Concurrent Synthesis and Degradation of Alcohol Dehydrogenase in Elicitor-Treated and Wounded Potato Tubers

Constabel, C. Peter; Matton, Daniel P.; Brisson, Normand

doi:10.1104/pp.94.3.887

Cited by 9 publications

(4 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We therefore monitored other proteins in the same extracts by using antisera directed against alcohol dehydrogenase, 5-1ipoxygenase, and starch phosphorylase. Alcohol dehydrogenase, a constitutive tuber protein but whose synthesis is further stimulated by elicitor treatment (Constabel et al 1990), disappeared at the same time points as STH-2 protein (Fig. 4, upper panel).…”

Section: Resultsmentioning

confidence: 89%

“…4 does show that not all proteins were affected the same way. Previously we had shown that synthesis of alcohol dehydrogenase is induced during the defense response of potato tuber disks (Constabel et al 1990). The fact that alcohol dehydrogenase also disappeared from western blots whereas 5-1ip-oxygenase, the 18-kDa protein, and the majority of Coomassie-stained bands remained at relatively constant levels indicates that perhaps actively synthesized proteins are most susceptible to this degradation.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The defense-related STH-2 gene product of potato shows race-specific accumulation after inoculation with low concentrations of Phytophthora infestans zoospores

Brisson

1992

Planta

Self Cite

View full text Add to dashboard Cite

The defense-related STH-2 gene is rapidly activated following infection or elicitor treatment of potato (Solanum tuberosum L.) tubers. However, its physiological or biochemical function is unknown. To study the STH-2 gene product and its accumulation during the defense response, we raised antibodies to a β-galactosidase-STH-2 fusion protein in Escherichia coli. The antiserum specifically recognized a protein of the predicted 17-kDa size in extracts of elicited tuber disks when analyzed by Western blot. In control extracts this band was not detected. The accumulation of STH-2 protein in response to incompatible and compatible zoospores of Phytophthora infestans (Mont.) de Bary depended on the inoculum density applied. Whereas a low concentration of spores induced accumulation of STH-2 protein faster in the incompatible than the compatible interaction, this difference in timing was less pronounced at higher inoculum densities. Inoculation with a high concentration of compatible spores also resulted in the disappearance of STH-2 protein late during the infection. In both control and induced tuber tissue the antibody strongly reacted with an unknown protein of 18 kDa. This protein was present constitutively in tubers, but in leaves its accumulation was stimulated by inoculation with P. infestans.

show abstract

Section: Resultsmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

The defense-related STH-2 gene product of potato shows race-specific accumulation after inoculation with low concentrations of Phytophthora infestans zoospores

Brisson

1992

Planta

Self Cite

View full text Add to dashboard Cite

show abstract

“…Induction of ADH may occur as a result of such signals but is probably more likely to represent a classic response to a nominal 'anaerobic' state which exists for a sufficiently long time window [ 1,10,12,26,30]. ADH is one of a number of enzymes involved in glycolysis and fermentation which are induced in response to a low partial pressure of 02, i.e.…”

Section: Discussionmentioning

confidence: 99%

Rapid changes in oxidative metabolism as a consequence of elicitor treatment of suspension-cultured cells of French bean (Phaseolus vulgaris L.)

et al. 1995

View full text Add to dashboard Cite

Stressed plant cells often show increased oxygen uptake which can manifest itself in the transient production of active oxygen species, the oxidative burst. There is a lack of information on the redox status of cells during the early stages of biotic stress. In this paper we measure oxygen uptake and the levels of redox intermediates NAD/NADH and ATP and show the transient induction of the marker enzyme for redox stress, alcohol dehydrogenase. Rapid changes in the redox potential of elicitor-treated suspension cultures of French bean cells indicate that, paradoxically, during the period of maximum oxygen uptake the levels of ATP and the NADH/NAD ratio fall in a way that indicates the occurrence of stress in oxidative metabolism. This period coincides with the maximum production of active oxygen species particularly H2O2. The cells recover and start producing ATP immediately of H2O2 production. This indicates that the increased O2 uptake is primarily incorporated into active O2 species. A second consequence of these changes is probably a transient compromising of the respiratory status of the cells as indicated in expression of alcohol dehydrogenase. Elicitor-induced bean ADH was purified to homogeneity and the M(r) 40,000 polypeptide was subjected to amino acid sequencing. 15% of the whole protein was sequenced from three peptides and was found to have nearly 100% sequence similarity to the amino acid sequence for pea ADH1 (PSADH1). The cDNA coding for the pea enzyme was used to demonstrate the transient induction of ADH mRNA in elicitor-treated bean cells. Enzyme activity levels also increased transiently subsequently. Increased oxygen uptake has previously been thought to be associated with provision of energy for the changes in biosynthesis that occur rapidly after perception of the stress signal. However the present work shows that this rapid increase in oxygen uptake as a consequence of elicitor action is not wholly associated with respiration.

show abstract

“…How it is controlled and mediated is unclear, but the resulting zone of dead cells is thought to inhibit further growth of the pathogen [34]. This response is also accompanied by changes in various metabolic activities, including the synthesis of sesquiterpenoid phytoalexins [8], chitinase and t-l,3 glucanases [22] as well as phenylalanine ammonia-lyase, 4-coumarate:CoA ligase [15] and alcohol dehydrogenase [7,31]. Many genes coding for proteins of unknown function are also induced in potato upon infection, elicitor treatment or wounding [25,41,43].…”

Section: Introductionmentioning

confidence: 99%

Identification of cis-acting elements involved in the regulation of the pathogenesis-related gene STH-2 in potato

et al. 1993

Self Cite

View full text Add to dashboard Cite

We have characterized a genomic clone containing the potato pathogenesis-related genes STH-2 and STH-21. The two genes are found 4 kb apart on the same chromosome and their sequences are highly similar. They present the same transcriptional orientation and are both interrupted by a single intron. A chimaeric gene consisting of 1015 bp of 5'-flanking sequence and part of the first exon of STH-2 fused to the bacterial beta-glucuronidase gene was highly-expressed in tubers of transgenic potato plants after wounding and elicitor treatments. The levels of activity observed in these transgenic plants parallel those observed for the accumulation of STH-2 mRNAs under similar conditions. This indicates that cis-acting elements necessary for the proper activation of the gene are present within 1 kb of 5'-flanking sequences. Functional analysis of 5' deletions of the STH-2/GUS constructs by transient expression in leaf protoplasts revealed the presence of an upstream regulatory sequence between -135 and -52 which contains a TGAC motif, and a possible negative regulatory region between -52 and -28. A factor present in nuclear extracts of wounded potato tubers was found to bind specifically to nucleotides located between -135 to -105, suggesting that this region contains important cis-regulatory elements.

show abstract

Concurrent Synthesis and Degradation of Alcohol Dehydrogenase in Elicitor-Treated and Wounded Potato Tubers

Abstract: The accumulation of alcohol dehydrogenase (ADH) in arachidonic acid-elicited potato (Solanum tuberosum L.) tuber discs was studied. In accordance with our previous report of the ac

Cited by 9 publications

References 23 publications

The defense-related STH-2 gene product of potato shows race-specific accumulation after inoculation with low concentrations of Phytophthora infestans zoospores

The defense-related STH-2 gene product of potato shows race-specific accumulation after inoculation with low concentrations of Phytophthora infestans zoospores

Rapid changes in oxidative metabolism as a consequence of elicitor treatment of suspension-cultured cells of French bean (Phaseolus vulgaris L.)

Identification of cis-acting elements involved in the regulation of the pathogenesis-related gene STH-2 in potato

Contact Info

Product

Resources

About