Conductivity detection and quantitation of isotachophoretic analytes on a planar chip with on-line coupled separation channels

Masár, Marián; Žúborová, Mária; Bielčíková, J.; Kaniansky, Dušan; Jöhnck, Matthias; Stanislawski, Bernd

doi:10.1016/s0021-9673(00)01236-x

Cited by 45 publications

(34 citation statements)

References 23 publications

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“…The majority of recent research with microchip devices utilizes on-column contact conductivity detection [85][86][87][88][89][90][91][92][93][94][95][96]. In addition to being easy to fabricate, on-column has other advantages over an end-column arrangement.…”

Section: Figurementioning

confidence: 99%

“…Therefore, less band-broadening due to diffusion will occur, leading to a more efficient separation and better peak shapes. Also, several detectors can be located along the separation channel, making it possible to monitor the analyte zones at various locations along the channel [85][86][87][88]92]. An example of these devices is shown in Fig.…”

Section: Figurementioning

confidence: 99%

“…ITP [89][90][91][92] has also been used with conductivity detection as a separation technique either alone or in conjunction with CZE [86,88]. This technique has been used for a variety of applications including the isolation of the drug valproic acid from human serum [87].…”

Section: Figure 12mentioning

confidence: 99%

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Recent developments in electrochemical detection for microchip capillary electrophoresis

et al. 2004

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Significant progress in the development of miniaturized microfluidic systems has occurred since their inception over a decade ago. This is primarily due to the numerous advantages of microchip analysis, including the ability to analyze minute samples, speed of analysis, reduced cost and waste, and portability. This review focuses on recent developments in integrating electrochemical (EC) detection with microchip capillary electrophoresis (CE). These detection modes include amperometry, conductimetry, and potentiometry. EC detection is ideal for use with microchip CE systems because it can be easily miniaturized with no diminution in analytical performance. Advances in microchip format, electrode material and design, decoupling of the detector from the separation field, and integration of sample preparation, separation, and detection on-chip are discussed. Microchip CEEC applications for enzyme/immunoassays, clinical and environmental assays, as well as the detection of neurotransmitters are also described.

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Section: Figurementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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Recent developments in electrochemical detection for microchip capillary electrophoresis

et al. 2004

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“…While most of this work relies on CZE, isotachophoretic approaches have been described as well [8,9]. Further examples for quantitative microchip CE (MCE) include organic molecules like oxalate in urine [10], carnitines in water [11], 4-amino-3-methyl-N-ethyl-N-(b-methane sulfonamidoethyl)aniline in photographic developer [12], levoglucosan in aerosols [13], thiols in nerve agent degradation products [14], and homocysteine in plasma [15].…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of thiols in consumer products on a microfluidic CE chip with fluorescence detection

2007

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A microchip CE-based method for the quantification of the thiols mercaptoethanoic acid (MAA) and 2-mercaptopropionic acid (2-MPA) in depilatory cream and cold wave lotions was developed. The thiols were first derivatized with the fluorogenic reagent ammonium-7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD-F). The derivatives were separated within only 20 s by microchip CE and detected by their fluorescence. Conventional CE with diode array detection and LC with fluorescence detection were used for validation. The internal standard 3-mercaptopropionic acid (3-MPA) provided RSDs of multiple injections of only 4% or less for the MCE approach. LOD is 2 mM, LOQ 6 mM, and the linear range comprises nearly three decades of concentration starting at the LOQ.

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“…The microchip has two separation channels in a coupling arrangement, which allows a two-step separation to be performed on a single chip. The chip can also be used for zone electrophoresis (ZE) or ITP alone or for the combination ITP-ITP as well as ITP-ZE [25,26]. There are several recent publications available on diverse analyses on a chip similar to the one used in these experiments [23,[27][28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Isotachophoresis of β‐blockers in a capillary and on a poly(methyl methacrylate) chip

et al. 2004

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Analysis of the beta-blockers oxprenolol, atenolol, timolol, propranolol, metoprolol, and acebutolol in human urine by a combination of isotachophoresis (ITP) and zone electrophoresis (ZE) was investigated. Methods were developed with a conventional capillary electrophoresis (CE) apparatus and a poly(methyl methacrylate) (PMMA) microchip system. With CE the separation of oxprenolol, atenolol, timolol, and acebutolol from a standard solution containing 5 microg/mL of each compound was accomplished by performing ZE with transient ITP. The electrolyte system consisted of 10 mM sodium morpholinoethane sulfonate (pH 5.5) and 0.1% methylhydroxyethylcellulose as the leading electrolyte and 30 mM ortho-phosphoric acid (pH 2.0) as both the terminating and the ZE background electrolyte. With the microchip system the separation of oxprenolol and acebutolol from a standard solution containing 10 microg/mL of each compound was accomplished by a coupled-channel ITP-ZE device using the same leading electrolyte solution as the CE system but 5 mM glutamic acid (pH 3.4) as terminating and background electrolytes. The systems were used for analyses of patient urine samples. Water-soluble hydrophilic matrix compounds were removed from the urine samples by solid-phase extraction (SPE). Limits of quantification below 5 microg/mL could be achieved. The PMMA ITP-ZE chip has not earlier been used for analyses of any drugs from urine samples.

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Conductivity detection and quantitation of isotachophoretic analytes on a planar chip with on-line coupled separation channels

Cited by 45 publications

References 23 publications

Recent developments in electrochemical detection for microchip capillary electrophoresis

Recent developments in electrochemical detection for microchip capillary electrophoresis

Quantitative analysis of thiols in consumer products on a microfluidic CE chip with fluorescence detection

Isotachophoresis of β‐blockers in a capillary and on a poly(methyl methacrylate) chip

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