Concurrent granulomatous nephritis and colitis associated with invasive Escherichia coli in a boxer dog

Cuesta-Garcia, Nerea; Marchesi, Franco; Ramsey, Ian; Simpson, Kenneth W.

doi:10.1016/j.jcpa.2014.10.159

Cited by 2 publications

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“…However, this was excluded by subsequent genotyping [ 3 ] and a normal oxidative burst in neutrophils from two GC affected dogs (The Neutrophil Monitoring Lab, NCI at Frederick, MD). Recent reports of intracellular E. coli in Boxers with concurrent PAS+ GC and PAS+ granulomatous cystitis (malakoplakia) and nephritis [ 1 , 50 , 51 , 52 ] suggest the potential for systemic inability to kill E. coli in GC-affected Boxers. The relative rarity of E. coli -associated GC and the need for fresh MDMs greatly limited our ability to perform functional analyses in more dogs, and we are working to extend evaluation of interactions between MDMs and E. coli prospectively.…”

Section: Discussionmentioning

confidence: 99%

Molecular and Phenotypic Characterization of Escherichia coli Associated with Granulomatous Colitis of Boxer Dogs

et al. 2020

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Invasive Escherichia coli is causally associated with granulomatous colitis (GC) of Boxer dogs and French Bulldogs. The virulence determinants of GC E. coli are unclear. E. coli isolated from 16 GC (36 strains) and 17 healthy control (HC: 33 strains) dogs were diverse in phylogeny, genotype, and serotype and lacked diarrheagenic genes. Genes encoding type II (gsp), IV (traC), and VI (hcp) secretion systems, long polar fimbriae (lpfA154/141), and iron acquisition (fyuA, chuA) were frequent in GC and HC. E. coli from 14/15 GC and 10/11 HC invaded Caco-2 better than non-pathogenic E. coli strain DH5α, with invasion correlated with motility and presence of chuA and colV. E. coli from all GC and 10/11 HC survived better than DH5α in J774 macrophages, with adherent-invasive E. coli (AIEC) in 60% GC and 73% HC. AIEC replicated in monocyte derived macrophages from a GC Boxer with CD48/SLAM risk haplotype but not the HC. Fluroquinolone resistant E. coli were less motile and invasive than fluoroquinolone sensitive (p < 0.05), and only 1/8 resistant strains met criteria for AIEC. In conclusion GC E. coli are diverse, resemble extraintestinal pathogenic E. coli (ExPEC), including AIEC, and can replicate in GC-susceptible macrophages. They are likely resident pathosymbionts that can opportunistically persist within macrophages of a GC-susceptible dog.

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Section: Discussionmentioning

confidence: 99%

Molecular and Phenotypic Characterization of Escherichia coli Associated with Granulomatous Colitis of Boxer Dogs

et al. 2020

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“…Although granulomatous colitis in dogs can appear histologically similar to mycobacterial granulomatous diseases of the intestines in other species (eg, Jonne’s disease in cattle), attempts to induce similar lesions in dogs using mycobacterial organisms have been unsuccessful, and infection with E coli has been implicated in the disease process instead 5,9,13,19 . One case of concurrent granulomatous colitis and nephritis was reported in a Boxer dog where E coli organisms were isolated from both sites 20 . Various treatment protocols have been attempted in dogs, with the most successful management consisting of antimicrobial therapy to clear underlying bacterial infection, in combination with symptomatic and supportive care 4,16,21,22 .…”

Section: Figurementioning

confidence: 99%

Correlation of cytology to histology in a case of canine granulomatous colitis in a Boxer dog

Sims

Nagle

Tolbert

et al. 2021

Veterinary Clinical Pathol

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A 2‐year‐old castrated male mixed breed dog presented to the North Carolina State Veterinary Teaching Hospital for chronic diarrhea with hematochezia and weight loss. Cytology performed on a rectal scraping revealed macrophages containing magenta, light pink, and variably blue granular inclusions, and phagocytosed material concerning for infectious organisms. Histopathology was consistent with granulomatous colitis and identified intra‐histiocytic bacterial organisms, confirmed by fluorescent in situ hybridization (FISH)—tissue culture‐confirmed Escherichia coli. Based on these findings, a diagnosis of granulomatous colitis was made. The patient was successfully treated with oral enrofloxacin, and near‐complete remission of signs was achieved within 6 weeks. This report describes a case of granulomatous colitis in a mixed breed dog, and is the first published description of the cytologic features of this uncommon disease, offering a valuable cytologic‐histologic correlation. In this case, the cytology was helpful in identifying features consistent with granulomatous colitis and prioritizing the differential diagnoses and diagnostic plan.

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Concurrent granulomatous nephritis and colitis associated with invasive Escherichia coli in a boxer dog

Cited by 2 publications

References 0 publications

Molecular and Phenotypic Characterization of Escherichia coli Associated with Granulomatous Colitis of Boxer Dogs

Molecular and Phenotypic Characterization of Escherichia coli Associated with Granulomatous Colitis of Boxer Dogs

Correlation of cytology to histology in a case of canine granulomatous colitis in a Boxer dog

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