Confining Natural/Mimetic Enzyme Cascade in an Amorphous Metal–Organic Framework for the Construction of Recyclable Biomaterials with Catalytic Activity

Ji, Yuan; Gao, Wanning; Zhang, Shilin; Li, Bingzhi; Huang, He; Zhang, Xing

doi:10.1021/acs.langmuir.1c02093

Cited by 28 publications

(23 citation statements)

References 54 publications

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“…Because of the development of nanotechnology, biotechnology, and catalytic mechanism, researchers have obtained a deeper understanding of nanozymes. In addition to the magnetic Fe 3 O 4 nanoparticles, other kinds of materials also possess enzyme-like catalytic properties, including but not limited to iron-based materials, , precious-metal materials, , carbon-based materials, , metal sulfides, , metal–organic frameworks, and so on. , At present, the enzyme-like properties of nanozymes have expanded from peroxidase-like properties , to catalase-like, − oxidase, , superoxide dismutase, − lactic acid enzyme, and other different properties.…”

Section: Introductionmentioning

confidence: 99%

Mechanism and Application of Surface-Charged Ferrite Nanozyme-Based Biosensor toward Colorimetric Detection of l-Cysteine

Liu

Chen

et al. 2022

Langmuir

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Peroxidase-like nanozymes with robust catalytic capacity and detection specificity have been proposed as substitutes to natural peroxidases in biochemical sensing. However, the catalytic activity enhancement, detection mechanism, and application of nanozyme-based biosensors toward L-cysteine (L-Cys) detection still remain significant challenges. In this work, a doped ferrite nanozyme with well-defined structure and surface charges is fabricated by a two-step method of continuous flow coprecipitation and high-temperature annealing. The resulted ferrite nanozyme possesses an average size of 54.5 nm and a zeta-potential of 6.45 mV. A high-performance biosensor is manufactured based on the peroxidase-like catalytic feature of the doped ferrite. The ferrite nanozyme can oxidize the 3,3′,5,5′tetramethylbenzidine (TMB) with the assistance of H 2 O 2 because of the instinctive capacity to decompose H 2 O 2 into •OH. The Michaelis−Menten constants (0.0911 mM for TMB, 0.140 mM for H 2 O 2 ) of the ferrite nanozyme are significantly smaller than those of horseradish peroxidase. A reliable colorimetric method is established to selectively analyze L-Cys via a facile mixing-anddetecting methodology. The detection limit and linear range are 0.119 μM and 0.2−20 μM, respectively. Taking the merits of the ferrite nanozyme-based biosensors, the L-Cys level in the human serum can be qualitatively detected. It can be anticipated that the surface-charged ferrite nanozyme shows great application prospects in the fields of bioanalytical chemistry and point-of-care testing.

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Section: Introductionmentioning

confidence: 99%

Mechanism and Application of Surface-Charged Ferrite Nanozyme-Based Biosensor toward Colorimetric Detection of l-Cysteine

Liu

Chen

et al. 2022

Langmuir

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“…As shown in Figure 9B , LDH/MNPs@MAF-7 demonstrated better tolerance under acidic conditions, and the activity reached the maximum at pH 6. This indicates that the three-dimensional structure of MAF-7 provided a microenvironment, thus helping the LDH retain its activity and preventing the subunits dissociation ( Li et al, 2018 ; Ji et al, 2022 ).…”

Section: Resultsmentioning

confidence: 97%

“…The enhanced thermal stability could be illustrated that LDH was protected by the rigid structure of MAF-7. This can minimize the unfolding of the enzyme ( Ji et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

One-pot encapsulation of lactate dehydrogenase and Fe3O4 nanoparticles into a metal–organic framework: A novel magnetic recyclable biocatalyst for the synthesis of D-phenyllactic acid

Sun

Wang

et al. 2023

Front. Bioeng. Biotechnol.

