Concerns over the origin of NIH-CQV, a novel virus discovered in Chinese patients with seronegative hepatitis

Naccache, Samia N.; Hackett, John; Delwart, Eric; Chiu, Charles Y.

doi:10.1073/pnas.1317064111

Cited by 36 publications

(28 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study claimed this virus was highly prevalent in human patients with seronegative hepatitis, and phylogenetic analysis indicated that the NIH‐CQV was located at the interface of Parvoviridae and Circoviridae . The origin of this new virus was later questioned by Naccache and colleagues as a likely reagent contaminant (Naccache et al., ; Smuts et al., ; Editorial correction, ; Naccache et al., ). Our blast analysis (Table ) identified also eight circovirus‐like singletons (≥71% identity when identified by blastn; ≥43% identity when identified by blastx).…”

Section: Resultsmentioning

confidence: 99%

False-Positive Results in Metagenomic Virus Discovery: A Strong Case for Follow-Up Diagnosis

Rosseel

Pardon

Clercq

et al. 2014

Transbound Emerg Dis

View full text Add to dashboard Cite

A viral metagenomic approach using virion enrichment, random amplification and next-generation sequencing was used to investigate an undiagnosed cluster of dairy cattle presenting with high persistent fever, unresponsive to anti-microbial and anti-inflammatory treatment, diarrhoea and redness of nose and teat. Serum and whole blood samples were taken in the predicted hyperviraemic state of an animal that a few days later presented with these clinical signs. Bioinformatics analysis of the resulting data from the DNA virus identification workflow (a total of 32 757 sequences with average read length 335 bases) initially demonstrated the presence of parvovirus-like sequences in the tested blood sample. Thorough follow-up using specific real-time RT-PCR assays targeting the detected sequence fragments confirmed the presence of these sequences in the original sample as well as in a sample of an additional animal, but a contamination with an identical genetic signature in negative extraction controls was demonstrated. Further investigation using an alternative extraction method identified a contamination of the originally used Qiagen extraction columns with parvovirus-like nucleic acids or virus particles. Although we did not find any relevant virus that could be associated with the disease, these observations clearly illustrate the importance of using a proper control strategy and follow-up diagnostic tests in any viral metagenomic study.

show abstract

Section: Resultsmentioning

confidence: 99%

False-Positive Results in Metagenomic Virus Discovery: A Strong Case for Follow-Up Diagnosis

Rosseel

Pardon

Clercq

et al. 2014

Transbound Emerg Dis

View full text Add to dashboard Cite

show abstract

“…Presence of low-level HPV18 DNA sequences in The Cancer Genome Anatomy (TCGA) sequence database have been traced back to sequencing contaminating HeLa cells [61]. Sequencing-based discovery of a 'new human hepatitis virus' [62] actually turned out to be caused by retained RNA fragments from a marine phytoplankton virus adhering to diatomaceous earth (made from beach sand) used to make RNA isolation spin columns [63]. Endogenous retroviruses also provide rich opportunities to deceive scientists [64]: different viruses discovered in cell lines and naturally suspected to cause rhabdosarcoma and prostate cancer each represented independent examples in which endogenous retroviruses jumped from the host to a cell line when the cancer cells were passed through experimental animals [65].…”

Section: (G) Human Tumour Viruses Versus Rumour Virusesmentioning

confidence: 99%

Human oncogenic viruses: nature and discovery

Chang

Moore

Weiss

2017

Phil. Trans. R. Soc. B

View full text Add to dashboard Cite

Seven kinds of virus collectively comprise an important cause of cancer, particularly in less developed countries and for people with damaged immune systems. Discovered over the past 54 years, most of these viruses are common infections of humankind for which malignancy is a rare consequence. Various cofactors affect the complex interaction between virus and host and the likelihood of cancer emerging. Although individual human tumour viruses exert their malignant effects in different ways, there are common features that illuminate mechanisms of oncogenesis more generally, whether or not there is a viral aetiology. This article is part of the themed issue ‘Human oncogenic viruses’.

show abstract

“…Other viral hits were also recorded but assigned to contamination from the use of silica columns derived from marine diatoms for nucleic acid extraction, as previously reported for a small circular DNA virus (NIH-CQV) (Naccache et al, 2013(Naccache et al, , 2014Smuts et al, 2014). Because pools 1-27 were extracted with QIAamp viral RNA mini kits (Qiagen) and pools 28-51 were extracted using the magnetic beads method of the MagMax viral RNA extraction kit, we were able to compare the viral hits derived using both methods.…”

Section: Contamination Due To the Use Of Silica-containing Columnsmentioning

confidence: 98%

The plasma virome of febrile adult Kenyans shows frequent parvovirus B19 infections and a novel arbovirus (Kadipiro virus)

Ngoi

Siqueira

et al. 2016

Journal of General Virology

View full text Add to dashboard Cite

Viral nucleic acids present in the plasma of 498 Kenyan adults with unexplained fever were characterized by metagenomics analysis of 51 sample pools. The highest to lowest fraction of plasma pools was positive for parvovirus B19 (75 %), pegivirus C (GBV-C) (67 %), alpha anellovirus (59 %), gamma anellovirus (55 %), beta anellovirus (41 %), dengue virus genotype 2 (DENV-2) (16 %), human immunodeficiency virus type 1 (6 %), human herpesvirus 6 (6 %), HBV (4 %), rotavirus (4 %), hepatitis B virus (4 %), rhinovirus C (2 %), Merkel cell polyomavirus (MCPyV; 2 %) and Kadipiro virus (2 %). Ranking by overall percentage of viral reads yielded similar results. Characterization of viral nucleic acids in the plasma of a febrile East African population showed a high frequency of parvovirus B19 and DENV infections and detected a reovirus (Kadipiro virus) previously reported only in Asian Culex mosquitoes, providing a baseline to compare with future virome studies to detect emerging viruses in this region.

show abstract

Concerns over the origin of NIH-CQV, a novel virus discovered in Chinese patients with seronegative hepatitis

Cited by 36 publications

References 4 publications

False-Positive Results in Metagenomic Virus Discovery: A Strong Case for Follow-Up Diagnosis

False-Positive Results in Metagenomic Virus Discovery: A Strong Case for Follow-Up Diagnosis

Human oncogenic viruses: nature and discovery

The plasma virome of febrile adult Kenyans shows frequent parvovirus B19 infections and a novel arbovirus (Kadipiro virus)

Contact Info

Product

Resources

About