Conferring resistance to geminiviruses with the CRISPR–Cas prokaryotic immune system

Baltes, Nicholas J.; Hummel, Aaron W.; Konečná, Eva; Čegan, Radim; Bruns, Aaron N.; Bisaro, David M.; Voytas, Daniel F.

doi:10.1038/nplants.2015.145

Cited by 374 publications

(230 citation statements)

References 15 publications

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“…Indeed, biolistic co-transformation in rice with plasmids bearing a donor cassette released in planta gave higher gene knock-in efficiency with additional free dsDNA donor fragments (36% of lines regenerated on selective media) than without (25% of lines regenerated on selective media) [67]. To strongly increase their copy number in the transformed cells, the sequences coding for the CRISPR-Cas9 system and the donor template can also be inserted into disarmed viral replicons of geminiviruses [8,27,89,90]. Other types of viral vectors, such as Tobacco Mosaic Virus or Tobacco Rattle Virus, have been long used for transient expression of recombinant proteins in plants [91], but these viral constructs are too low cargo for the delivery of the CRISPR coding elements all together.…”

Section: Copy Numbermentioning

confidence: 99%

Towards mastering CRISPR-induced gene knock-in in plants: Survey of key features and focus on the model Physcomitrella patens

Collonnier

Guyon‐Debast

Maclot

et al. 2017

Methods

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a b s t r a c tBeyond its predominant role in human and animal therapy, the CRISPR-Cas9 system has also become an essential tool for plant research and plant breeding. Agronomic applications rely on the mastery of gene inactivation and gene modification. However, if the knock-out of genes by non-homologous end-joining (NHEJ)-mediated repair of the targeted double-strand breaks (DSBs) induced by the CRISPR-Cas9 system is rather well mastered, the knock-in of genes by homology-driven repair or end-joining remains difficult to perform efficiently in higher plants. In this review, we describe the different approaches that can be tested to improve the efficiency of CRISPR-induced gene modification in plants, which include the use of optimal transformation and regeneration protocols, the design of appropriate guide RNAs and donor templates and the choice of nucleases and means of delivery. We also present what can be done to orient DNA repair pathways in the target cells, and we show how the moss Physcomitrella patens can be used as a model plant to better understand what DNA repair mechanisms are involved, and how this knowledge could eventually be used to define more performant strategies of CRISPR-induced gene knock-in.

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Section: Copy Numbermentioning

confidence: 99%

Towards mastering CRISPR-induced gene knock-in in plants: Survey of key features and focus on the model Physcomitrella patens

Collonnier

Guyon‐Debast

Maclot

et al. 2017

Methods

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“…[221,222]. Several examples were cited in Section 2 of the successful application of CRISPR/Cas9 technologies in developing crop disease resistance [69,70,[106][107][108][109], and many others are expected. Indeed, CRISPR/Cas9 technologies may facilitate the development of entirely novel GE strategies not presented in this review.…”

Section: Genome Editing: More Precise Dynamic Tools For Gementioning

confidence: 99%

“…Plants can be transformed to produce both Cas9 and a target-specific gRNA, in order to cleave a specified target of invading DNA. For example, a Cas9/gRNA complex can be engineered to target the replicating DNA of Geminiviruses, which are highly destructive to crops in tropical and subtropical climates [106][107][108][109]. Such an engineered Cas9/gRNA complex produces a sequence-specific, targeted immune response which can result in significant host resistance against a DNA virus.…”

Section: Engineering Crispr/cas Immune Systemmentioning

confidence: 99%

Genetic Engineering and Sustainable Crop Disease Management: Opportunities for Case-by-Case Decision-Making

Vincelli

2016

Sustainability

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Genetic engineering (GE) offers an expanding array of strategies for enhancing disease resistance of crop plants in sustainable ways, including the potential for reduced pesticide usage. Certain GE applications involve transgenesis, in some cases creating a metabolic pathway novel to the GE crop. In other cases, only cisgenessis is employed. In yet other cases, engineered genetic changes can be so minimal as to be indistinguishable from natural mutations. Thus, GE crops vary substantially and should be evaluated for risks, benefits, and social considerations on a case-by-case basis. Deployment of GE traits should be with an eye towards long-term sustainability; several options are discussed. Selected risks and concerns of GE are also considered, along with genome editing, a technology that greatly expands the capacity of molecular biologists to make more precise and targeted genetic edits. While GE is merely a suite of tools to supplement other breeding techniques, if wisely used, certain GE tools and applications can contribute to sustainability goals.

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“…However, Agrobacterium mediated gene transformation is easy and commonly used in many experiments. Ali et al (2015), Baltes et al (2015), Ji et al (2015) explained the use of CRISPR/Cas9 technique for protection of plants against geminiviruses. Ali et al (2015) performed experiment to demonstrate the efficacy of CRISPR/Cas9 against tomato yellow leaf curl virus (TYLCV) in Nicotiana benthamiana plants and their results exhibited profound evidence of interference against viral DNA by use of guide RNA mediated through Agrobacterium tumefaciens.…”

Section: Use Of Crispr/cas9 On Disease Resistancementioning

confidence: 99%

Use of Crispr/Cas9 for Development of Disease Resistant Cultivars in Plant Breeding

Ghimire

2017

Int J Appl Sci Biotechnol

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Crop protection against pests and diseases is a major challenge in agriculture. Plant breeding is a key solution for the development of disease resistant cultivars. Gene editing is an indispensable part of plant breeding to obtain desirable traits in crops. CRISPR (Clustered Regular Interspaced Palindromic Repeats)/Cas9 (CRISPR-associated protein) is a recent breakthrough in gene editing technology. It can be utilized to exploit defensive mechanism in plants against pathogen attack with recognition and degradation of the invading pathogenic genes by bacterial immune system. Advances in plant breeding with integration of CRISPR/Cas9 have facilitated the production of cultivars with heritable resistance to viral and bacterial disease. CRISPR/Cas9 mediated genetically engineered resistance can be inherited to further generation of crops after segregation of Cas9/sgRNA transgene in F1 generation. The segregation of Cas9/sgRNA transgene prevents undesirable genome modification in successive generation and makes use of CRISPR/Cas9 safe in plant breeding. CRISPR/Cas9 proves itself as a fascinating tool to revolutionize plant breeding for the development of various disease resistant cultivars however, effects of CRISPR/Cas9 system on different physiological process of plants still need to be studied.

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Conferring resistance to geminiviruses with the CRISPR–Cas prokaryotic immune system

Cited by 374 publications

References 15 publications

Towards mastering CRISPR-induced gene knock-in in plants: Survey of key features and focus on the model Physcomitrella patens

Towards mastering CRISPR-induced gene knock-in in plants: Survey of key features and focus on the model Physcomitrella patens

Genetic Engineering and Sustainable Crop Disease Management: Opportunities for Case-by-Case Decision-Making

Use of Crispr/Cas9 for Development of Disease Resistant Cultivars in Plant Breeding

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