Molecular and Phenotypic Characterization of Escherichia coli Associated with Granulomatous Colitis of Boxer Dogs

Dogan, Belgin; Zhang, Shiying; Kalla, Sarah E; Dogan, Esra I; Guo, Cindy; Ang, Chelston; Simpson, Kenneth W.

doi:10.3390/antibiotics9090540

Cited by 17 publications

(15 citation statements)

References 59 publications

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“…Susceptibility to AIEC invasion was linked to polymorphism in SLAM family members/CD48 gene in the recent canine GWAS study ( Hayward et al, 2016 ). Because AIEC has been isolated from subsets of human Crohn’s disease (CD) patients, and granulomatous lesions in canine GC mimic some CD lesions, microbiological comparisons between dog and human AIEC may elucidate a subset of CD pathophysiology ( Dogan et al, 2020 ). Some canine studies have demonstrated beneficial effects of fecal microbiota transfer in IBD ( Makielski et al, 2019 ; Niina et al, 2019 ), but factors that lead to improvement still need to be identified ( Cammarota and Ianiro, 2019 ).…”

Section: Caninementioning

confidence: 99%

Inflammation-Associated Microbiota Composition Across Domestic Animals

Hashimoto-Hill

Alenghat

2021

Front. Genet.

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Domestic animals represent important resources for understanding shared mechanisms underlying complex natural diseases that arise due to both genetic and environmental factors. Intestinal inflammation, particularly inflammatory bowel disease (IBD), is a significant health challenge in humans and domestic animals. While the etiology of IBD is multifactorial, imbalance of symbiotic gut microbiota has been hypothesized to play a central role in disease pathophysiology. Advances in genomic sequencing and analytical pipelines have enabled researchers to decipher the composition of the intestinal microbiota during health and in the context of naturally occurring diseases. This review compiles microbiome genomic data across domestic species and highlights a common occurrence of gut microbiome dysbiosis during idiopathic intestinal inflammation in multiple species, including dogs, cats, horses, cows, and pigs. Current microbiome data obtained from animals with intestinal inflammation are mostly limited to taxonomical analyses in association with broad clinical phenotype. In general, a pathogen or pathosymbiont were not detected. Rather, functional potential of the altered microbiota has been suggested to be one of the key etiologic factors. Among the domestic species studied, canine analyses are currently the most advanced with incorporation of functional profiling of microbiota. Canine IBD parallels features of the disease in humans, thus canines represent a strong natural model for human IBD. While deeper analyses of metagenomic data, coupled with host molecular analyses are needed, comparative studies across domestic species can reveal shared microbial alterations and regulatory mechanisms that will improve our understanding of intestinal inflammation in both animals and humans.

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Section: Caninementioning

confidence: 99%

Inflammation-Associated Microbiota Composition Across Domestic Animals

Hashimoto-Hill

Alenghat

2021

Front. Genet.

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“…2014, Martinez‐Medina & Garcia‐Gil 2014, Dogan et al . 2020). Eradication of intramucosal E. coli correlates with clinical response in dogs receiving fluoroquinolone therapy, which supports a causal role for E. coli in the pathogenesis of GC in dogs (Simpson et al .…”

Section: Introductionmentioning

confidence: 99%

Clinical characteristics and long‐term outcome of E. coli‐associated granulomatous ileocolitis in dogs: five cases (2010‐2014)

