Confirmation of the constitution of alfalfa mosaic virus hybrid genomes by backcross experiments

Dingjan-Versteegh, Annemieke; Vloten-Doting, Lous Van; Jaspars, E.M.J.

doi:10.1016/0042-6822(74)90231-1

Cited by 8 publications

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“…However, in our hands single lesion transfer proved to be a reliable method for the separation of AMV from other viruses, e.g. from tobacco necrosis virus; or tobacco streak virus, cucumber mosaic virus (unpublished results) for the separation of different hybrids of AMV as well as for the separation of mutants from wild type (7,8). Furthermore there is no reason to assume that single lesion transfer purification of artificially induced mutants would be more efficient than that of mutants selected by host shift.…”

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confidence: 92%

Plant-virus-based vectors for gene transfer will be of limited use because of the high error frequency during viral RNA synthesis

1985

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The error frequency during the RNA replication of alfalfa mosaic virus (AMV) was calculated to be significantly higher than 10(-5). It may be expected that RNA synthesis in general will have low fidelity compared to DNA synthesis. The low fidelity of RNA replication will severely restrict the usefulness of vectors for genetic engineering which are based on RNA viruses, viroids or DNA viruses which are replicated via an RNA intermediate (e.g. caulimoviruses). Spontaneous mutants selected by host shift were found to be much less stable than UV-induced mutants. This difference points to variations in fidelity during RNA synthesis, probably due to the local sequence of the template.

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confidence: 92%