Concise Review: Evidence for CD34 as a Common Marker for Diverse Progenitors

Sidney, Laura E.; Branch, Matthew J.; Dunphy, Siobhán E.; Dua, Harminder S.; Hopkinson, Andrew

doi:10.1002/stem.1661

Cited by 741 publications

(592 citation statements)

References 100 publications

(149 reference statements)

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“…C‐kit is a receptor that binds SCF, resulting in the mobilisation of calcium via a src family kinase and PI3K 22. In addition to its functional importance, c‐Kit is also essential for the differentiation of ICCs 39, 40,as CD34 is a typical marker for a wide variety of blood progenitor cells 41, and more generally, a marker of undifferentiated cells in various tissues 42.…”

Section: Discussionmentioning

confidence: 99%

Telocytes play a key role in prostate tissue organisation during the gland morphogenesis

Sanches

Maldarine

Zani

et al. 2017

J Cellular Molecular Medi

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Telocytes are CD34‐positive interstitial cells, known to exert several functions, one of which is a role in tissue organisation, previously demonstrated by telocytes in the myocardium. The existence of telocytes in the prostate has recently been reported, however, there is a lack of information regarding the function of these cells in prostate tissue, and information regarding the possible role of these cells in prostatic development. This study used immunofluorescence techniques in prostate tissue and prostatic telocytes in culture to determine the relationship between telocytes and prostate morphogenesis. Furthermore, immunofluorescent labelling of telocytes was performed on prostate tissue at different stages of early postnatal development. Initially, CD34‐positive cells are found at the periphery of the developing alveoli, later in the same region, c‐kit‐positive cells and cells positive for both factors are verified and CD34‐positive cells were predominantly observed in the interalveolar stroma and the region surrounding the periductal smooth muscle. Fluorescence assays also demonstrated that telocytes secrete TGF‐β1 and are ER‐Beta (ERβ) positive. The results suggest that telocytes play a changing role during development, initially supporting the differentiation of periductal and perialveolar smooth muscle, and later, producing dense networks that separate alveoli groups and form a barrier between the interalveolar region and periurethral smooth muscle. We conclude that telocytes play a relevant role in prostate tissue organisation during postnatal development.

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Section: Discussionmentioning

confidence: 99%

Telocytes play a key role in prostate tissue organisation during the gland morphogenesis

Sanches

Maldarine

Zani

et al. 2017

J Cellular Molecular Medi

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“…Ubiquitous CD34 expression is related to hematopoietic cells, and it serves as a marker to enrich hematopoietic stem cells for bone marrow transplants 50. CD34 + non‐hematopoietic lineage cells also represent a distinct subset of cells with enhanced progenitor activity, such as muscle satellite cells, interstitial cells, epithelial progenitors and CSKs 51. The CD34 + expressing cells could thus represent the quiescent CSKs or stromal progenitors,52 whereas CD34 − cells are the activated keratocytes not yet fully reverted to the quiescent condition 53.…”

Section: Discussionmentioning

confidence: 99%

Postnatal periodontal ligament as a novel adult stem cell source for regenerative corneal cell therapy

Yam

Teo

Setiawan

et al. 2018

J Cellular Molecular Medi

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Corneal opacities are a leading cause of global blindness. They are conventionally treated by the transplantation of donor corneal tissue, which is, restricted by a worldwide donor material shortage and allograft rejection. Autologous adult stem cells with a potential to differentiate into corneal stromal keratocytes (CSKs) could offer a suitable choice of cells for regenerative cell therapy. Postnatal periodontal ligament (PDL) contains a population of adult stem cells, which has a similar embryological origin as CSK, that is cranial neural crest. We harvested PDL cells from young adult teeth extracted because of non‐functional or orthodontic reason and differentiated them towards CSK phenotype using a two‐step protocol with spheroid formation followed by growth factor and cytokine induction in a stromal environment (human amnion stroma and porcine corneal stroma). Our results showed that the PDL‐differentiated CSK‐like cells expressed CSK markers (CD34, ALDH3A1, keratocan, lumican, CHST6, B3GNT7 and Col8A2) and had minimal expression of genes related to fibrosis and other lineages (vasculogenesis, adipogenesis, myogenesis, epitheliogenesis, neurogenesis and hematogenesis). Introduction of PDL spheroids into the stroma of porcine corneas resulted in extensive migration of cells inside the host stroma after 14‐day organ culture. Their quiescent nature and uniform cell distribution resembled to that of mature CSKs inside the native stroma. Our results demonstrated the potential translation of PDL cells for regenerative corneal cell therapy for corneal opacities.