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The main challenges in bio-catalysis of d-phenyllactic acid (D-PLA) are poor tolerance of lactate dehydrogenase (LDH) to harsh environmental conditions and inability to recycle the catalyst. A novel magnetic framework composite was prepared as solid support for the immobilization of enzymes via one-pot encapsulation in this study. LDH/MNPs@MAF-7 was synthesized by the one-pot encapsulation of both LDH and magnetic nanoparticles (MNPs) in MAF-7. The LDH/MNPs@MAF-7 showed stable biological activity for the efficient biosynthesis of D-PLA. The structure and morphology of LDH/MNPs@MAF-7 were systematically characterized by SEM, FT-IR, XRD, VSM, XPS, TGA and N2 sorption. These indicated that LDH/MNPs@MAF-7 was successfully synthesized, exhibiting enhanced resistance to acid and alkali, temperature and organic solvents. Furthermore, the bio-catalyst could be separated easily using a magnet, and the reusability was once considerably expanded with 80% of enzyme activity last after eight rounds of recycling. Therefore, LDH/MNPs@MAF-7 could be used as a potential biocatalyst for the biosynthesis of D-PLA due to its good stability and recovery properties.

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“…Among various POD-mimicking enzymes, MOFs constructed by transition metal ions and organic ligands have shown robust vitality in the field of catalysis owing to their versatile synthetic conditions, high crystallinity, large surface area, rich active sites, and tunable structures . Given the advantages of MOF materials in structure and properties, the POD-mimicking behaviors of MOFs have been proposed. − Zheng et al synthesized MOF-808 by the solvothermal method for glucose detection . Zhao et al synthesized Fe-BTC by a one-pot method for glucose detection .…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Colorimetric Detection of Glutathione in Human Serum Based on Iron–Copper Metal–Organic Frameworks

Chen

et al. 2022

Langmuir

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Emerging metal–organic framework (MOF)-based mimic enzymes have been exploited to design a colorimetric sensor for the detection of biomolecules. However, it is challenging to figure out the glutathione (GSH) detection method and the corresponding sensing mechanism using an MOF-based colorimetric sensor. In this work, a novel iron–copper MOF with high activity is synthesized by a wet-chemical method. A GSH colorimetric sensor based on the peroxidase-like properties of the iron–copper MOF is developed. Hydrogen peroxide is converted to hydroxyl radicals by the peroxidase-like properties of the iron–copper MOF mimic enzyme, which can catalyze the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxidized TMB (ox-TMB). The kinetic constant of the MOF mimic enzyme (0.02 mM for H2O2) is superior to horseradish peroxidase (HRP). The GSH content can be quantified by proposing a sensor based on the colorimetric method and color turn-off mechanism. The turn-off mechanism of GSH analysis includes two aspects. On the one hand, the blue ox-TMB can be deoxidized to colorless TMB by GSH. On the other hand, hydroxyl radicals (•OH) can be consumed by GSH. The linear range and limit of detection are 2–20 and 0.439 μM, respectively. At the same time, GSH detection also shows good specificity and anti-interference characteristics. Therefore, MOF-based colorimetric sensors have been used to qualitatively and quantitatively measure GSH contents in human serum. The mechanism and application of the iron–copper MOF pave a way for the development of mimic enzymes with polymetallic active sites in the field of colorimetric sensing.

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Confining Natural/Mimetic Enzyme Cascade in an Amorphous Metal–Organic Framework for the Construction of Recyclable Biomaterials with Catalytic Activity

Cited by 28 publications

References 54 publications

Mechanism and Application of Surface-Charged Ferrite Nanozyme-Based Biosensor toward Colorimetric Detection of l-Cysteine

Mechanism and Application of Surface-Charged Ferrite Nanozyme-Based Biosensor toward Colorimetric Detection of l-Cysteine

One-pot encapsulation of lactate dehydrogenase and Fe3O4 nanoparticles into a metal–organic framework: A novel magnetic recyclable biocatalyst for the synthesis of D-phenyllactic acid

Highly Sensitive Colorimetric Detection of Glutathione in Human Serum Based on Iron–Copper Metal–Organic Frameworks

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