Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel

Abstract

The detail measurements of velocity profiles of in vitro blood flow in microchannels is

Particle imaging techniques for volumetric three-component (3D3C) velocity measurements in microfluidics

“…The scanning time was decreased considerably by the Nipkow disk and a rotating disk with microlenses to focus the laser. Typical full-field scanning rates were 50 Hz (Lima et al 2006), 120 Hz (Park et al 2004), 200 Hz (Lima et al 2007) up to 4,800 Hz (Kinoshita et al 2007). Liebling et al (2006) used a slit instead of pinholes to record a whole line of the sample at once.…”

In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system

“…A detailed description of the confocal system can be found elsewhere (Lima et al 2006). In brief, the confocal system consisted of an inverted microscope (IX71; Olympus, Japan) combined with a confocal scanning unit (CSU22; Yokogawa, Japan), a diode-pumped solid-state (DPSS) laser (Laser Quantum, UK) with an excitation wavelength of 532 nm, and a high-speed camera (Phantom v7.1; Vision Research, USA).…”

“…Recently, we demonstrated the ability of the confocal micro-PIV to measure both pure water and diluted suspensions of blood cells in a glass square microchannel (Lima et al 2006); however, glass microchannels are not ideal to study the flow properties of blood at microscopic level mainly due to fabrication problems and physicochemicals factors (Maeda 1996). First, glass-to-glass sealing is difficult because direct bonding needs extremely smooth and flat surfaces, and indirect bonding fills the channels with adhesives, sols, and metals.…”

Visualization study of motion and deformation of red blood cells in a microchannel with straight, divergent and convergent sections

“…Kruchenok et al [9] observed the orientation of individual RBCs in interference fringes and found that RBC rouleaux undergo disaggregation under the action of interference laser fields. Lima et al [10] measured velocity distribution of RBC suspension flow in a microchannel with a 0.1 × 0.1 mm 2 cross-section by confocal micro-PIV. They indicated that microchannels with dimensions on the order of 100 μm still obey macroscale flow theory.…”