Confocal Raman spectroscopy as a tool to assess advanced glycation end products on solar-exposed human skin

Pereira, Antônio Francisco Machado; Rodrigues, Bruno V. M.; Neto, Lázaro Pinto Medeiros; Lopes, Luana de Oliveira; Costa, Ana Adélya Alves; Santos, Alexandre Silva; Viana, Bartolomeu C.; Tosato, Maira Gaspar; Silva, G.C. da; Gusmão, G.O.M.; Fávero, P. P.; Martin, Aírton Abrahão

doi:10.1016/j.vibspec.2021.103234

Cited by 7 publications

(6 citation statements)

References 32 publications

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“…Confocal Raman spectroscopy (CRS) was used by Pereira et al ( 93 ) to determine the presence and differences in AGE content by identifying the reference spectra of the main AGEs present in human skin. This method can measure the content of AGEs in full-thickness skin tissue and explore the molecular mechanism of skin glycation through temporal and spatial spectral changes.…”

Section: Skin Advanced Glycation End Products Measurement Methodsmentioning

confidence: 99%

Advanced Glycation End Products in the Skin: Molecular Mechanisms, Methods of Measurement, and Inhibitory Pathways

Chen

Zhang

et al. 2022

Front. Med.

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Advanced glycation end products (AGEs) are a series of stable compounds produced under non-enzymatic conditions by the amino groups of biomacromolecules and the free carbonyl groups of glucose or other reducing sugars commonly produced by thermally processed foods. AGEs can cause various diseases, such as diabetes, atherosclerosis, neurodegeneration, and chronic kidney disease, by triggering the receptors of AGE (RAGEs) in the human body. There is evidence that AGEs can also affect the different structures and physiological functions of the skin. However, the mechanism is complicated and cumbersome and causes various harms to the skin. This article aims to identify and summarise the formation and characteristics of AGEs, focussing on the molecular mechanisms by which AGEs affect the composition and structure of normal skin substances at different skin layers and induce skin issues. We also discuss prevention and inhibition pathways, provide a systematic and comprehensive method for measuring the content of AGEs in human skin, and summarise and analyse their advantages and disadvantages. This work can help researchers acquire a deeper understanding of the relationship between AGEs and the skin and provides a basis for the development of effective ingredients that inhibit glycation.

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Section: Skin Advanced Glycation End Products Measurement Methodsmentioning

confidence: 99%

Advanced Glycation End Products in the Skin: Molecular Mechanisms, Methods of Measurement, and Inhibitory Pathways

Chen

Zhang

et al. 2022

Front. Med.

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“…In a recent study, advanced glycation end products as sign of photo-damage and skin ageing were successfully analysed in human biopsies by CRS. 102…”

Section: F I G U R E 5 Model Raman Spectra Of Fingerprint Region (Lef...mentioning

confidence: 99%

“…The observed spectral shift of the amide I peak of collagen in the dermis indicates structural changes and functional loss. In a recent study, advanced glycation end products as sign of photo‐damage and skin ageing were successfully analysed in human biopsies by CRS 102 …”

Section: Utilization Of Raman Spectroscopy In Skin Researchmentioning

confidence: 99%

Novel aspects of Raman spectroscopy in skin research

Lunter

Klang

Kocsis

et al. 2022

Experimental Dermatology

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The analytical technology of Raman spectroscopy has an almost 100‐year history. During this period, many modifications and developments happened in the method like discovery of laser, improvements in optical elements and sensitivity of spectrometer and also more advanced light detection systems. Many types of the innovative techniques appeared (e.g. Transmittance Raman spectroscopy, Coherent Raman Scattering microscopy, Surface‐Enhanced Raman scattering and Confocal Raman spectroscopy/microscopy). This review article gives a short description about these different Raman techniques and their possible applications. Then, a short statistical part is coming about the appearance of Raman spectroscopy in the scientific literature from the beginnings to these days. The third part of the paper shows the main application options of the technique (especially confocal Raman spectroscopy) in skin research, including skin composition analysis, drug penetration monitoring and analysis, diagnostic utilizations in dermatology and cosmeto‐scientific applications. At the end, the possible role of artificial intelligence in Raman data analysis and the regulatory aspect of these techniques in dermatology are briefly summarized. For the future of Raman Spectroscopy, increasing clinical relevance and in vivo applications can be predicted with spreading of non‐destructive methods and appearance with the most advanced instruments with rapid analysis time.

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“…In the analysis of biological tissues such as skin, the incident light excites the molecules of the sample, and the resulting signal is captured to form a spectrum, which represents a biochemical map of the human skin components. This technique has been used to analyze physiological parameters of the skin, such as cutaneous hydration 17 , detection of Advanced Glycation End Products (AGEs) at the dermis 18 and collagen glycation 19 , and even for cancer detection 20 . Furthermore, Confocal Raman Spectroscopy is a non-invasive method that can be used in vivo, providing high spatial resolution in microns (µm) and the ability to determine the permeation profile of formulations in different skin layers 21 .…”

Section: Introductionmentioning

confidence: 99%

In vivo permeation study of nanoencapsulated cannabidiol through the human skin by confocal Raman spectroscopy

Martin,

Fávero,

Carita

et al. 2024

Biomedical Vibrational Spectroscopy 2024: Advances in Research and Industry

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Chronic pain encompasses a range of painful conditions such as migraines, fibromyalgia, or prolonged periods of pain without a specific etiological cause. In this context, a less complex, easily applicable therapy that reduces side effects and does not require a high investment from the patient becomes highly important. Currently, chronic pain treatment with topically applied endocannabinoids has been a subject of research. However, permeation studies are necessary to ensure efficacy, safety, and delivery of the active ingredient to the skin layers. Confocal Raman Spectroscopy (CRS) has emerged as an analytical method for the analysis of materials, including biological tissues. In this context, the present study brings an in vivo approach using Confocal Raman Spectroscopy to analyze in vivo the permeation of two formulations containing Cannabidiol (CBD). One formulation consists of nanoencapsulated CBD in transferosomes (TRANS), while the other is a nanolipid carrier containing nanoencapsulated CBD in transferosomes (CLN TRANS). The aim of this study is to explore the topical treatment of chronic pain using these formulations and assess their permeation characteristics. Raman data were collected from stratum corneum, epidermis and dermis at T0, T4 and T8h after topical application. It was possible to identify that both products showed to permeate to the dermis, but with different kinetics.

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Confocal Raman spectroscopy as a tool to assess advanced glycation end products on solar-exposed human skin

Cited by 7 publications

References 32 publications

Advanced Glycation End Products in the Skin: Molecular Mechanisms, Methods of Measurement, and Inhibitory Pathways

Advanced Glycation End Products in the Skin: Molecular Mechanisms, Methods of Measurement, and Inhibitory Pathways

Novel aspects of Raman spectroscopy in skin research

In vivo permeation study of nanoencapsulated cannabidiol through the human skin by confocal Raman spectroscopy

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