Concerted action of target‐site mutations and high <scp>EPSPS</scp> activity in glyphosate‐resistant junglerice (<i>Echinochloa colona</i>) from California

Alarcón‐Reverte, Rocío; García, Álvaro; Watson, Susan B.; Abdallah, Ibrahim; Sabaté, Sebastián; Hernández, María José; Dayan, Franck E.; Fischer, Albert J.

doi:10.1002/ps.3878

Cited by 60 publications

(85 citation statements)

References 79 publications

(131 reference statements)

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“…According to Carvalho et al (2012), sourgrass may present mechanisms that contribute to glyphosate resistance, including reduced glyphosate absorption and translocation, metabolism into nontoxic substances, or target-site mutations in the EPSPS gene. Others grass weed species, such as Echinochloa colona (Alarcón-Reverte et al, 2015), E. indica (Yu et al, 2015) and Lolium perenne ssp. multiflorum (Salas, Scott, Dayan, & Burgos, 2015), exhibited an RF ranging from 1.4 to 31.1 based on GR 50 and from 4 to 15 based on LD 50 , except for E. indica, which showed an FR that was > 182 (Yu et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…The glyphosate responses of these species were due to the different resistance mechanisms of each. Glyphosate resistance in Echinochloa colona was due to mutations at the Pro-106 position of the EPSPS gene and an enhanced basal activity (Alarcón-Reverte et al, 2015). The most resistant E. indica genotype evolved a double mutation, known as TIPS, at the Thr-102-Ile and Pro-106-Ser positions (Yu et al, 2015), and L. perenne ssp.…”

Section: Discussionmentioning

confidence: 99%

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<b>Glyphosate efficacy on sourgrass biotypes with suspected resistance collected in GR-crop fields

et al. 2018

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ABSTRACT. In Brazil, infestations of crop areas with glyphosate-resistant (GR) sourgrass (Digitaria insularis (L.) Fedde) biotypes has risen significantly, increasing crop production costs. Glyphosate efficacy on three biotypes (GO, BA and MT) of sourgrass with suspected resistance was evaluated. A susceptible biotype (MG) was used as the control. The results confirmed that the MG and GO biotypes were susceptible to glyphosate (control > 90%). The MG biotype exhibited growth reduction and mortality by 50% (GR 50 and LD 50 , respectively) with mean glyphosate doses of 243.7 and 431.6 g ae ha -1. The resistance index of the biotypes with suspected resistance ranged from 2.8 to 6.1 in relation to GR 50 and between 1.4 to 26.7 in relation to LD 50 . The glyphosate susceptibility ranking of the sourgrass biotypes was MG < GO < MT < BA. The MT and BA biotypes demonstrated high glyphosate resistance levels, and the GO biotype had a high potential to develop resistance. Farmers should avoid the application of glyphosate overdoses to minimize the selection pressure on weeds. Eficácia do glyphosate em biótipos de capim-amargoso com indício de resistência coletadas em cultivos RR RESUMO. No Brasil, as infestações de áreas agrícolas com biótipos de capim-amargoso resistentes ao glyphosate, resultam em aumento significativo nos custos de produção das culturas. Avaliou-se a eficácia do glyphosate em três biótipos de capim-amargoso com indício de resistência (GO, BA e MT) a este herbicida. Um biótipo suscetível (MG) foi utilizado como controle. Constatou-se que os biótipos MG e GO foram suscetíveis ao herbicida (controle > 90%). O biótipo MG apresentou redução do crescimento e mortalidade em 50% (GR 50 e C 50 , respectivamente) nas doses de glyphosate de 243,7 e 431,6 g ha -1. Os fatores de resistência dos biótipos com indício de resistência oscilaram entre 2,8 a 6,1 em relação ao GR 50 ; e entre 1,4 e ≥ 26,7 em relação ao C 50 . A ordem de susceptibilidade dos biótipos de capim-amargoso foi MG < GO < MT < BA. Estes últimos apresentaram elevado nível de resistência ao glyphosate, e o biótipo GO têm potencial para desenvolvê-la. Portanto, os agricultores devem evitar a aplicação de superdose de glyphosate a fim de reduzir a pressão de seleção das plantas daninhas.Palavras-chave: controle químico, Digitaria insularis, dose-resposta, fator de resistência.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

