Confirmatory method for macrolide residues in bovine tissues by micro-liquid chromatography–tandem mass spectrometry

Determination of Erythromycin, Spiramycin, Tilmicosin and Tylosin in Animal Feedingstuffs by Liquid Chromatography-Tandem Mass Spectrometry

“…The method was validated according to the criteria laid down in the European Commission Decision 2002/657/ EC for confirmatory purposes in LC technique using ROX as internal standard [3,13] . The validation included the determination of selectivity/specificity, linearity, recovery, repeatability, reproductibility, the decision limit (CCα), detection capability (CCβ), the limits of detection (LOD) and quantification (LOQ).…”

“… İletişim (Correspondence)  +90 224 2941338  artunyibar@uludag.edu.tr Determination of Erythromycin ... mycoplasms, and members of the chlamidia group [2,3] . The macrolides reversibly bind to the 50S ribosomal unit and inhibit bacterial protein biosynthesis [4] .…”

“…They are widely used in veterinary medicine and in the husbandry of livestock to prevent and treat respiratory diseases, necrotic enteritis and to promote growth in foodproducing animals when incorporated at subtherapeutic level into feedingstuffs or drinking water. Antibiotics in animal feedingstuff, in general, are used regularly to induce growth promotion and feed conversion ratio and to increase weight gain than to combat specific diseases [3,5] . Overuse and incorrect use of these antibiotics in animal feedingstuffs can cause harmful residue problems in the human food chain [5,6] .…”

“…A number of methods for the detection of macrolides in various of animal tissues have been proposed [1,3,6,11,12] . The aim of this study was to determine the feasibility, sensitivity, rapidity, simplicity and suitability of LC-MS/MS for the direct detection and quantitation of erythromycin (ERY), spiramycin (SPI), tilmicosin (TIL), and tylosin (TYL) in animal feedingstuffs.…”

Aspectos analíticos e regulatórios na determinação de resíduos de macrolídeos em alimentos de origem animal por cromatografia líquida associada à espectrometria de massas

“…A presença de resíduos dessas substâncias em produtos alimentícios é uma questão de grande interesse dos órgãos governamentais responsáveis pela saúde pública e das indústrias de alimentos, pois representa riscos à saúde dos consumidores, ressaltando-se a seleção de formas resistentes de microrganismos, a carcinogenicidade, mutagenicidade, teratogenicidade e a hipersensibilidade. [1][2][3][4][5][6] Sendo o Brasil um país de expressiva atividade pecuária no cenário mundial, é fundamental que estejam em vigor programas eficientes que garantam a qualidade de alimentos desta origem. Para atender a essa demanda, é necessário o desenvolvimento de métodos analíticos confiáveis que possibilitem a determinação de medicamentos veterinários em níveis residuais (na ordem de ng g -1 ), haja vista que são substâncias potencialmente tóxicas para a saúde humana e para o meio ambiente.…”