Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters

Mahiddine, Feriel Yasmine; Kim, Jin Wook; Qamar, Ahmad Yar; Chang-hyun, Jeong; Kim, Soo Hyun; Jung, Eun Joong; Kim, Min Jung

doi:10.3390/ani10101899

Cited by 20 publications

(15 citation statements)

References 96 publications

(121 reference statements)

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“…Consistent with our ndings, applications of Sertoli cell-CM on the azoospermia model induced spermatogenesis in mice (Panahi et al, 2020). In addition, 10% of the CM from canine amniotic membrane-derived MSCs enhanced the quality of frozen-thawed sperm cells (Mahiddine et al, 2020). Since EVs of MSCs contains cell paracrine factors (Yuan et al, 2020), the application of BMCM for azoospermia clinical studies seems to be promising.…”

Section: Discussionsupporting

confidence: 85%

“…Epidermal growth factor (EGF), broblast growth factor 2/basic broblast growth factor (FGF-2/bFGF), hepatocyte growth factor (HGF), insulin-like growth factor I (IGF-I), insulin-like growth factor II (IGF-II), platelet-derived growth factor (PDGF) and vascular endothelial-derived growth factor (VEGF) are the important growth factors in the CM of MSCs (Noverina et al, 2019). Furthermore, the presence of antioxidants in the CM (Mahiddine et al, 2020), in combination with cytokines and growth factors may have an effect on spermatogenesis of azoospermia mice.…”

Section: Discussionmentioning

confidence: 99%

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Restoration of spermatogenesis in azoospermic mice by bone marrow mesenchymal stromal/stem cells conditioned medium

Zhankina

Afshar

Farrar

et al. 2021

Preprint

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One of the main cause of male infertility is non-obstructive azoospermia, which is not manageable medically. The first aim of the current research was to show the effect of extracellular vesicle-contained conditioned media (CM) instead of mesenchymal stromal/stem cells (MSCs) for treatment of non-obstructive azoospermia. In the next step, we aimed to study the differentiation potential of MSCs into spermatocytes after injection of MSCs in mice seminiferous tubules. This study has provided an applied treatment for busulfan-induced azoospermia using adipose tissue-derived (AT-MSCs) and bone marrow-derived MSCs (BM-MSCs) and bone marrow CM (BMCM) in animal models. In this regard, 30 male adult Balb/C mice (30±5g) and two female eGFP+/+ Balb/C mice (30±5g) were used to design experimental groups and to culture stem cells, respectively. Then, six groups including intact control, azoospermia, AT-MSC therapy, BM-MSC therapy, BMCM therapy, and spontaneous healing groups were considered. All groups except intact control were induced azoospermia by injecting two doses of busulfan (10 mg/kg) with 21 days’ interval. Testes of all mice were removed and studied through histomorphometry and flow cytometry analysis 60 days after treatment. Histomorphometry and flow cytometry evaluation of testes showed normal morphology of most of the seminiferous tubules of therapy groups as well as successful recovery of spermatogenesis, but spermatogenesis was not observed in the azoospermia group. It is worth notable that the results of the BM-MSC therapy group were more favorable than other therapy groups. Consequently, AT-MSC, BM-MSC and BMCM can be strongly suggested as candidates in the therapy of azoospermia.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Restoration of spermatogenesis in azoospermic mice by bone marrow mesenchymal stromal/stem cells conditioned medium

Zhankina

Afshar

Farrar

et al. 2021

Preprint

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show abstract

“…Consistent with our ndings, applications of Sertoli cell-CM on the azoospermia model induced spermatogenesis in mice (33). In addition, 10% of the CM from canine amniotic membrane-derived MSCs enhanced the quality of frozen-thawed sperm cells (34). Since EVs of MSCs contains cell paracrine factors (35), the application of BMCM for azoospermia clinical studies seems to be promising.…”

Section: Discussionsupporting

confidence: 77%

“…Epidermal growth factor (EGF), broblast growth factor 2/basic broblast growth factor (FGF-2/bFGF), hepatocyte growth factor (HGF), insulin-like growth factor I (IGF-I), insulin-like growth factor II (IGF-II), platelet-derived growth factor (PDGF) and vascular endothelial-derived growth factor (VEGF) are the important growth factors in the CM of MSCs (38). Furthermore, the presence of antioxidants in the CM (34), in combination with cytokines and growth factors may have an effect on spermatogenesis of azoospermia mice.…”

