interneurons innervating the lower division of the LAL elicited longer responses than those innervating the upper division. Descending interneurons innervating the lower division of the LAL showed a state-dependent flip-flop response. In contrast, input from other visual areas in the protocerebrum mostly converge onto the upper division of the LAL. These results reveal the basic organization of the LAL: the upper division is identified as a protocerebral hub that receives inputs from various areas, while the lower division generates long-lasting activity for locomotor command.

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“…ALT, antennal lobe tract; MGC, macroglomerular complex; OG, ordinary glomeruli. (modified from Namiki et al, 2013). …”

Section: Lateral Protocerebrummentioning

confidence: 99%

Molecular and neural mechanisms of sex pheromone reception and processing in the silkmoth Bombyx mori

2014

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Male moths locate their mates using species-specific sex pheromones emitted by conspecific females. One striking feature of sex pheromone recognition in males is the high degree of specificity and sensitivity at all levels, from the primary sensory processes to behavior. The silkmoth Bombyx mori is an excellent model insect in which to decipher the underlying mechanisms of sex pheromone recognition due to its simple sex pheromone communication system, where a single pheromone component, bombykol, elicits the full sexual behavior of male moths. Various technical advancements that cover all levels of analysis from molecular to behavioral also allow the systematic analysis of pheromone recognition mechanisms. Sex pheromone signals are detected by pheromone receptors expressed in olfactory receptor neurons in the pheromone-sensitive sensilla trichodea on male antennae. The signals are transmitted to the first olfactory processing center, the antennal lobe (AL), and then are processed further in the higher centers (mushroom body and lateral protocerebrum) to elicit orientation behavior toward females. In recent years, significant progress has been made elucidating the molecular mechanisms underlying the detection of sex pheromones. In addition, extensive studies of the AL and higher centers have provided insights into the neural basis of pheromone processing in the silkmoth brain. This review describes these latest advances, and discusses what these advances have revealed about the mechanisms underlying the specific and sensitive recognition of sex pheromones in the silkmoth.

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Concentric zones for pheromone components in the mushroom body calyx of the moth brain

Cited by 21 publications

References 90 publications

Representation of pheromones, interspecific signals, and plant odors in higher olfactory centers; mapping physiologically identified antennal-lobe projection neurons in the male heliothine moth

Representation of pheromones, interspecific signals, and plant odors in higher olfactory centers; mapping physiologically identified antennal-lobe projection neurons in the male heliothine moth

Information flow through neural circuits for pheromone orientation

Molecular and neural mechanisms of sex pheromone reception and processing in the silkmoth Bombyx mori

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