Conditioned media from human osteoarthritic synovium induces inflammation in a synoviocyte cell line

Belluzzi, Elisa; Olivotto, Eleonora; Toso, Giovanna; Cigolotti, Augusto; Pozzuoli, Assunta; Biz, Carlo; Trisolino, Giovanni; Ruggieri, Pietro; Grigolo, Brunella; Ramonda, Roberta; Favero, Marta

doi:10.1080/03008207.2018.1470167

Cited by 59 publications

(56 citation statements)

References 48 publications

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“…Favero et al observed the elevation of CCL21 and CCL5 in coculture of meniscus and synovial membrane from patients with osteoarthritis [47]. Conditioned media from osteoarthritis tissues like cartilage, IFP, meniscus, and synovium induced and enhanced production of CXCL8 and CCL21 in the synoviocyte cell line [67]. Several other studies showed an enhanced CCL2 level in patients with osteoarthritis [47,63,68].…”

Section: The Role Of Chemokines In Osteoarthritismentioning

confidence: 97%

The Role of Inflammation in the Pathogenesis of Osteoarthritis

Chow

Chin

2020

Mediators of Inflammation

361

221

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A joint is the point of connection between two bones in our body. Inflammation of the joint leads to several diseases, including osteoarthritis, which is the concern of this review. Osteoarthritis is a common chronic debilitating joint disease mainly affecting the elderly. Several studies showed that inflammation triggered by factors like biomechanical stress is involved in the development of osteoarthritis. This stimulates the release of early-stage inflammatory cytokines like interleukin-1 beta (IL-1β), which in turn induces the activation of signaling pathways, such as nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), phosphoinositide 3-kinase/protein kinase B (PI3K/AKT), and mitogen-activated protein kinase (MAPK). These events, in turn, generate more inflammatory molecules. Subsequently, collagenase like matrix metalloproteinases-13 (MMP-13) will degrade the extracellular matrix. As a result, anatomical and physiological functions of the joint are altered. This review is aimed at summarizing the previous studies highlighting the involvement of inflammation in the pathogenesis of osteoarthritis.

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Section: The Role Of Chemokines In Osteoarthritismentioning

confidence: 97%

The Role of Inflammation in the Pathogenesis of Osteoarthritis

Chow

Chin

2020

Mediators of Inflammation

361

221

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“…In addition to its involvement in early stages of the disease, synovial inflammation is also thought to be secondary to penetration of osteocartilaginous fragments and inflammatory/catabolic factors in the synovial cavity [21]. Interestingly, all OA joint tissues are able to stimulate synoviocytes and might be involved in the progression of OA synovitis [17].…”

Section: The Role Of the Ifp-synovial Membrane As A Functional Unimentioning

confidence: 99%

“…In this context, careful attention should be paid to a new emerging concept reconsidering the relation between the IFP and the synovial membrane, which are tissues innervated and well vascularized [15, 16]. Recently, it has also been shown that IFP in OA represents a source of inflammatory molecules and is more inflamed and vascularized compared to nonosteoarthritic controls (Figure 1) [17–19]. Moreover, it has been known that IFP can be the cause of anterior knee pain [20].…”

Section: Introductionmentioning

confidence: 99%

Contribution of Infrapatellar Fat Pad and Synovial Membrane to Knee Osteoarthritis Pain

Belluzzi

Stocco

Pozzuoli

et al. 2019

BioMed Research International

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141

124

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Osteoarthritis (OA) is the most common form of joint disease and a major cause of pain and disability in the adult population. Interestingly, there are patients with symptomatic OA displaying pain, while patients with asymptomatic OA that do not experience pain but show radiographic signs of joint damage. Pain is a complex experience integrating sensory, affective, and cognitive processes related to several peripheral and central nociceptive factors besides inflammation. During the last years, the role of infrapatellar fat pad (IFP), other than the synovial membrane, has been investigated as a potential source of pain in OA. Interestingly, new findings suggest that IFP and synovial membrane might act as a functional unit in OA pathogenesis and pain. The present review discuss the role of IFP and synovial membrane in the development of OA, with a particular focus on pain onset and the possible involved mediators that may play a role in OA pathology and pain mechanisms. Inflammation of IFP and synovial membrane may drive peripheral and central sensitization in KOA. Since sensitization is associated with pain severity in knee OA and may potentially contribute to the transition from acute to chronic, persistent pain in knee OA, preventing sensitization would be a potentially effective and novel means of preventing worsening of pain in knee OA.

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“…Recently, attention has focused on the role of the IFP in OA pathophysiology [3,10,11]. It has been shown that IFP produces pro-inflammatory mediators inducing synovial inflammation and seems to act as an anatomo-functional unit with synovial membrane contributing to OA onset and progression [3,[10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Infrapatellar Fat Pad Gene Expression and Protein Production in Patients with and without Osteoarthritis

Belluzzi

Macchi

Fontanella

et al. 2020

IJMS

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Osteoarthritis (OA) is one of the most common joint disorders. Evidence suggests that the infrapatellar fat pad (IFP) is directly involved in OA pathology. However, a comparison between OA versus non-OA IFP is still missing. Thus, the aim of this study was to compare IFP molecular, adipocytes and extracellular matrix characteristics of patients affected by OA, and patients undergoing anterior cruciate ligament (ACL) reconstruction. We hypothesized that not only inflammation but also changes in adipocytes and extracellular matrix (ECM) composition might be involved in OA pathogenesis. Fifty-three patients were enrolled. IFP biopsies were obtained, evaluating: (a) lymphocytic infiltration and vascularization; (b) adipocytes area and number; (c) adipo-cytokines and extracellular matrix gene expression levels; (d) IL-6 and VEGF protein production; (e) collagen fibers distribution. OA IFP was more inflamed and vascularized compared to ACL IFP. OA IFP adipocytes were larger and numerically lower (1.3-fold) than ACL IFP adipocytes. An increase of gene expression of typical white adipose tissue genes was observed in OA compared to ACL IFP. Collagen-types distribution was different in the OA IFP group compared to controls, possibly explaining the change of the biomechanical characteristics found in OA IFP. Statistical linear models revealed that the adipocyte area correlated with BMI in the OA group. In conclusion, inflammation and fibrotic changes of OA IFP could represent novel therapeutic targets to counteract OA.

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Conditioned media from human osteoarthritic synovium induces inflammation in a synoviocyte cell line

Cited by 59 publications

References 48 publications

The Role of Inflammation in the Pathogenesis of Osteoarthritis

The Role of Inflammation in the Pathogenesis of Osteoarthritis

Contribution of Infrapatellar Fat Pad and Synovial Membrane to Knee Osteoarthritis Pain

Infrapatellar Fat Pad Gene Expression and Protein Production in Patients with and without Osteoarthritis

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