Cochran

Hill

Lotti³

et al. 2021

J of Small Animal Practice

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To describe the clinical characteristics and long-term outcome of Escherichia coli-associated granulomatous ileocolitis in dogs.MethOds: Retrospective review of medical records from dogs with periodic acid-Schiff positive (PAS+) granulomatous ileocolitis and mucosally invasive E. coli in the ileum and colon. Initial bacterial colonisation was evaluated using fluorescence in situ hybridization (FISH) in all dogs and corroborated with colonic and/or ileal culture, when performed.Results: Four boxer dogs and 1 French Bulldog with PAS+ granulomatous ileocolitis (GIC) were evaluated. All dogs had chronic diarrhoea refractory to empirical therapy. Ileocolonoscopy revealed mucosal haemorrhage and ulceration in the ileum (3/4) and colon (5/5). E. coli were visualised as clusters within the ileal and colonic mucosa. Complete (CR, 4/5) or partial (PR, 1/5) clinical response to fluoroquinolones was noted in all dogs within 30 days. CR was sustained in three of four dogs (median disease-free interval 40 months, range 16 to 60). Two dogs relapsed while receiving fluoroquinolones.Repeat biopsy isolated multidrug-resistant, mucosally invasive E. coli in the ileum (1/2) and colon (2/2). Targeted antimicrobial therapy was associated with long-term PR (78 months) in both dogs. clinical significance: Concurrent E. coli-associated granulomatous inflammation in the ileum and colon did not impart a poor clinical outcome or lack of response to the conventional standard of care for granulomatous colitis in dogs that were aggressively diagnosed and treated. Clinical outcome was influenced by antimicrobial resistance, with response dependent upon antimicrobial therapy informed by susceptibility testing.

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“…2,3,14,39,40 We found that antimicrobial treatment guided by susceptibility dogs, lending credence to this form of GC being host-rather than pathogen-driven. 59 We also explored the molecular mechanisms underlying FQ-R.…”

Section: Discussionmentioning

confidence: 99%

Escherichia coli‐associated granulomatous colitis in dogs treated according to antimicrobial susceptibility profiling

Manchester

Dogan

Guo

et al. 2020

Veterinary Internal Medicne

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Background: Eradication of intramucosal Escherichia coli correlates with remission of periodic acid-Schiff-positive E coli-associated granulomatous colitis (GC). Treatment failures attributed to multidrug resistant (MDR) bacteria necessitate alternative approaches. Hypothesis/objectives: Determine clinical outcome of E coli-associated GC in dogs treated based on antimicrobial susceptibility profiling and characterize E coli phylogeny and resistance mechanisms. Animals: Twenty Boxers and 4 French Bulldogs with E coli-associated GC. Methods: Culture, antimicrobial susceptibility profiling, and molecular characterization of E coli were performed and response to treatment was evaluated. Results: Initial biopsy sample culture yielded fluoroquinolone-sensitive (FQ-S) E coli from 9/24 dogs and fluoroquinolone-resistant (FQ-R) E coli from 15/24. All but 1 FQ-R E coli were MDR with susceptibility to macrophage-penetrating antimicrobials restricted to carbapenems in 13/15 dogs. Of 22/24 treated based on susceptibility profiling, 8/9 FQ-S dogs had complete initial clinical response (CR) during fluoroquinolone (FQ) treatment, whereas 9/13 FQ-R dogs had complete or partial response (PR) during meropenem or doxycycline treatment. In 5/9 FQ-S and 12/13 FQ-R dogs with follow-up ≥3 months, CR was sustained in 5/5 FQ-S (median, 25 months; range, 4-46) whereas 6/12 FQ-R had long-term CR (median, 59 months; range 15-102), 4/12 PR (median, 19 months; range, 5-65), and 2/12 had no response (NR). Four dogs with long-term follow-up died within 4 years of diagnosis, including 2 euthanized for refractory colitis. Escherichia coli were genetically diverse. Fluoroquinolone resistance was associated with mutations in gyrA and parC, with plasmid-mediated resistance less common. Conclusions and Clinical Importance: Antimicrobial treatment guided by susceptibility profiling was associated with positive long-term outcomes in >80% of cases.

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Molecular and Phenotypic Characterization of Escherichia coli Associated with Granulomatous Colitis of Boxer Dogs

Cited by 17 publications

References 59 publications

Inflammation-Associated Microbiota Composition Across Domestic Animals

Inflammation-Associated Microbiota Composition Across Domestic Animals

Clinical characteristics and long‐term outcome of E. coli‐associated granulomatous ileocolitis in dogs: five cases (2010‐2014)

Escherichia coli‐associated granulomatous colitis in dogs treated according to antimicrobial susceptibility profiling

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