Confirmation of Eprinomectin, Moxidectin, Abamectin, Doramectin, and Ivermectin in Beef Liver by Liquid Chromatography/Positive Ion Atmospheric Pressure Chemical Ionization Mass Spectrometry

Ali, M. Sher; Sun, Tung; Mcleroy, G. E.; Phillippo, E. T.

doi:10.1093/jaoac/83.1.39

Cited by 35 publications

(22 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Th erefore, the avermectin data are inconclusive with regard to whether hydrophobic partitioning or more specifi c interactions are involved in sorption of avermectin. From LC-MS data, it is known that ivermectin is capable of forming adducts with cations, such as ammonium or sodium (Ali et al, 2000). Hence, according to Oppel et al (2004), there are reasons to presume that ivermectin binds to soil by the formation of adducts or complexes with immobile, inorganic soil matter.…”

Section: Solid-water Distribution Coeffi Cient Versus Organic Carbon-mentioning

confidence: 99%

“…Despite sorption via hydrophobic binding, other sorption mechanisms have been suggested, for example, specifi c binding of ivermectin to soil via formation of adducts or complexes with immobile, inorganic soil matter (Oppel et al, 2004). Th ese suggestions are further supported by liquid chromatography-mass spectrometry (LC-MS) data, where ivermectin was capable of forming adducts with cations such as ammonium or sodium (Ali et al, 2000) Th us, there are reasons to presume that ivermectin binds to soil by the formation of adducts or complexes with immobile, inorganic soil matter.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Sorption and Mobility of Ivermectin in Different Soils

et al. 2008

View full text Add to dashboard Cite

Avermectins are widely used to treat livestock for parasite infections. Ivermectin, which belongs to the group of avermectins, is particularly hazardous to the environment, especially to crustaceans and to soil-dwelling organisms. Sorption is one of the key factors controlling transport and bioavailability. Therefore, batch studies have been conducted to characterize the sorption and desorption behavior of ivermectin in three European soils (Madrid, York, and artificial soil). The solid-water distribution coefficient (K(d)) for ivermectin sorption to the tested soils were between 57 and 396 L kg(-1) (determined at 0.1 microg g(-1)), while the organic carbon-normalized sorption coefficients (K(oc)) ranged from 4.00 x 10(3) to 2.58 x 10(4) L kg(-1). The Freundlich sorption coefficient (K(F)) was 396 (after 48 h) for the artificial soil over a concentration range of 0.1 to 50 microg g(-1), with regression constants indicating a concentration-dependent sorption. The obtained data and data in the literature are inconclusive with regard to whether hydrophobic partitioning or more specific interactions are involved in sorption of avermectins. For abamectin, hydrophobic partitioning seems to be one of the dominant types of binding, while hydrophobicity is less important for ivermectin, which is probably due to the lower lipophilicity of the molecule. Furthermore, the presence of cations such as Ca(2+) leads to decreasing sorption. Thus, it is presumed that ivermectin binds to soil by formation of complexes with immobile, inorganic soil matter. In contrast to abamectin, hysteresis could be excluded for ivermectin in the studied soils for the evaluation of sorption and desorption. The sorption mechanism is highly dependent on physicochemical properties of the avermectin.

show abstract

Section: Solid-water Distribution Coeffi Cient Versus Organic Carbon-mentioning

confidence: 99%

mentioning

confidence: 99%

Sorption and Mobility of Ivermectin in Different Soils

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Recently, a liquid chromatographytandem mass spectrometry and fluorescence detection method was used to determine avermectin and milbemycin residues in bovine muscle [19]. Liver tissues are regarded as the matrix of choice for the determination of residues but are particularly difficult to work with, due to the complex matrix interference that may be present [20]. Histological changes provide a rapid means for detecting the effects of pesticides in various animal tissues and organs.…”

Section: Introductionmentioning

confidence: 99%

Effect of sub-acute exposure to abamectin “insecticide” on liver rats (Rattus norvegicus)

Khaldoun-Oularbi¹,

Richeval²,

Djenas³

et al. 2013

Ann Toxicol Anal

View full text Add to dashboard Cite

show abstract

“…2,18,36 These methods were used to confirm presence of moxidectin in bovine fat at 250 ppb 36 and multiresidue detection of 4 avermectins and moxidectin at 25 ppb in liver. 1 This article proposes a simple, rapid, and sensitive multiresidue method for isolation and detection of moxidectin, doramectin, selamectin, ivermectin, and eprinomectin by LC/MS with positive atmospheric pressure chemical ionization (APCI) set to run in selected ion monitoring (SIM) mode to increase sensitivity of detection. Purification, isolation, and detection are described for all 5 macrolide endectocides at a low ppb levels with no requirements for sample derivatization.…”

mentioning

confidence: 99%

Isolation and Multiresidue Detection of Macrolide Endectocides Present in Animal Matrices

2002

View full text Add to dashboard Cite

A simple, rapid, and sensitive method for isolation and detection of macrolide endectocides (moxidectin, doramectin, selamectin, ivermectin, and eprinomectin) in animal sera and liver is described. Fortified sera or homogenized liver samples were treated with sodium chloride followed by organic solvent extraction. No additional steps were required prior to analysis. Separation of analytes and collection of mass information was achieved by liquid chromatography/mass spectrometry with positive atmospheric pressure chemical ionization set in selected ion monitoring mode with each sample analysis complete in 15 minutes. Presence of each compound was confirmed based on 2 separate extracted ion profiles. Detection of avermectins and moxidectin in a range of working standards was achieved at 10, 50, and 100 ppb. Quantitation of these compounds in fortified samples was based on standard calibration curves with R2 > 0.99. Detection limits of 10 ppb for ivermectin, moxidectin, and doramectin, 50 ppb for selamectin, and 100 ppb for eprinomectin were achieved in spiked sera. Recoveries of avermectins and moxidectin in 500 ppb fortified sera fell between 61 and 89% (+/-5.7-15.7). Analysis of fortified liver gave comparable results with recovery of selamectin of 83-91% +/- 18.3. A complete mass spectral fragmentation pattern of selamectin and affordable screening method for 6 macrolide endectocides are reported. Method comparison for salt treatment and solid-phase extraction of fortified samples is discussed.

show abstract

Confirmation of Eprinomectin, Moxidectin, Abamectin, Doramectin, and Ivermectin in Beef Liver by Liquid Chromatography/Positive Ion Atmospheric Pressure Chemical Ionization Mass Spectrometry

Cited by 35 publications

References 1 publication

Sorption and Mobility of Ivermectin in Different Soils

Sorption and Mobility of Ivermectin in Different Soils

Effect of sub-acute exposure to abamectin “insecticide” on liver rats (Rattus norvegicus)

Isolation and Multiresidue Detection of Macrolide Endectocides Present in Animal Matrices

Contact Info

Product

Resources

About