<b>Glyphosate efficacy on sourgrass biotypes with suspected resistance collected in GR-crop fields

et al. 2018

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“…Other glyphosate-resistant grass weeds such as: Bromus diandrus, Echinochloa colona, Eleusine indica, Lolium perenne spp. multiflorum, L. rigidum, Poa annua , among others (González-Torralva et al, 2012; Alarcón-Reverte et al, 2015; Chen et al, 2015; Cross et al, 2015; Fernandez et al, 2015; Salas et al, 2015; Yu et al, 2015; Malone et al, 2016), exhibited RI values that ranged between 3 to 19, and between 4 to <182 based on in their ED 50 or LD 50 , respectively. Differences in the level of glyphosate resistance between these species were due to various resistance mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…Different glyphosate concentrations in the tissue are related to differences in glyphosate efficacy (Alarcón-Reverte et al, 2015). Glyphosate will reach active metabolic sites, such as root and shoot meristems to act (Gomes et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

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First Resistance Mechanisms Characterization in Glyphosate-Resistant Leptochloa virgata

et al. 2016

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Leptochloa virgata (L.) P. Beauv. is an annual weed common in citrus groves in the states of Puebla and Veracruz, Mexico limiting their production. Since 2010, several L. virgata populations were identified as being resistant to glyphosate, but studies of their resistance mechanisms developed by this species have been conducted. In this work, three glyphosate-resistant populations (R8, R14, and R15) collected in citrus orchards from Mexico, were used to study their resistance mechanisms comparing them to one susceptible population (S). Dose-response and shikimic acid accumulation assays confirmed the glyphosate resistance of the three resistant populations. Higher doses of up to 720 g ae ha-1 (field dose) were needed to control by 50% plants of resistant populations. The S population absorbed between 7 and 13% more 14C-glyphosate than resistant ones, and translocated up to 32.2% of 14C-glyphosate to the roots at 96 h after treatment (HAT). The R8, R14, and R15 populations translocated only 24.5, 26.5, and 21.9%, respectively. The enzyme activity of 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) was not different in the S, R8 and R14 populations. The R15 Population exhibited 165.9 times greater EPSPS activity. Additionally, this population showed a higher EPSPS basal activity and a substitution in the codon 106 from Proline to Serine in the EPSPS protein sequence. EPSPS gene expression in the R15 population was similar to that of S population. In conclusion, the three resistant L. virgata populations show reduced absorption and translocation of 14C-glyphosate. Moreover, a mutation and an enhanced EPSPS basal activity at target-site level confers higher resistance to glyphosate. These results describe for the first time the glyphosate resistance mechanisms developed by resistant L. virgata populations of citrus orchards from Mexico.

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Target-site EPSPS Pro-106 mutations: sufficient to endow glyphosate resistance in polyploidEchinochloa colona?

Han

Widderick³

et al. 2015

Pest. Manag. Sci.

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This study shows that one or two EPSPS Pro-106 mutations are insufficient to confer field-rate glyphosate resistance in polyploidy E. colona at mild temperatures. However, control of these mutant plants at the glyphosate field rate is poor at high temperatures, probably owing to reduced glyphosate efficacy. Therefore, glyphosate should be applied during relatively mild (warm) temperature periods in the summer growing season to improve E. colona control.

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Concerted action of target‐site mutations and high EPSPS activity in glyphosate‐resistant junglerice (Echinochloa colona) from California

Cited by 60 publications

References 79 publications

<b>Glyphosate efficacy on sourgrass biotypes with suspected resistance collected in GR-crop fields

<b>Glyphosate efficacy on sourgrass biotypes with suspected resistance collected in GR-crop fields

First Resistance Mechanisms Characterization in Glyphosate-Resistant Leptochloa virgata

Target-site EPSPS Pro-106 mutations: sufficient to endow glyphosate resistance in polyploidEchinochloa colona?

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