Conception d'un micro-injecteur matriciel pour la synthèse in situ d'oligonucléotides sur les puces ADN

Phou, Ty; Jugieu, David; Gué, Anne Marie

doi:10.1051/lhb/2003096

Cited by 2 publications

(1 citation statement)

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“…5 However, to achieve this goal requires parallel, simultaneous and automated dispensing of a multitude of samples/reagents in a rapid fashion. 6 Controlled volume dispensing and transport in these devices is achieved in a variety of ways utilizing either active or passive schemes. The active schemes primarily utilize electrical forces at microscales to control individual droplet motion.…”

Section: Introductionmentioning

confidence: 99%

Liquid DEP actuation and precision dispensing of variable volume droplets

2010

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Droplet based microfluidic systems, in recent years, have demonstrated numerous advantages and exciting potential for Lab-On-a Chip applications. In order to fully realize the potential benefits of this technology, one requires precision dispensing and manipulation of droplets of known volume and sample concentration, in a rapid and controlled manner. In this article, we demonstrate the rapid and controlled microactuation of aqueous samples and subsequent dispensing of variable volume droplets in nanolitre to picolitre regime by using a coplanar tapered electrode structure that leverages the phenomena of liquid dielectrophoresis (L-DEP). The transient behavior of the tapered liquid jet departs significantly from that of a uniform liquid jet case and is not adequately explained in terms of a simplified lumped capacitance model as in the case of the uniform jet, during the L-DEP actuation. A more generalized numerical model is developed for the tapered actuation scheme to account for the experimental observations. We furthermore demonstrate that the density of the dispensed droplets can be proactively controlled by the judicious placement of electrode bumps and pinches in the electrode structure thus overcoming the limitations imposed by Rayleigh's instability criterion. The proposed droplet dispensing schemes are superior to existing L-DEP based dispensing schemes which are restricted in size and spacing of the dispensed droplets by Rayleigh's instability criteria and furthermore mostly restricted to equi-volume droplets.

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Section: Introductionmentioning

confidence: 99%

Liquid DEP actuation and precision dispensing of variable volume droplets

2010

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Control of serial microfluidic droplet size gradient by step-wise ramping of flow rates

Collins

Lee

2006

Microfluid Nanofluid

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This paper describes a method to control and detect droplet size gradient by step-wise flow rate ramping of water-in-oil droplets in a microfluidic device. The droplets are generated in a cross channel device with two oil inlets and a water inlet. The droplet images are captured and analyzed in a time sequence in order to quantify the droplet generation frequency. It is demonstrated that by controlling the ramping of the oil flow rates it is possible to manipulate the ramping of droplet sizes. Increasing or decreasing of droplet sizes is achieved for a step-wise triangular ramping profile of the oil flow rate. The dynamic behavior of droplets due to the step-wise flow pulses is investigated. Uniform linear size ramping of water-in-oil droplets from 73 to 83 lm in diameter is generated with an oil flow ramping range from 1 to 11 lL/min in a minimum of five steps while water flow rate is held constant at 2 lL/min.

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Conception d'un micro-injecteur matriciel pour la synthèse in situ d'oligonucléotides sur les puces ADN

Cited by 2 publications

References 0 publications

Liquid DEP actuation and precision dispensing of variable volume droplets

Liquid DEP actuation and precision dispensing of variable volume droplets

Control of serial microfluidic droplet size gradient by step-wise ramping of flow rates

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