Confocal immunohistochemistry of the dermal glands and evolutionary considerations in the caecilian, <i>Typhlonectes natans</i> (Amphibia: Gymnophiona)

Lauriano, Eugenia Rita; Żuwała, Krystyna; Kuciel, Michał; Budzik, Karolina A.; Capillo, Gioele; Alesci, Alessio; Pergolizzi, Simona; Dugo, Giacomo; Zaccone, Giacomo

doi:10.1111/azo.12112

Cited by 26 publications

(21 citation statements)

References 39 publications

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“…The cutaneous glands of Ichthyophis orthoplicatus and Ichthyophis kohtaoensis , which likely secrete bioactive molecules, have been reported by Fox (). Lauriano et al () investigated the immunohistochemistry and the evolutionary aspects of the cutaneous glands of Typhlonectes natans , a South American caecilian species. Arun et al () reported the ultrastructural organization of the cutaneous glands of Ichthyophis tricolor and Uraeotyphlus cf.…”

Section: Introductionmentioning

confidence: 99%

Light and transmission electron microscopic structure of skin glands and dermal scales of a caecilian amphibian Gegeneophis ramaswamii, with a note on antimicrobial property of skin gland secretion

Arun

Akbarsha

Oommen

et al. 2019

Microscopy Res & Technique

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Amphibian skin secretions contain a variety of bioactive compounds that are involved in diverse roles such as communication, homeostasis, defence against predators, pathogens, and so on. Especially, the caecilian amphibians possess numerous cutaneous glands that produce the secretory material, which facilitate survival in their harsh subterranean environment. Inspite of the fact that India has a fairly abundant distribution of caecilian amphibians, there has hardly been any study on their skin and its secretion. Herein, we describe, using light microscopy and electron microscopy, two types of dermal glands, mucous and granular, in Gegeneophis ramaswamii. The mucous glands are filled with mucous materials. The mucous‐producing cells are located near the periphery. The granular glands are surrounded by myoepithelial cells. A large number of granules of different sizes are present in the lumen of the granular gland. The granule‐producing cells are present near the myoepithelial lining of the gland. There are small flat disk‐like dermal scales in pockets in the transverse ridges of the posterior region of the body. Each pocket contains 1–4 scales of various sizes. Scanning electron microscopic (SEM) study of the skin surface showed numerous funnel‐shaped glandular openings. The antibacterial activity of the skin secretions was revealed in the test against Escherichia coli, Klebsiella pneumoniae, and Aeromonas hydrophila, all gram‐negative bacteria. SEM analyses confirm the membrane damage in bacterial cells on exposure to skin secretions of G. ramaswamii.

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Section: Introductionmentioning

confidence: 99%

Light and transmission electron microscopic structure of skin glands and dermal scales of a caecilian amphibian Gegeneophis ramaswamii, with a note on antimicrobial property of skin gland secretion

Arun

Akbarsha

Oommen

et al. 2019

Microscopy Res & Technique

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“…Kidney and gill samples were fixed in 4% paraformaldehyde in 0.1 M phosphate-buffered saline (pH 7.4) for 12–18 h, then dehydrated in graded ethanol, cleared in xylene, and embedded in Paraplast ® (McCormick Scientific LLC, St. Louis, MO, USA). Finally, serial slices (3–5 m thick) were created using a rotary microtome (LEICA 2065 Supercut, Poway, CA, USA) [ 49 , 50 , 51 , 52 ].…”

Section: Methodsmentioning

confidence: 99%

Histological and Chemical Analysis of Heavy Metals in Kidney and Gills of Boops boops: Melanomacrophages Centers and Rodlet Cells as Environmental Biomarkers

