Concurrent infections by all four dengue virus serotypes during an outbreak of dengue in 2006 in Delhi, India

Bharaj, Preeti; Chahar, Harendra Singh; Pandey, Avinash; Diddi, Kavita; Dar, Lalit; Guleria, Randeep; Kabra, S. K.; Broor, Shobha

doi:10.1186/1743-422x-5-1

Cited by 251 publications

(219 citation statements)

References 23 publications

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“…Concurrent infection with the two different serotypes, DENV-1 and DENV-2, was identified in 43% of the samples in the present study. Similarly 43% co-infection was reported from Davangere, Karnataka [5] and 57% from Thiruvananthapuram, Kerala [6], 32·5% from Moreh, Manipur [38] and 19% from Delhi [3]. Our earlier work reported 9% co-infections during 2011 [7], 14% during 2013 [2] and 18% during 4 years of study from 2011-14 [39] in Delhi.…”

Section: Discussionsupporting

confidence: 63%

“…Mean days of illness in co-infected patients was 3·80 (±1·72) days. The platelet count of the coinfected patients ranged from 0·6 to 3·5 lac/mm 3 . Three patients showed severe disease with mild haemorrhagic tendencies.…”

Section: Characteristics Of Co-infected Vs Mono-infected Patientsmentioning

confidence: 98%

“…At the same time the mono-infected patients also showed similar clinical symptoms with fever being the most common symptom. The platelet count of mono-infected patients ranged from 1·8 to 3·7 lac/mm 3 . None of the mono-infected patient had haemorrhagic tendencies.…”

Section: Characteristics Of Co-infected Vs Mono-infected Patientsmentioning

confidence: 99%

“…Recent studies have reported co-circulation of multiple serotypes in different geographical regions of the world including India [1][2][3]. In addition, concurrent infections with more than one serotype of dengue virus have been documented in various studies [2,[4][5][6].…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of co-infection with dengue viruses during 2014 in New Delhi, India

et al. 2016

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SUMMARYDengue fever is an arthropod-borne viral infection that has become endemic in several parts of India including Delhi. We studied occurrence of co-infection with dengue viruses during an outbreak in New Delhi, India in 2014. For the present study, blood samples collected from symptomatic patients were analysed by RT-PCR. Eighty percent of the samples were positive for dengue virus. The result showed that DENV-1 (77%) was the predominant serotype followed by DENV-2 (60%). Concurrent infection with more than one serotype was identified in 43% of the positive samples. Phylogenetic analysis clustered DENV-1 strains with the American African and DENV-2 strains in Cosmopolitan genotypes. Four common amino-acid mutations were identified in the envelope gene of DENV-1 sequences (F337I, A369T, V380I and L402F) and one common mutation (N390S) in the DENV-2 sequences. Further analysis revealed purifying selection in both the serotypes. A significant number of patients were co-infected with DENV-1 and DENV-2 serotypes. Although we do not have direct evidence to demonstrate co-evolution of these two stereotypes, nonetheless their simultaneous occurrence does indicate that they are favoured by evolutionary forces. An ongoing surveillance and careful analysis of future outbreaks will strengthen the concept of co-evolution or otherwise. Whether the concurrent dengue viral infection is correlated with disease severity in a given population is another aspect to be pursued. This study is envisaged to be useful for future reference in the context of overall epidemiology.

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Section: Discussionsupporting

confidence: 63%

Section: Characteristics Of Co-infected Vs Mono-infected Patientsmentioning

confidence: 98%

Section: Characteristics Of Co-infected Vs Mono-infected Patientsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Occurrence of co-infection with dengue viruses during 2014 in New Delhi, India

et al. 2016

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“…The major serotype reported from PGI, Chandigarh during the outbreak in the year 2007 was serotype-1, but in 2005 it was serotype-2 [11]. A study from Delhi had reported concurrent prevalence of all the serotypes during 2008 [19]. Major dengue epidemics have been observed in the Indian subcontinent since the 1980s & researchers from the Pune-based NIV found that Indian DENV-2 strains isolated from 1956 to 2005 belonged to only two of the six genotypesAmerican and Cosmopolitan [20].…”

mentioning

confidence: 99%

NS1 Antigen Capture ELISA an Effective Method for Diagnosis of Early Dengue Infection - Report of an Outbreak at Angul District, Odisha, India

Mahapatra

Sarangi

Mahapatra

et al. 2014

JCDR

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Clinical applicability of single‐tube multiplex reverse‐transcriptase PCR in dengue virus diagnosis and serotyping

Mishra¹,

Sharma²,

Pujhari³

et al. 2011

Clinical Laboratory Analysis

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This study has evaluated the clinical applicability of a single-tube multiplex RT-PCR as compared with a two-step nested RT-PCR for the diagnosis as well as serotyping of dengue virus in patient's samples. Seventy-six acute phase blood samples collected from clinically suspected dengue patients during the 2008 outbreak were subjected to two-step nested RT-PCR and single-tube multiplex RT-PCR for dengue diagnosis and serotyping. Of the 76 samples, 17 (22.4%) were positive for dengue viral RNA. Single dengue virus infection was found in 16 cases and 1 had concurrent infection with two serotypes (3&1). Dengue serotype 3 was the predominant serotype (70.5%), followed by serotype 1 (23.5%). Single-tube multiplex PCR had concordant result with that of two-step nested RT-PCR including the one with concomitant infection. This study reveals the predominance of dengue serotype 3 in North India in addition to the co-circulation of multiple serotypes and concomitant infection. The rapid and accurate diagnostic capability of single-tube multiplex RT-PCR used in the study appears to be promising enough to be commonly used for dengue viral detection as well as serotyping.

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Concurrent infections by all four dengue virus serotypes during an outbreak of dengue in 2006 in Delhi, India

Cited by 251 publications

References 23 publications

Occurrence of co-infection with dengue viruses during 2014 in New Delhi, India

Occurrence of co-infection with dengue viruses during 2014 in New Delhi, India

NS1 Antigen Capture ELISA an Effective Method for Diagnosis of Early Dengue Infection - Report of an Outbreak at Angul District, Odisha, India

Clinical applicability of single‐tube multiplex reverse‐transcriptase PCR in dengue virus diagnosis and serotyping

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