Confirmation of hybridity using GISH and determination of 18S rDNA copy number using FISH in interspecific F<sub>1</sub>hybrids of<i>Tecoma</i>(Bignoniaceae)

Contreras, Ryan N.; Ruter, John M.; Conner, Joann A.; Zeng, Yajuan; Ozias‐Akins, Peggy

doi:10.1139/g2012-030

Cited by 4 publications

(2 citation statements)

References 24 publications

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“…Besides confirming the hybrid character, GISH allowed the observation of recombinant chromosomes in backcrossed hybrids (BC 1 ). The use of GISH to identify natural hybrids, to confirm artificial hybridization (Marasek et al, 2004;Contreras et al, 2012), and to identify recombined chromosomes in backcrossed progenies BC 1 and BC 2 (Karlov et al, 1999;Lim et al, 2000b;Marasek-Ciolakowska et al, 2012) has been widely reported for different plant groups. Hybrids BC 1 HD18-106 and HD18-113 showed different numbers of recombinant chromosomes, denoting variation in the meiotic recombination amongst homeologous chromosomes in HD13 hybrids.…”

Section: Discussionmentioning

confidence: 99%

Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora

Melo¹,

Silva²,

Souza³

2015

Genet. Mol. Res.

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ABSTRACT. The genomic in situ hybridization (GISH) technique was applied to Passiflora interspecific F 1 HD13-133 hybrids (Passiflora sublanceolata x Passiflora foetida) and HD15-101 (Passiflora gardineri x Passiflora gibertii), and the backcrossed hybrids (BC 1 ) HD18-106 and HD18-113 (Passiflora sublanceolata x HD13-133). GISH was performed using genomic probes prepared with the DNA from the paternal genitor, whereas the maternal DNA was used as blocking DNA and employed at various concentrations (20X, 40X, 60X, and 100X) in relation to the probe concentration. At the same time, GISH was applied with the use of simultaneous probes from both genomes, paternal and maternal, that were detected with avidin-FITC and antidigoxigenin-rhodamine, respectively. Both methodologies allowed the distinguishing of the maternal and paternal genomes, thus confirming the hybrid nature of all the analyzed genotypes. Furthermore, the presence of recombinant chromosomes in BC 1 hybrids revealed the occurrence of meiotic recombination in HD13 hybrids. This application 2177 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (1): 2176-2188 (2015 GISH technique for Passiflora of the GISH technique is an important step towards genomic analyses of Passiflora hybrids: it can broaden the phylogenetic and evolutionary studies of the genus and, at the same time, contribute to breeding programs.

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Section: Discussionmentioning

confidence: 99%

Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora

Melo¹,

Silva²,

Souza³

2015

Genet. Mol. Res.

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“…Taxonomic examination of Tecoma genus is regarded as a cumbersome task due to the existence of poorly demarcated species and hybrids that possess complex overlapping vegetative characters [5]. Besides, the African species are treated as members of Tecomaria, due to the lack of supporting morphological, phytochemical or molecular evidence [6].…”

Section: Introductionmentioning

confidence: 99%

Biodiversity Assessment in Tecoma Species Using Micro-morphometric, Phytochemical and Molecular Descriptors Aided by Multivariate Analysis

