Conditions of Aerogenic Exposure to Benzene and Genetic Status as Factors of Formation of Immune Profile Features in Men with Autonomic Regulation Disturbances

Dolgikh, O.V.; Zaitseva, Nina V.; Nikonoshina, N. A.

doi:10.1007/s10517-021-05199-z

Cited by 2 publications

(1 citation statement)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immunosuppression induced by increased FOXP3 expression has been suggested to be associated with hematopoietic toxicity induced by sub-acute exposure to benzene, 42 while the inactivation of immunosuppressive function induced by inhibition of FOXP3 gene function has been reported to be associated with the increased sensitivity to disturbances in autonomic nervous regulation in chronic benzene-exposure. 43 Since DNA methylation is a critical regulator of FOXP3 expression, it is reasonable to speculate that the different patterns of FOXP3 expression in response to environmental stimulation could be induced by changes in DNA methylation patterns. Identifying the patterns of FOXP3 methylation in response to environmental pollutants or pathological outcomes is necessary to ensure the biological plausibility of this epigenetic biomarker in the application of the risk assessment and to target it for the appropriate prevention strategies.…”

Section: Discussionmentioning

confidence: 99%

The hypermethylation of FOXP3 gene as an epigenetic marker for the identification of arsenic poisoning risk

Fang

Zhang

2022

Hum Exp Toxicol

View full text Add to dashboard Cite

Background and Purpose: Arsenic exposure can lead to skin lesions and multiple organ damage, which are not easily reversible and for which there is no effective therapeutics. Identification of reliable epigenetic markers is essential for early recognition of arsenic poisoning risk. Anomalous DNA methylation of immune homeostasis regulator FOXP3 is a critical mechanism for triggering arsenic poisoning. This study aims to explore the value of FOXP3 methylation in the identification of arsenic poisoning risk. Methods: 88 arsenic poisoning subjects and 41 references were recruited. Urinary arsenic contents and FOXP3 methylation in PBLCs was measured by ICP-MS and pyrosequencing, respectively. Results: The results showed that the elevated FOXP3 methylation in PBLCs were associated with the increased levels of urinary arsenic and were positively associated with the increased risk of arsenic poisoning and its progression. The result of mediation analysis revealed that 24.3% of the effect of arsenic exposure on the risk of arsenic poisoning was mediated by increased FOXP3 methylation. Additionally, we constructed a nomogram model with FOXP3 methylation as an epigenetic predictor to assess the probability of individual arsenic poisoning. The model showed a robust ability in the discrimination of arsenic poisoning risk, with an area under receiver operating characteristics curve of 0.897(0.845–0.949) and more than 70% accuracy. The calibration curves and the Harrell concordance index showed that the consistency rate between the probability predicted by the nomogram model and the actual probability is 89.7%. Conclusions: Taken together, we found the great potential of FOXP3 methylation for the identification of arsenic poisoning risk and provided a new approach to the application of epigenetic markers in accurately quantifying the risk of adverse outcomes.

show abstract

Section: Discussionmentioning

confidence: 99%

The hypermethylation of FOXP3 gene as an epigenetic marker for the identification of arsenic poisoning risk

Fang

Zhang

2022

Hum Exp Toxicol

View full text Add to dashboard Cite

show abstract

Polymorphism of genes of circadian rhythms in employees of a continuous production enterprise with a shift work schedule

Subbotina

Dolgikh

2023

Med. tr. prom. èkol.

View full text Add to dashboard Cite

The circadian system plays an important role in regulating the functions of the human body. As a result of violations of the circadian system, functional disorders such as sleep disorders, carbohydrate metabolism, metabolic syndrome turning into obesity, cardiovascular pathology, oncological processes occur. One of the socially significant causes of circadian rhythm disorders is a shift work schedule. The study aims to explore the genetic status of employees and identify markers of predisposition to the formation of "intolerance" of shift work. Researchers have examined two groups of employees of the enterprise: observation group — 40 people gave 23–63 years old (30 women, 10 men) with a shift work schedule, the comparison group consisted of 51 people 24–62 years old (41 women, 10 men) of the administrative apparatus with a daily work schedule. Typing of polymorphic loci of the circadian system genes was carried out by sets of the company "Syntol" (Russia) using the Real-Time PCR method on a CFX96 device with allelic discrimination of fluorescence curves. Genotyping of the loci of the five main circadian genes allowed us to establish that the occurrence of the minor allele T of the MTNR1A melatonin receptor gene (rs34532313) in continuous production workers is 30%, which is five times higher than in workers with a standardized work schedule (p<0.05). At the same time, workers with a shift schedule had a significant hypo-expression of serum serotonin (1.54 times, p<0.05) relative to the comparison group. Continuous production workers have an increased occurrence of the minor allele of the MTNR1A melatonin receptor T gene (rs34532313) with a simultaneous decrease in the expression of serotonin catecholamine, which in shift work conditions can affect the formation of dysregulatory disorders of the functioning of homeostatic systems and the course of physiological metabolic processes. As a result, the polymorphism of the MTNR1A gene (rs34532313) can use as a marker of "intolerance" to shift work. Limitations. There are quantitative restrictions on the observation group of persons of different genders. Ethics. When conducting the study, the authors were guided by the Ethical principles of conducting medical research with the participation of a person as a subject, set out in the Helsinki Declaration of the World Medical Association of the last revision. The patients signed a voluntary informed consent for the examination.

show abstract

Conditions of Aerogenic Exposure to Benzene and Genetic Status as Factors of Formation of Immune Profile Features in Men with Autonomic Regulation Disturbances

Cited by 2 publications

References 11 publications

The hypermethylation of FOXP3 gene as an epigenetic marker for the identification of arsenic poisoning risk

The hypermethylation of FOXP3 gene as an epigenetic marker for the identification of arsenic poisoning risk

Polymorphism of genes of circadian rhythms in employees of a continuous production enterprise with a shift work schedule

Contact Info

Product

Resources

About