Alesci

Cicero

Fumia

et al. 2022

Toxics

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Industrialization has resulted in a massive increase in garbage output, which is frequently discharged or stored in waterways like rivers and seas. Due to their toxicity, durability, bioaccumulation, and biomagnification, heavy metals (such as mercury, cadmium, and lead) have been identified as strong biological poisons. Their presence in the aquatic environment has the potential to affect water quality parameters and aquatic life in general. Teleosts’ histopathology provides a sensitive indicator of pollutant-induced stress, because their organs have a central role in the transformation of different active chemical compounds in the aquatic environment. In particular, the gills, kidneys, and liver are placed at the center of toxicological studies. The purpose of this study is to examine the morphological changes caused by heavy metals in the kidney and gills of Boops boops, with a focus on melanomacrophages centers (MMCs) and rodlet cells (RCs) as environmental biomarkers, using histological and histochemical stainings (hematoxylin/eosin, Van Gieson trichrome, Periodic Acid Schiff reaction, and Alcian Blue/PAS 2.5), and immunoperoxidase methods. Our findings show an increase of MMCs and RCs linked to higher exposure to heavy metals, confirming the role of these aggregates and cells as reliable biomarkers of potential aquatic environmental changes reflected in fish fauna. The cytological study of RCs and MMCs could be important in gaining a better understanding of the complicated immune systems of teleosts.

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“…Samples were fixed in 4% paraformaldehyde in phosphate‐buffered saline (PBS) 0.1 M (pH 7.4) for 12–18 h, dehydrated in graded ethanol, cleared in xylene, embedded in Paraplast® (McCormick Scientific LLC, St. Louis, MO). Finally, serial sections (3–5 μm thick) were obtained by a rotary microtome (LEICA 2065 Supercut) (Alesci et al, 2014; Icardo et al, 2015; Lauriano, Żuwała, et al, 2016; Zaccone et al, 2015; Zaccone, Lauriano, et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

Localization of vasoactive intestinal peptide and toll‐like receptor 2 immunoreactive cells in endostyle of urochordate Styela plicata (Lesueur, 1823)

Alesci

Pergolizzi

Cascio

et al. 2022

Microscopy Res & Technique

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The endostyle is the first component of the ascidian digestive tract, it is shaped like a through and is located in the pharynx's ventral wall. This organ is divided longitudinally into nine zones that are parallel to each other. Each zone's cells are physically and functionally distinct. Support elements are found in zones 1, 3, and 5, while mucoproteins secreting elements related to the filtering function are found in zones 2, 4, and 6. Zones 7, 8, and 9, which are located in the lateral dorsal section of the endostyle, include cells with high iodine and peroxidase concentrations. Immunohistochemical technique using the following antibodies, Toll-like receptor 2 (TLR-2) and vasoactive intestinal peptide (VIP), and lectin histochemistry (WGA-wheatgermagglutinin), were used in this investigation to define immune cells in the endostyle of Styela plicata (Lesueur, 1823). Our results demonstrate the presence of immune cells in the endostyle of S. plicata, highlighting that innate immune mechanisms are highly conserved in the phylogeny of the chordates. Research highlights• Immune cells positive to TLR-2 and VIP in the endostyle of Styela plicata.• Expression of WGA in several zones of endostyle.• Use of comparative biology to improve the knowledge about immunology in ascidians.

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Confocal immunohistochemistry of the dermal glands and evolutionary considerations in the caecilian, Typhlonectes natans (Amphibia: Gymnophiona)

Cited by 26 publications

References 39 publications

Light and transmission electron microscopic structure of skin glands and dermal scales of a caecilian amphibian Gegeneophis ramaswamii, with a note on antimicrobial property of skin gland secretion

Light and transmission electron microscopic structure of skin glands and dermal scales of a caecilian amphibian Gegeneophis ramaswamii, with a note on antimicrobial property of skin gland secretion

Histological and Chemical Analysis of Heavy Metals in Kidney and Gills of Boops boops: Melanomacrophages Centers and Rodlet Cells as Environmental Biomarkers

Localization of vasoactive intestinal peptide and toll‐like receptor 2 immunoreactive cells in endostyle of urochordate Styela plicata (Lesueur, 1823)

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