Confocal Raman microscopy as a diagnostic tool for investigation of living neuroblastoma tumour cells

Effects of Cantharidinate on apoptosis of human colorectal cancer UTC-116 cells were investigated by means of 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, H and E staining, flow cytometry, and Raman Spectra analysis. The results showed Cantharidinate to exert inhibitory action on proliferation of human colorectal cancer UTC-116 cells, inducing apoptosis, arresting cells in G1 phase, with decline of S and G2 phases. In addition, the results of Raman spectrum showed significant changes in the UTC-116 cells chemical structure with stretching after the application of Cantharidinate. Taken together, these results suggest that the treatment of human colorectal cancer with Cantharidinate may be associated with multiple molecular mechanisms for apoptosis. Furthermore, similar to fluorouracil, Cantharidinate should be considered as novel assistant drug for controlling the growth of human colorectal cancer UTC-116 cells.

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“…It is a non-invasive method, which does not require any sample processing (Scalfi-Happ et al, 2007).…”

Section: Uv-vis Absorption Spectroscopy and Raman Spectroscopymentioning

confidence: 99%

Apoptosis of Colorectal Cancer UTC116 Cells Induced by Cantharidinate

Liu

Gao²,

Xu³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…29 The substrate on which the cells are deposited, however, affects the intensity of some of these bands as evidenced in the lower panel of Figure 5, where the difference between the two spectra is reported. Out of the experimental error, differences appear both in the low and in high frequency region.…”

Section: Resultsmentioning

confidence: 99%

“…Our data show that, the intensity of peaks around 1125 cm -1 , 1310 cm -1 , and 1580 cm -1 is smaller in the sample grown on the PANI substrate than on the control as evidenced in the difference spectrum. These peaks can be assigned to proteins, lipids and the breathing of the Guanine and Adenine rings respectively 29 but at about the same frequencies, characteristic Raman bands of Cytochrome C can be also recognized. 31 Due to the resonant scattering of Cytochrome C with the incident 532 nm excitation, here used, the intensity of its Raman bands probably prevails on the others.…”

Section: Resultsmentioning

confidence: 99%

A multidisciplinary approach to study the functional properties of neuron-like cell models constituting a living bio-hybrid system: SH-SY5Y cells adhering to PANI substrate

et al. 2016

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ARTICLES YOU MAY BE INTERESTED INPolysaccarides-based gels and solid-state electronic devices with memresistive properties: Synergy between polyaniline electrochemistry and biology AIP Advances 6, 111302 (2016) A living bio-hybrid system has been successfully implemented. It is constituted by neuroblastic cells, the SH-SY5Y human neuroblastoma cells, adhering to a poly-anyline (PANI) a semiconductor polymer with memristive properties. By a multidisciplinary approach, the biocompatibility of the substrate has been analyzed and the functionality of the adhering cells has been investigated. We found that the PANI films can support the cell adhesion. Moreover, the SH-SY5Y cells were successfully differentiated into neuron-like cells for in vitro applications demonstrating that PANI can also promote cell differentiation. In order to deeply characterize the modifications of the bio-functionality induced by the cell-substrate interaction, the functional properties of the cells have been characterized by electrophysiology and Raman spectroscopy. Our results confirm that the PANI films do not strongly affect the general properties of the cells, ensuring their viability without toxic effects on their physiology. Ascribed to the adhesion process, however, a slight increase of the markers of the cell suffering has been evidenced by Raman spectroscopy and accordingly the electrophysiology shows a reduction at positive stimulations in the cells excitability. © 2016 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license

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“…This technique is available in many microscopes and spectroscopic apparatus and offers important advantages respect to wide-field microscopy, such as the possibility to control the depth of field (Ref 2), a reduction of the background signal coming from regions away from the focal plane and the possibility to collect signals from specific regions of thick samples allowing a 3D reconstruction ( . This technique is extremely popular due to the easiness with which high-quality images can be obtained from samples prepared for conventional fluorescence microscopy; it is widely used in cell biology to study fixed and living cells and tissues (Ref [6][7][8][9][10][11], or in inhomogeneous materials for morphology characterization (Ref [12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Nonlocal Effects in the Confocal μ-Raman Characterization of Inhomogeneous Polymer Coatings

Rodrı́guez¹,

Vargas²,

Estévez³

2010

J. of Materi Eng and Perform

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The confocal l-Raman technique was used to characterize the morphology of inhomogeneous anti-graffiti coatings; for these systems, the antiadherent molecules were segregated to the external (exposed) surface forming a layer whose thickness was determined. The confocal data from these inhomogeneous coatings contains nonlocal contributions because the light scattered from sources near the specific specimen under analysis (the focused region) could not be completely rejected by the spatial filter of the confocal device. These nonlocal contributions had important effects in the Raman spectra, modifying the bands height profiles of homogeneous and inhomogeneous materials allowing their identification. Taking into account these nonlocal effects, it was possible to interpret correctly the relative intensities of the Raman bands and characterize properly the inhomogeneous coatings.

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Confocal Raman microscopy as a diagnostic tool for investigation of living neuroblastoma tumour cells

Cited by 21 publications

References 8 publications

Apoptosis of Colorectal Cancer UTC116 Cells Induced by Cantharidinate

Apoptosis of Colorectal Cancer UTC116 Cells Induced by Cantharidinate

A multidisciplinary approach to study the functional properties of neuron-like cell models constituting a living bio-hybrid system: SH-SY5Y cells adhering to PANI substrate

Nonlocal Effects in the Confocal μ-Raman Characterization of Inhomogeneous Polymer Coatings

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