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“…The CD34 is a highly glycosylated molecule made of single-chain glycoprotein with a weight of 110 kDa which is known as a cell surface marker in blood precursor cells as well as endothelial cells (Sidney et al, 2014). CD34 is activated as a receptor or ligand in the cell membrane through phosphorylating by kinases and transfers messages and the cells interaction (Sidney et al, 2014).…”

Section: Asian J Cellmentioning

confidence: 99%

“…CD34 is activated as a receptor or ligand in the cell membrane through phosphorylating by kinases and transfers messages and the cells interaction (Sidney et al, 2014). Expression of CD34 in colon stromal tumor is increased compared to normal tissue, so that it was significantly more than benign tumors in colon malignant tumors (Sidney et al, 2014). The CD34 expression is significantly associated with adenomas containing severe dysplasia and large adenomas (more than 1 cm) (Qasim et al, 2012;Sidney et al, 2014).…”

Section: Asian J Cellmentioning

confidence: 99%

“…The CD34 expression is significantly associated with adenomas containing severe dysplasia and large adenomas (more than 1 cm) (Qasim et al, 2012;Sidney et al, 2014). In many other immunohistochemical studies, the expression of this protein is used as a reliable marker to evaluate cancer tumors including: colon (Moreira et al, 2011;Sidney et al, 2014), blood (Borowitz et al, 1990), liver (Borowitz et al, 1990), penile (Martins et al, 2002), lung (Van de Rijn et al, 1994) and trichilemmal (Chaichamnan et al, 2010) cancers. The studies have shown the correlation between CD34 with many clinicopathologic factors as well as the ability of this marker as a prognostic factor.…”

Section: Asian J Cellmentioning

confidence: 99%

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Evaluating the Expression of CD34 Marker in Colorectal Adenocarcinoma and its Relationship with Clinicopathologic Factors

Behbehani¹,

Ranjbari²,

Rahim³

et al. 2015

Asian J. of Cell Biology

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Colorectal cancer is known as one of the most common types of cancer which the prevalence is increasing. According to reports, malignancies of the large intestine have highly broken out in Iran. Several studies have shown the correlation between the CD34 cell surface markers and some cancers. In the present study, the relationship between the protein expression and clinico-pathologic characteristics was evaluated. In this study, 40 paraffin blocks belonged to patients with colorectal cancer referring to Imam Khomeini Hospital in Ahvaz during [2003][2004][2005][2006][2007][2008][2009][2010][2011][2012][2013] were studied by immunohistochemistry method. From 40 samples, 22 were males and 18 females. Their mean age was 56±17.9 years and ranging from 22-87 years. The mean tumor size was 5.1 cm ranging 11-1.5 cm. The most common site of involvement was in the right colon (45%) and the rarest site in the transverse colon (10%) and 75% was in poorly-differentiated state in terms of tumor grade. Generally, marker expression was positive in 13 cases and negative in 27 cases. The CD34 expression was significantly correlated with tumor size, tumor depth, degree of differentiation as well as lymphatic, vascular and neural invasions. A positive correlation was observed between the marker expression and pathological characteristics. Therefore, CD34 marker expression can be effective as a marker in the diagnosis of pathological characteristics of colorectal cancer. However, further studies with a large population are recommended.

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Concise Review: Evidence for CD34 as a Common Marker for Diverse Progenitors

Cited by 741 publications

References 100 publications

Telocytes play a key role in prostate tissue organisation during the gland morphogenesis

Telocytes play a key role in prostate tissue organisation during the gland morphogenesis

Postnatal periodontal ligament as a novel adult stem cell source for regenerative corneal cell therapy

Evaluating the Expression of CD34 Marker in Colorectal Adenocarcinoma and its Relationship with Clinicopathologic Factors

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