The objective of this study was to see how administration of Red Grape Seed Extract (RGSE) affected memory and Acetylcholinesterase (AChE) activity in D-Galactose-induced Albino rats' Cerebellum, Cerebral Cortex, Hippocampus, and Pons Medulla. Four groups of six animals were formed. D-Galactose, RGSE, and D-Gal+ RGSE groups were studied. After 30 days of RGSE therapy, the animals were subjected to behavioural tests before being euthanized and their brain structures and blood were collected. The D-Galactose group demonstrated a decrease in step-down latency. D-Galactose-induced memory impairment was prevented by the RGSE group. In the open field test, there were no discernible differences between the groups. When compared to the control group, the Dgalactose group displayed significantly higher AChE activity in all regions of the brain. However, AChE activity decreased significantly in the RGSE groups in the Cerebellum, Cerebral cortex, Hippocampus, and Pons medulla, whereas no significant alterations were seen in the combined therapy groups in any brain tissue when compared to the control group. Finally, the current data revealed that RGSE therapy inhibits the increase in AChE activity and, as a result, memory impairment in Albino rats, suggesting that this molecule can alter cholinergic neurotransmission and, as a result, improve cognition.