Section: Discussionmentioning

confidence: 99%

Restoration of spermatogenesis in azoospermic mice by bone marrow mesenchymal stromal/stem cells conditioned medium

Zhankina

Afshar

Farrar

et al. 2022

Preprint

View full text Add to dashboard Cite

Background One of the main cause of male infertility is non-obstructive azoospermia, which is not manageable medically. The first aim of the current research was to show the effect of extracellular vesicle-contained conditioned media (CM) instead of mesenchymal stromal/stem cells (MSCs) for treatment of non-obstructive azoospermia. In the next step, we aimed to study the differentiation potential of MSCs into spermatocytes after injection of MSCs in mice seminiferous tubules. This study has provided an applied treatment for busulfan-induced azoospermia using adipose tissue-derived (AT-MSCs) and bone marrow-derived MSCs (BM-MSCs) and bone marrow CM (BMCM) in animal models. Methods Thirty male adult Balb/C mice (30±5 g) and two female eGFP+/+Balb/C mice (30±5 g) were used to design experimental groups and to culture stem cells, respectively. Then, six groups including intact control, azoospermia, AT-MSC therapy, BM-MSC therapy, BMCM therapy, and spontaneous healing groups were considered. All groups except intact control were induced azoospermia by injecting two doses of busulfan (10 mg/kg) with 21 days’ interval. Testes of all mice were removed and studied through histomorphometry and flow cytometry analysis 60 days after treatment. Results Histomorphometry and flow cytometry evaluation of testes showed normal morphology of most of the seminiferous tubules of therapy groups as well as successful recovery of spermatogenesis, but spermatogenesis was not observed in the azoospermia group. It is worth notable that the results of the BM-MSC therapy group were more favorable than other therapy groups. Conclusions AT-MSC, BM-MSC and BMCM can be strongly suggested as candidates in the therapy of azoospermia.

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“…The results are expressed as the percentage of live sperm cells (12) (Table 2) and showed no difference between orchiopexy dogs and control dogs. The sperm acrosome membrane was analyzed using fluorescein isothiocyanate-conjugated peanut agglutinin (FITC-PNA), as previously described (13). In brief, semen was smeared on glass slides, air-dried, fixed in absolute methanol, stained, and mounted with anti-fade mounting medium (VECTASHIELD R , Vector Laboratories, Burlingame, CA, USA).…”

Section: Case Presentationmentioning

confidence: 99%

Case Report: Orchiopexy in Two Poodle Dogs and Its Effect on Their Sperm Quality Parameters

Mahiddine¹,

Kim²

2021

Front. Vet. Sci.

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Cryptorchidism is a common congenital abnormality encountered in veterinary clinics. The treatment of choice for this condition is a surgical procedure named orchiectomy or orchidectomy, where the retained testicle is removed. Surgical placement and fixation of the cryptorchid testicle into the scrotum, referred to as orchiopexy, is used in humans. However, due to the hereditary nature of cryptorchidism in dogs, this treatment option has not been proposed in veterinary clinics. Two adult Poodle dogs were referred to our research facility for a sperm parameter evaluation check. The two dogs were unilateral cryptorchid dogs treated with orchiopexy before the age of 6 months. Their sperm kinematics and morphology were within normal ranges, and their libido and testicles sizes were normal. Treatment of unilateral cryptorchidism by orchiopexy in dogs before the age of 6 months successfully restored spermatogenic function and sperm quality-related parameters. However, due to the nature of this condition, orchiectomy remains the treatment of choice.

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Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters

Cited by 20 publications

References 96 publications

Restoration of spermatogenesis in azoospermic mice by bone marrow mesenchymal stromal/stem cells conditioned medium

Restoration of spermatogenesis in azoospermic mice by bone marrow mesenchymal stromal/stem cells conditioned medium

Restoration of spermatogenesis in azoospermic mice by bone marrow mesenchymal stromal/stem cells conditioned medium

Case Report: Orchiopexy in Two Poodle Dogs and Its Effect on Their Sperm Quality Parameters

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