et al. 2022

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Abstract. Tecoma genus is a medicinally-valuable member in Bignoniaceae family comprising fourteen defectively-demarcated species with overlapping vegetative characters. Our study aimed to explore the micro-morphometric, phytochemical and genetic diversity among eight cultivated Tecoma plants. The variabilities existing in three data matrices derived from micro-morphometric features of powdered leaves, high performance liquid chromatographic fingerprints and Random Amplified Polymorphic DNA (RAPD) fingerprints were explored by multivariate analysis. The microscopical measurements of non-glandular trichomes, i.e. collapsed multicellular and branched, and glandular trichomes were revealed to be the significant botanical discriminatory features of the powdered plant specimens, however species-specific phytochemical markers were identified to be gallic acid, catechin and chlorogenic acid for T. radicans, T. grandiflora, T. capensis var. yellow, respectively. Sinapic acid was enriched in T capensis and its varieties i.e. pink, red and harmony. RAPD primers i.e. OP-B02, OP-C04 and OP-K2 served as molecular descriptors producing the highest polymorphism among the plants. The variabilities within two trumpet creeper plants “T. radicans and T. grandiflora” were unveiled via their micro-morphometric features as well as genetic fingerprints. Tecoma hybrid “Tecoma ×smithii” was more micro-morphometrically similar to the Chinese “T. grandiflora.” than T. capensis which is one of its parent plants, and proved to be more genetically related to it. This is the report for biodiversity in botanical, chemical and genetic aspects of Tecoma plants which are essential for the comprehensive authentication and differentiation among the studied plants assisted by multivariate analysis. The presented approach provides a competent and time-saving tool for simultaneously-discriminating among closely-related species. Resumen. El género Tecoma es un miembro medicinalmente valioso de la familia Bignoniaceae que comprende catorce especies demarcadas defectuosamente con caracteres vegetativos superpuestos. Nuestro estudio tuvo como objetivo explorar la diversidad micromorfométrica, fitoquímica y genética entre ocho plantas de Tecoma cultivadas. Se exploraron mediante análisis multivariado las variabilidades existentes en tres matrices de datos derivadas de las características micromorfométricas de las hojas en polvo, las huellas dactilares por medio de cromatografía líquida de alta resolución y las huellas dactilares de ADN polimórfico amplificado al azar (RAPD). Las mediciones microscópicas de tricomas no glandulares, esto es, tricomas multicelulares colapsados y ramificados, y tricomas glandulares mostraron ser las características significativas de discriminación botánica de las muestras en polvo de plantas. Se identificaron marcadores fitoquímicos específicos para algunas especies como ácido gálico, catequina y ácido clorogénico para T. radicans, T. grandiflora, T. capensis var. amarillo, respectivamente. El ácido sinápico está enriqueció en T capensis y sus variedades, esto es, rosa, rojo y armonía. Los cebadores RAPD, esto es, OP-B02, OP-C04 y OP-K2 sirvieron como descriptores moleculares que producen el polimorfismo más elevado entre las plantas. A través de sus características micromorfométricas y sus huellas genéticas se determinó la variabilidad en dos plantas trepadoras de trompeta “T. radicans y T. grandiflora.” El híbrido de Tecoma “Tecoma × smithii” es micromorfométricamente más similar al chino “T. grandiflora” que a T. capensis, que es una de sus plantas progenitoras, y mostró estar más relacionada genéticamente con ella. En esta investigación de biodiversidad en aspectos botánicos, químicos y genéticos de las plantas de Tecoma que son esenciales para la autenticación y diferenciación integral entre las plantas estudiadas con la ayuda del análisis multivariado. El enfoque presentado proporciona una herramienta eficiente y que ahorra tiempo para discriminar simultáneamente entre especies estrechamente relacionadas.

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FISH and GISH: molecular cytogenetic tools and their applications in ornamental plants

et al. 2015

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The innovations in chromosome engineering have improved the efficiency of interrogation breeding, and the identification and transfer of resistance genes from alien to native species. Recent advances in molecular biology and cytogenetics have brought revolutionary, conceptual developments in mitosis and meiosis research, chromosome structure and manipulation, gene expression and regulation, and gene silencing. Cytogenetic studies offer integrative tools for imaging, genetics, epigenetics, and cytological information that can be employed to enhance chromosome and molecular genomic research in plant taxa. In situ hybridization techniques, such as fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH), can identify chromosome morphologies and sequences, amount and distribution of various types of chromatin in chromosomes, and genome organization during the metaphase stage of meiosis. Over the past few decades, various new molecular cytogenetic applications have been developed. The FISH and GISH techniques present an authentic model for analyzing the individual chromosome, chromosomal segments, or the genomes of natural and artificial hybrid plants. These have become the most reliable techniques for studying allopolyploids, because most cultivated plants have been developed through hybridization or polyploidization. Moreover, introgression of the genes and chromatin from the wild types into cultivated species can also be analyzed. Since hybrid derivatives may have variable alien chromosome numbers or chromosome arms, the use of these approaches opens new avenues for accurately identifying genome differences.

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Confirmation of hybridity using GISH and determination of 18S rDNA copy number using FISH in interspecific F₁hybrids ofTecoma(Bignoniaceae)

Cited by 4 publications

References 24 publications

Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora

Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora

Biodiversity Assessment in Tecoma Species Using Micro-morphometric, Phytochemical and Molecular Descriptors Aided by Multivariate Analysis

FISH and GISH: molecular cytogenetic tools and their applications in ornamental plants

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Confirmation of hybridity using GISH and determination of 18S rDNA copy number using FISH in interspecific F1hybrids ofTecoma(Bignoniaceae)

Cited by 4 publications

References 24 publications

Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora

Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora

Biodiversity Assessment in Tecoma Species Using Micro-morphometric, Phytochemical and Molecular Descriptors Aided by Multivariate Analysis

FISH and GISH: molecular cytogenetic tools and their applications in ornamental plants

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Confirmation of hybridity using GISH and determination of 18S rDNA copy number using FISH in interspecific F₁hybrids ofTecoma(Bignoniaceae)